Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos

Albright, Ashley, University of California, Berkeley, https://orcid.org/0000-0002-7163-9640

ashleyalbright93@gmail.com

Published Nov 15, 2023 on Dryad. https://doi.org/10.6078/D13D9R

Cite this dataset

Albright, Ashley (2023). Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos [Dataset]. Dryad. https://doi.org/10.6078/D13D9R

Abstract

Using 10x Genomics Single-Cell Gene Expression (v3 chemistry) and the Illumina NovaSeq SP we sequenced RNA in nuclei isolated from control and maternal null CTCF pre-cellularization Drosophila melanogaster embryos, or nuclear cycle 14 and 9-13 OreR embryos.

Methods

Nuclei were isolated from control and dCTCF maternal null early to mid-nuclear cycle 14 Drosophila melanogaster embryos. Full protocol in associated publication.

Note (11/01/2023 upload): NC14 and NC9-13 data were not used in the linked manuscript; however, these are uploaded for use by the community.

Usage notes

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