Herpes simplex viruses (HSVs) are highly pervasive and show a strong synergistic interaction with human immunodeficiency virus (HIV). 44 convenience samples were screened for HSV and HIV-1 using the highly sensitive enzyme-linked immunosorbent assay (ELISA). Of 44 samples, 81.8% were positive for HIV-1, while 79.5% were positive for HSV when screened with ELISA kits. The results of PCR with type specific primers showed that 11.4% samples were specific for HSV-1 while 85.7% were specific for HSV-2. Pearson’s chi-squared test established that there is a significant relationship between HSV-2 and HIV-1 transmission specific for HSV-1 while 85.7% were specific for HSV-2. Pearson’s chi-squared test established that there is a significant relationship between HSV-2 and HIV-1 transmission.