Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos
Data files
Aug 20, 2021 version files 50.28 GB
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AA4_6_S1_I1_001.fastq.gz
461.09 MB
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AA4_6_S1_R1_001.fastq.gz
1.54 GB
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AA4_6_S1_R2_001.fastq.gz
3.70 GB
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AA4_6_S2_I1_001.fastq.gz
413.98 MB
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AA4_6_S2_R1_001.fastq.gz
1.53 GB
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AA4_6_S2_R2_001.fastq.gz
3.65 GB
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AA4_6_S3_I1_001.fastq.gz
453.18 MB
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AA4_6_S3_R1_001.fastq.gz
1.45 GB
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AA4_6_S3_R2_001.fastq.gz
3.47 GB
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AA4_6_S4_I1_001.fastq.gz
496.06 MB
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AA4_6_S4_R1_001.fastq.gz
1.65 GB
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AA4_6_S4_R2_001.fastq.gz
3.94 GB
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AA4_9_S10_I1_001.fastq.gz
547.71 MB
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AA4_9_S10_R1_001.fastq.gz
1.78 GB
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AA4_9_S10_R2_001.fastq.gz
4.26 GB
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AA4_9_S11_I1_001.fastq.gz
585.80 MB
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AA4_9_S11_R1_001.fastq.gz
2.16 GB
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AA4_9_S11_R2_001.fastq.gz
5.17 GB
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AA4_9_S12_I1_001.fastq.gz
571.09 MB
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AA4_9_S12_R1_001.fastq.gz
1.68 GB
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AA4_9_S12_R2_001.fastq.gz
3.98 GB
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AA4_9_S9_I1_001.fastq.gz
512.93 MB
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AA4_9_S9_R1_001.fastq.gz
1.86 GB
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AA4_9_S9_R2_001.fastq.gz
4.41 GB
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README.txt
1.38 KB
Apr 27, 2022 version files 52.29 GB
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AA4_6_S1_I1_001.fastq.gz
461.09 MB
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AA4_6_S1_R1_001.fastq.gz
1.54 GB
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AA4_6_S1_R2_001.fastq.gz
3.70 GB
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AA4_6_S2_I1_001.fastq.gz
413.98 MB
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AA4_6_S2_R1_001.fastq.gz
1.53 GB
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AA4_6_S2_R2_001.fastq.gz
3.65 GB
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AA4_6_S3_I1_001.fastq.gz
453.18 MB
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AA4_6_S3_R1_001.fastq.gz
1.45 GB
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AA4_6_S3_R2_001.fastq.gz
3.47 GB
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AA4_6_S4_I1_001.fastq.gz
496.06 MB
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AA4_6_S4_R1_001.fastq.gz
1.65 GB
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AA4_6_S4_R2_001.fastq.gz
3.94 GB
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AA4_9_S10_I1_001.fastq.gz
547.71 MB
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AA4_9_S10_R1_001.fastq.gz
1.78 GB
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AA4_9_S10_R2_001.fastq.gz
4.26 GB
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AA4_9_S11_I1_001.fastq.gz
585.80 MB
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AA4_9_S11_R1_001.fastq.gz
2.16 GB
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AA4_9_S11_R2_001.fastq.gz
5.17 GB
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AA4_9_S12_I1_001.fastq.gz
571.09 MB
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AA4_9_S12_R1_001.fastq.gz
1.68 GB
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AA4_9_S12_R2_001.fastq.gz
3.98 GB
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AA4_9_S9_I1_001.fastq.gz
512.93 MB
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AA4_9_S9_R1_001.fastq.gz
1.86 GB
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AA4_9_S9_R2_001.fastq.gz
4.41 GB
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adata_b4batchcorrect.h5ad
189.37 MB
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adata_corrected.h5ad
1.23 GB
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control_adata.h5ad
268.97 MB
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ctcf_adata.h5ad
323.86 MB
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README.txt
1.38 KB
Nov 15, 2023 version files 54.50 GB
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AA1006_S1_L001_R1_001.fastq.gz
606.91 MB
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AA1006_S1_L001_R2_001.fastq.gz
1.41 GB
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AA1006_S2_L001_R1_001.fastq.gz
770.80 MB
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AA1006_S2_L001_R2_001.fastq.gz
1.80 GB
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AA1006_S3_L001_R1_001.fastq.gz
789.22 MB
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AA1006_S3_L001_R2_001.fastq.gz
1.84 GB
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AA1006_S4_L001_R1_001.fastq.gz
634.11 MB
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AA1006_S4_L001_R2_001.fastq.gz
1.47 GB
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AA1016_S5_L001_R1_001.fastq.gz
1.49 GB
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AA1016_S5_L001_R2_001.fastq.gz
3.54 GB
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AA1016_S6_L001_R1_001.fastq.gz
1.69 GB
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AA1016_S6_L001_R2_001.fastq.gz
4.03 GB
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AA1016_S7_L001_R1_001.fastq.gz
1.35 GB
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AA1016_S7_L001_R2_001.fastq.gz
3.26 GB
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AA1016_S8_L001_R1_001.fastq.gz
1.61 GB
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AA1016_S8_L001_R2_001.fastq.gz
3.84 GB
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AA7-1_S1_L001_R1_001.fastq.gz
1.12 GB
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AA7-1_S1_L001_R2_001.fastq.gz
2.48 GB
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AA7-1_S2_L001_R1_001.fastq.gz
813.88 MB
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AA7-1_S2_L001_R2_001.fastq.gz
1.81 GB
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AA7-1_S3_L001_R1_001.fastq.gz
968.77 MB
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AA7-1_S3_L001_R2_001.fastq.gz
2.15 GB
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AA7-1_S4_L001_R1_001.fastq.gz
678.39 MB
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AA7-1_S4_L001_R2_001.fastq.gz
1.50 GB
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AA7-2_S5_L001_R1_001.fastq.gz
1.13 GB
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AA7-2_S5_L001_R2_001.fastq.gz
2.36 GB
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AA7-2_S6_L001_R1_001.fastq.gz
929.46 MB
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AA7-2_S6_L001_R2_001.fastq.gz
1.94 GB
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AA7-2_S7_L001_R1_001.fastq.gz
1.04 GB
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AA7-2_S7_L001_R2_001.fastq.gz
2.14 GB
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AA7-2_S8_L001_R1_001.fastq.gz
1.08 GB
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AA7-2_S8_L001_R2_001.fastq.gz
2.20 GB
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README.txt
2.20 KB
Abstract
Using 10x Genomics Single-Cell Gene Expression (v3 chemistry) and the Illumina NovaSeq SP we sequenced RNA in nuclei isolated from control and maternal null CTCF pre-cellularization Drosophila melanogaster embryos, or nuclear cycle 14 and 9-13 OreR embryos.
Methods
Nuclei were isolated from control and dCTCF maternal null early to mid-nuclear cycle 14 Drosophila melanogaster embryos. Full protocol in associated publication.
Note (11/01/2023 upload): NC14 and NC9-13 data were not used in the linked manuscript; however, these are uploaded for use by the community.
Usage notes
See README.txt for additional information.