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Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos

Citation

Albright, Ashley (2022), Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos, Dryad, Dataset, https://doi.org/10.6078/D13D9R

Abstract

Using 10x Genomics Single-Cell Gene Expression (v3 chemistry) and the Illumina NovaSeq SP we sequenced RNA in nuclei isolated from control and maternal null CTCF pre-cellularization Drosophila melanogaster embryos. 

Methods

Nuclei were isolated from control and dCTCF maternal null early to mid nuclear cycle 14 Drosophila melanogaster embryos.

Usage Notes

See README.txt for additional information. 

Funding