Data for: Parallel processing of quickly and slowly mobilized reserve vesicles in hippocampal synapses
Data files
Mar 07, 2024 version files 10.18 GB
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README.md
Abstract
Vesicles within presynaptic terminals are thought to be segregated into a variety of readily releasable and reserve pools. The nature of the pools and trafficking between them is not well understood, but pools that are slow to mobilize when synapses are active are often assumed to feed pools that are mobilized more quickly, in a series. However, electrophysiological studies of synaptic transmission have suggested instead a parallel organization where vesicles within slowly and quickly mobilized reserve pools would separately feed independent reluctant- and fast-releasing subdivisions of the readily releasable pool. Here we use FM-dyes to confirm the existence of multiple reserve pools at hippocampal synapses and a parallel organization that prevents intermixing between the pools, even when stimulation is intense enough to drive exocytosis at the maximum rate. The experiments additionally demonstrate extensive heterogeneity among synapses in the relative sizes of the slowly and quickly mobilized reserve pools, which suggests equivalent heterogeneity in the numbers of reluctant and fast-releasing readily releasable vesicles that may be relevant for understanding information processing and storage.
README: Data for: Parallel processing of quickly and slowly mobilized reserve vesicles in hippocampal synapses
Data from individual preparations are contained in separate directories, each with a unique identifier; there are 191 in total. Each contains either two or three files (.tif, .sts, and usually .vls). The .tif file is a stack of aligned images. The .sts file contains two columns. The numbers in the first column are the median values of the mean value of several ROIs for each image. The second column is the mean value of an area designated as background. The .vls file contains 1 column for each ROI and the numbers are the mean values. The preparations used for generating each figure are listed below.
Please don't hesitate to contact me at john.wesseling@csic.es for help.
Figure 2
Figure2A (Example)
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Figure2B-E
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Figure 2–Figure Supplement 2
Three20HzTrains
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Two20HzTrains
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Figure 3
A, Example
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B-D n = 8 preparations
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Figure 4
Figure 4a (1 min)
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Figure 4b (3 min)
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Figure 4c (8 min)
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Figure 6
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Figure 7
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Figure 8
12sAT20Hz (a)
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60sAt20Hz (b)
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60sat1Hz (c)
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Figure 8–Figure Supplement 2
Load1Hz
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Load20Hz
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Figure 9
Load FM4-64 at 20Hz then 1-43 at 1Hz, Measure FM1-43 (n=8)
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Load FM4-64 at 20Hz then 1-43 at 1Hz, Measure FM4-64 (n=6)
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Load FM1-43 at 20Hz then 4-64 at 1Hz, Measure FM1-43 (n=7)
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Load FM1-43 at 20Hz then 4-64 at 1Hz, Measure FM4-64 (n=6)
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Figure 10
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Figure 10–Figure Supplement 1
A. 1Hz for 10 min then 20 Hz for 12 s n = 4
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B. 20Hz for 20s, then 13 min then 20Hz for 12s n = 3
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10 min delay then 20Hz for 20s, then 3 min then 20Hz for 12s n = 3
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Figure 11
Panel A
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Panel B
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Panel D
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Figure 11–Figure supplement 1
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Figure 11–Figure Supplement 2
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Figure 12
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Figure 12–Figure Supplement 1
Measure FM464
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Measure FM143
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