Data for: Drought and temperature stresses impact pollen production and autonomous selfing in a California wildflower, Collinsia heterophylla
Data files
Jul 07, 2023 version files 82.18 KB
-
Floral_traits_data_Arathi_and_Smith.csv
-
README.md.txt
-
VWC_data_Arathi_and_Smith.csv
Jan 08, 2024 version files 82.12 KB
-
Floral_traits_data_Arathi_and_Smith.csv
-
README.md
-
VWC_data_Arathi_and_Smith.csv
Abstract
Ongoing climatic changes have altered growing conditions for plants by limiting water availability and inducing unprecedented temperature increases, eliciting plant functional responses that compromise floral trait expression, reduce pollen production but promote early self-pollination. In a controlled greenhouse study, plants of Collinsia heterophylla, an annual mixed-mated hermaphrodite, were grown under temperature and water stresses. Floral trait responses and related effects on plant reproductive success were recorded. Plants grown under temperature and water stresses were shorter and had fewer leaves at flowering than control plants. Temperature-stressed plants flowered earlier and had smaller flowers that produced fewer per capita pollen grains than control and water-stressed plants. While lifetime flower production in plants experiencing temperature stress alone or water stress alone was similar to control plants, those receiving combined temperature and water stresses had significantly lower lifetime flower production. Abiotic stress did not affect investment in female traits such as ovule number per flower but impacted male traits seen as fewer pollen grains per flower. In plants experiencing abiotic stress, an increase in autonomous self-pollination was facilitated through a compromise in herkogamy, a sexual interference mechanism to promote cross-pollination. Spatial separation between stigma and anther, a measure of herkogamy, was zero or negative in stressed plants. Thus, abiotic stress responses in plants did not compromise plant reproductive success as autonomous selfing was augmented. In nature, such responses will likely reduce available nutrition for pollinators through decreased flower and pollen production.
README: Arathi and Smith (2023), Drought and temperature stresses impact pollen production and autonomous selfing in a California wildflower, Collinsia heterophylla, Dryad, Dataset, Dryad, Dataset, https://doi.org/10.5061/dryad.zgmsbcchr
The dataset contains floral trait measures and plant vegetative measures for the different abiotic stress treatments. The studies were conducted in a greenhouse growth chamber. There are no field sites associated with the data.
Methods of data collection: see manuscript for details
Description of the data and file structure
The data are in a spreadsheet format saved as a CSV/text file. The column headers that describe the traits are self-explanatory. For specific terms only relevant to this study or species, the definitions are provided in the published manuscript.
NA in the data file represents no data and should not be interpreted as 0.00.
File count: 2
File format: .csv
Details for: VWC data_Arathi and Smith.csv
Description: a comma-delimited file containing the soil water content (VWC) for all the treatments. The file can be reformatted for use with any other software.
Format: .csv
Size: 1 KB
Dimensions: 47 rows x 4 columns
Variables:
- trtnum: one-digit identification code for a treatment
- trtname: treatment: ND = normal temperature, dry; NA: Normal temperature, adequate water; HA: High temperature, adequate water; HD: High temperature, dry
- VWC: Soil water content in %
- Treatment names
Details for: Floral traits data_Arathi and Smith.csv
Description: a comma-delimited file containing the vegetative and reproductive trait measures for all the treatments. The file can be reformatted for use with any other software.
Format: .csv
Size: 80 KB
Dimensions: 535 rows x 33 columns
Variables:
- ID: two-digit alpha-numeric code for each replicate plant
- gc: growth chamber 1 or 2
- trtnum: one-digit identification code for a treatment
- Name: treatments in the study Control, heat, drought, Hot and dry
- Main treatment: temperature effect: normal or high
- sub treatment: watered or dry
- DOF: Date of flowering
- plht: plant height at flowering in cM
- leafnum: leaf number at flowering
- antherlen: length of anthers in each flower in mM
- stylelen: length of style in each flower in mM
- herkogamy: difference of style length and anther length in mM
- avgherk: average herkogamy in mM
- flowerlen: length of corolla from the base to the tip of the lower lip in mM
- avgflowerlen: average of flower length in mM
- flowerwid: width of flower in mM
- avgflowerwid: average of flower width in mM
- pollennum: total number of pollen grains per flower
- pollenvia: proportion of viable pollen per flower
- arcsinpollenviability: arc sin transformed value for proportion of viable pollen per flower
- pollentubelen: length of pollen tubes in the culture in µM
- seednum: seed numebr per fruit
- VWC: volumetric water content of soil in % in each pot
- flowernum: number of flowers per plant
- fruitnum: number of fruits per plant
- fruitabort: proportion of aborted fruits per plant
- asinfruitab: arc sin transformed value for proportion of fruits aborted per plant
- seedwtten: weight of ten seeds in mG
- Seedabprop: proportion of seeds aborted
- asinseedab: arc sin transformed value for proportion of seeds aborted
- seedtoovule: ratio of seed number to ovule number
- arcsinseedovule: arc sin transformed seed to ovule ratio
Methods
Dataset was collected by recording plant and floral traits by hand and collected data were entered into EXCEL worksheets for processing. IBM SPSS Statistics 21.0 (SPSS Inc. Chicago, IL) was used for statistical analyses.
Usage notes
The included data file can be opened with any spreadsheet software including MS Excel or Google Sheets.