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AlleleAnalyzer supporting data

Cite this dataset

Keough, Kathleen et al. (2019). AlleleAnalyzer supporting data [Dataset]. Dryad.


The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is open-source software that incorporates single nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing one or multiple haplotypes of a sequenced genome, currently supporting eleven Cas proteins.  It also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations. AlleleAnalyzer is available at


Please see readme and manuscript. 

Usage notes

Please see readme and manuscript.