58 leafhopper and 5 treehoppers species were selected to participate in the phylogenetic tree construction after the removal of unverified, lacks the accurate scientific name, and repeated species sequences. Phylogenetic analysis was performed using alignments of the 13 PCGs of leafhopper with the other complete or near complete genomes of the treehopper  species.  The two species of Cosmoscarta bispecularis (KP064511)  and  Tettigades auropilosa (KM000129) (Yan & Zu, 2019) were used as the outgroup.

The two complete mitogenomes of C. biungulata and C. robusta were sequenced by Berry Genomics on the HiSeq 2,500 platform (Illumina) with 150-bp paire end reads. The average insert length was 350 bp and 6 GB of clean data were obtained. Each mitogenome was assembled usingGeneious Prime 2019.2.1 software and based on a mitochondrial reference sequence of Olidiana ritcheriina (MK738125) (Wang et al., 2019b). The assembled mitochondrial gene sequences were compared with the homologous sequences of O. ritcheriina (MK738125) and Taharana fasciana (KY886913) , which were retrieved from GenBank and identified via BLAST searches in NCBI to confirm sequence accuracy. We used the MITOS web server  and BLAST searches in NCBI  to annotate the assembled sequences using invertebrate genetic codes.

(13PCG12, 1st and 2nd codons of 13 PCGs [6,676 bp]; the amino acid [AA] sequences of 13 PCGs [3,338 bp])