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Dryad

De novo genome assembly of human cell line CHM13 nanopore ultra-long reads using Shasta

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May 26, 2022 version files 5.91 GB

Abstract

Advances in Oxford Nanopore Technologies (ONT) sequencing, basecalling, and updates to Shasta are outpacing the publishing cycle. We aim to update users on the state of the art using the latest and greatest ONT data assembled with Shasta. This release encompassed our latest assembly of CHM13, the AssemblySummary.html and Assembly.gfa along with our evaluation presented in tables and figures. We assembled ultra-long nanopore reads of CHM13 using Shasta 0.9.0 with the iterative assembly mode to produce a haploid de novo genome assembly.