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Amplicon_sorter: a tool for reference-free amplicon sorting based on sequence similarity and for building consensus sequences

Data files

This dataset is embargoed . Please contact Andy Vierstraete at eb.tnegu@eteartsreiV.ydnA with any questions.

Lists of files and downloads will become available to the public when released.

Abstract

Oxford Nanopore Technologies (ONT) is a third-generation sequencing technology that is gaining popularity in ecological research for its portable and low-cost sequencing possibilities. Although the technology excels at long-read sequencing, it can also be applied to sequence amplicons. The downside of ONT is the low quality of the raw reads. Hence, generating a high-quality consensus sequence is still a challenge. We present Amplicon_sorter, a tool for reference-free sorting of ONT sequenced amplicons based on their similarity in sequence and length and for building solid consensus sequences.