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Dryad

Database of human alternative 5' and 3' splice sites

Data files

Aug 24, 2022 version files 2.43 MB

Abstract

To determine the impact of the intron-exon architecture and splice site strength on splice site selection, we created a database of alternative splice sites (ALTssDB) using the Human Exon Splicing Event Database HEXEvent, the Intron DB, and GeneBase. MaxEntScan, a computational tool was used to assign splice site scores. To minimize variability, we focused on competing alternative 5′ or 3′ splice site pairs of internal exons with only one alternative splice pattern. Thus, two alternative 5′ splice sites compete for a common 3′ss, or two alternative 3′ splice sites compete for a common 5′ss. As a result, ALTssDB reports the location of the major splice site and its competing alternative 3′ or 5′ splice site, corresponding exon sizes, usage levels, splice site scores and flanking intron lengths.