Title: Distinct type II opsins in the eye decode light properties for background adaptation and behavioural background preference Authors Gabriel E. Bertolesi Nilakshi Debnath Karen Atkinson-Leadbeater Anna Niedzwiecka Sarah McFarlane Figure S 1 B/D Correlation Background preference and background adaptation of tadpoles tretaed with aMSH and melatonin Figuire 1S 1 B "White background preference for tadpoles adapted to White Background or Black Background, measured as a percentage of time spent on a white surface." Exp: 2019-8 Correlation Back Pref with Pigm aMSH and Melatonin time 840 WB control 840 WB +aMSH 840 BB control 840 BB + Melat. Embr. # WB BB %WB %BB WB BB %WB %BB WB BB %WB %BB WB BB %WB %BB 1 484 356 57.62 42.86 808 32 96.19 3.81 310 530 36.90 63.10 724 116 86.19 13.81 2 716 124 85.24 14.76 360 480 42.86 57.14 384 456 45.71 54.29 132 708 15.71 84.29 3 720 120 85.71 14.29 480 360 57.14 42.86 440 400 52.38 47.62 268 572 31.90 68.10 4 739 101 87.98 12.02 600 240 71.43 28.57 337 503 40.12 59.88 375 465 44.64 55.36 5 547 293 65.12 34.88 690 150 82.14 17.86 565 275 67.26 32.74 449 391 53.45 46.55 6 480 360 57.14 42.86 531 309 63.21 36.79 414 426 49.29 50.71 304 536 36.19 63.81 7 780 60 92.86 7.14 720 120 85.71 14.29 580 260 69.05 30.95 387 453 46.07 53.93 8 701 139 83.45 16.55 840 0 100.00 0.00 479 361 57.02 42.98 240 600 28.57 71.43 9 519 321 61.79 38.21 670 170 79.76 20.24 366 474 43.57 56.43 120 720 14.29 85.71 average 75.21 75.38 51.26 39.67 Figure S 1 D Assessment of Skin Pigmentation Exp: 2019-8 Correlation Back Pref with Pigm aMSH and Melatonin Pigmentation Embr. # WB WB + aMSH BB BB + Mel. 1 5398.02 6770.31 7358.91 2208.75 2 3308.52 7498.20 8059.96 2851.39 3 5129.75 7045.30 6803.57 1334.48 4 5917.82 7672.29 7807.49 1762.44 5 6018.50 7747.82 7960.67 1876.43 6 3493.08 7548.43 7551.28 2350.68 7 4244.00 7500.02 8016.60 1953.21 8 2824.41 7470.27 7746.29 2526.96 9 5364.17 7809.46 7414.48 3595.73 average 4633.141444 7451.343556 7635.471444 2273.341778