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Application of Hantzsch reaction for sensitive determination of eflornithine in cream, plasma and urine samples

Citation

Abdel-Lateef, mohamed; Almahri, Albandary (2021), Application of Hantzsch reaction for sensitive determination of eflornithine in cream, plasma and urine samples, Dryad, Dataset, https://doi.org/10.5061/dryad.zs7h44j7x

Abstract

Eflornithine is an anti-Trypanosoma Brucei agent for the medication of Sleeping Sickness and widely distributed for the treatment of hirsutism (unwanted facial hair in women). The presented work demonstrates a comprehensive analytical approach for the spectrofluorometric determination of eflornithine in the commercial cream samples and varied biological media. The proposed methodology is based on the formation of a highly yellow-green fluorescence dihydropyridine derivative after the interaction between eflornithine and acetylacetone/formaldehyde reagent in a slightly acidic medium. Furthermore, the optimal variables such as reagent volumes, pH of the medium, heating time, buffer volume, heating temperature and diluting solvent were carefully selected to achieve the maximum fluorescence activity. The fluorescence activity for the formed derivative was measured at λemission  =  477 nm after  λexcitation  =  418 nm. Concerning linearity, accuracy, sensitivity, preciseness and robustness, the presented work was validated and verified according to ICH guiding principles. Moreover, the proposed work offered a selective determination for eflornithine in various brands of pharmaceutical cream without any interference from excipients.  Eventually, the current approach was assured to be successful in the estimation of eflornithine in urine and plasma specimens with acceptable recovery results.