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Compartmentalization of cerebrospinal fluid inflammation across the spectrum of HIV infection

Cite this dataset

Price, Richard et al. (2021). Compartmentalization of cerebrospinal fluid inflammation across the spectrum of HIV infection [Dataset]. Dryad.


Objective: To characterize the evolution of central nervous system (CNS) inflammation in HIV-1 infection applying a panel of cerebrospinal fluid (CSF) inflammatory biomarkers to grouped subjects representing a broad spectrum of systemic HIV-1 immune suppression, CNS injury and viral control.

Methods: This is a cross-sectional analysis of archived CSF and blood samples, assessing concentrations of 10 functionally diverse soluble inflammatory biomarkers by immunoassays in 143 HIV-1-infected subjects divided into 8 groups: untreated primary HIV-1 infection (PHI); four untreated groups defined by their blood CD4+ T lymphocyte counts; untreated patients presenting with subacute HIV-associated dementia (HAD); antiretroviral-treated subjects with >1 years of plasma viral suppression; and untreated elite controllers. Twenty HIV-1-uninfected controls were included for comparison. Background biomarkers included blood CD4+ and CD8+ T lymphocytes, CSF and blood HIV-1 RNA, CSF white blood cell (WBC) count, CSF/blood albumin ratio, CSF neurofilament light chain (NfL), and CSF t-tau.

Findings: HIV-1 infection was associated with a broad compartmentalized CSF inflammatory response that developed early in its course and changed with systemic disease progression, development of neurological injury, and viral suppression. CSF inflammation in untreated individuals without overt HAD exhibited at least two overall patterns of inflammation as blood CD4+ T lymphocytes decreased: one that peaked at 200-350 blood CD4+ T cells/µL and associated with lymphocytic CSF inflammation and HIV-1 RNA concentrations; and a second that steadily increased through the full range of CD4+ T cell decline and associated with macrophage responses and increasing CNS injury. Subacute HAD was distinguished by a third inflammatory profile with increased blood-brain barrier permeability and robust combined lymphocytic and macrophage CSF inflammation. Suppression of CSF and blood HIV-1 infections by antiretroviral treatment and elite viral control were associated with reduced CSF inflammation, though not fully to levels found in HIV-1 seronegative controls.


CSF was collected by lumbar puncture and plsama/serum by phlebotomy. CSF was placed immediately on wet ice and subsequently subjected to low-speed centrifugation to remove cells, aliquoted and stored within 2 hours of collection at <-70°C until the time of HIV-1 RNA and biomarker assays. Blood was collected either in EDTA or as serum, aliquoted and stored in parallel with CSF for later batch assays. Details are outlined in the manuscript derived from this data.

Usage notes

This dataset details the results of inflammatory biomarker measurements and background biomarkers in CSF and blood in a spectrum of HIV-infected individuals along with uninfected controls. The samples were selected from archived samples collected in San Francisco and Gothenburg as detailed in the parent manuscript.


National Institute of Neurological Disorders and Stroke, Award: R01 NS094067 and R01 NS094067-05S1

National Institute of Mental Health, Award: R21 MH096619

National Institute of Mental Health, Award: P01 MH094177

National Institute of Mental Health, Award: R01 MH081772