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Genetic insights into the globally invasive and taxonomically problematic tree genus Prosopis

Citation

Le Roux, Johannes (2020), Genetic insights into the globally invasive and taxonomically problematic tree genus Prosopis, Dryad, Dataset, https://doi.org/10.5061/dryad.zgmsbcc97

Abstract

Accurate taxonomic identification of alien species is crucial to detect new incursions, prevent or reduce the arrival of new invaders and implement management options such as biological control. Globally, the taxonomy of non-native Prosopis species is problematic due to misidentification and extensive hybridization. We performed a genetic analysis on several Prosopis species, and their putative hybrids, including both native and non-native populations, with a special focus on Prosopis invasions in Eastern Africa (Ethiopia, Kenya and Tanzania). We aimed to clarify the taxonomic placement of non-native populations and to infer the introduction histories of Prosopis in Eastern Africa. DNA sequencing data from nuclear and chloroplast markers showed high homology (almost 100%) between most species analysed. Analyses based on seven nuclear microsatellites confirmed weak population genetic structure among Prosopis species. Hybrids and polyploid individuals were recorded in both native and non-native populations. Invasive genotypes of P. juliflora in Kenya and Ethiopia could have a similar native Mexican origin, while Tanzanian genotypes likely are from a different source. Native Peruvian Prosopis pallida genotypes showed high similarity with non-invasive genotypes from Kenya. Levels of introduced genetic diversity, relative to native populations, suggest that multiple introductions of P. juliflora and P. pallida occurred to Eastern Africa. Polyploidy may explain the successful invasion of P. juliflora in Eastern Africa. The polyploid Prosopis juliflora was highly differentiated from the rest of the (diploid) species within the genus. The lack of genetic differentiation between most diploid species in their native ranges supports the notion that hybridization between allopatric species may occur frequently when they are co-introduced into non-native areas. For regulatory purposes, we propose to treat diploid Prosopis taxa from the Americas as a single taxonomic unit in non-native ranges.

Methods

Eleven previously-published nuclear microsatellite loci were amplified in a variety of Prosopis species. Amplified fragments were submitted for gel capillary electrophoresis at Stellenbosch University’s Central Analytical Facility. Automated allele scoring was done using the GeneMarker version 2.6.4 software (SoftGenetics LLC, Pennsylvania, United States) and all alleles were manually checked. The data is provided in GenAlex format (Peakall and Smouse 2012).

Reference: Peakall, R. and Smouse P.E. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28, 2537-2539.

Usage Notes

Missing data (alleles) are indicated as 0's. A separate tab in the spreadsheet provide metadat on location and species identities.

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Award: 400440_152085