SARS-CoV-2-specific T cells exhibit phenotypic features reflecting helper function, lack of terminal differentiation, and high proliferation potential
Data files
Jun 25, 2020 version files 430.21 MB
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PID4100_singlets_CD4928_CD3_.fcs
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PID4100_singlets_CMVP_CD3_.fcs
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PID4100_singlets_CovidP_CD3_.fcs
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PID4101_singlets_CD4928_CD3_.fcs
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PID4101_singlets_CMVP_CD3_.fcs
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PID4101_singlets_CovidP_CD3_.fcs
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PID4102_singlets_CD4928_CD3_.fcs
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PID4102_singlets_CMVP_CD3_.fcs
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PID4102_singlets_CovidP_CD3_.fcs
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PID4103_singlets_CD4928_CD3_.fcs
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PID4103_singlets_CMVP_CD3_.fcs
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PID4103_singlets_CovidP_CD3_.fcs
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PID4105_singlets_CD4928_CD3_.fcs
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PID4105_singlets_CMVP_CD3_.fcs
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PID4105_singlets_CovidP_CD3_.fcs
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PID4106_singlets_CD4928_CD3_.fcs
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PID4106_singlets_CMVP_CD3_.fcs
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PID4106_singlets_CovidP_CD3_.fcs
Nov 09, 2020 version files 1 GB
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PID4100_singlets_CD4928_CD3_.fcs
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PID4100_singlets_CMVP_CD3_.fcs
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PID4100_singlets_CovidP_CD3_.fcs
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PID4101_singlets_CD4928_CD3_.fcs
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PID4101_singlets_CMVP_CD3_.fcs
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PID4101_singlets_CovidP_CD3_.fcs
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PID4102_singlets_CD4928_CD3_.fcs
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PID4102_singlets_CMVP_CD3_.fcs
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PID4102_singlets_CovidP_CD3_.fcs
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PID4103_singlets_CD4928_CD3_.fcs
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PID4103_singlets_CMVP_CD3_.fcs
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PID4103_singlets_CovidP_CD3_.fcs
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PID4104_singlets_CD4928_CD3_.fcs
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PID4104_singlets_CMVP_CD3_.fcs
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PID4104_singlets_CovidP_CD3_.fcs
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PID4105_singlets_CD4928_CD3_.fcs
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PID4105_singlets_CMVP_CD3_.fcs
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PID4105_singlets_CovidP_CD3_.fcs
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PID4106_singlets_CD4928_CD3_.fcs
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PID4106_singlets_CMVP_CD3_.fcs
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PID4106_singlets_CovidP_CD3_.fcs
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PID4107_singlets_CD4928_CD3_.fcs
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PID4107_singlets_CMVP_CD3_.fcs
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PID4107_singlets_CovidP_CD3_.fcs
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PID4108_singlets_CD4928_CD3_.fcs
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PID4108_singlets_CMVP_CD3_.fcs
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PID4108_singlets_CovidP_CD3_.fcs
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PID4109_singlets_CD4928_CD3_.fcs
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PID4109_singlets_CMVP_CD3_.fcs
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PID4109_singlets_CovidP_CD3_.fcs
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PID4110_singlets_CD4928_CD3_.fcs
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PID4110_singlets_CMVP_CD3_.fcs
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PID4110_singlets_CovidP_CD3_.fcs
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PID4114_singlets_CD4928_CD3_.fcs
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PID4114_singlets_CMVP_CD3_.fcs
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PID4114_singlets_CovidP_CD3_.fcs
Abstract
Convalescing COVID-19 patients mount robust T cell responses against SARS-CoV-2, suggesting an important role for T cells in viral clearance. To date, the phenotypes of SARS-CoV-2-specific T cells remain poorly defined. Using 38-parameter CyTOF, we phenotyped longitudinal specimens of SARS-CoV-2-specific CD4+ and CD8+ T cells from nine individuals who recovered from mild COVID-19. SARS-CoV-2-specific CD4+ T cells were exclusively Th1 cells, and predominantly Tcm with phenotypic features of robust helper function. SARS-CoV-2-specific CD8+ T cells were predominantly Temra cells in a state of less terminal differentiation than most Temra cells. Subsets of SARS-CoV-2-specific T cells express CD127, can homeostatically proliferate, and can persist for over two months. Our results suggest that long-lived and robust T cell immunity is generated following natural SARS-CoV-2 infection, and support an important role for SARS-CoV-2-specific T cells in host control of COVID-19.