This readme.txt file was generated on 2021-09-30 by Mitch Cruzan GENERAL INFORMATION 1. Title of Dataset: Weak Coupling among Barrier Loci and Waves of Neutral and Adaptive Introgression Across an Expanding Hybrid Zone 2. Author Information A. Principal Investigator Contact Information Name: Mitch Cruzan Institution: Portland State University Address: Portland, Oregon Email: cruzan@pdx.edu 3. Dates of data collection: 2015 - 2018 4. Geographic location of data collection: Jackson County, Oregon, USA 5. Information about funding sources that supported the collection of the data: NSF Macrosystems Biology award NSF-MSB-1340746 to MBC DATA & FILE INFORMATION 1. File List: all Species(Ranocc= Ranunculus occidentalis, Ranaus= R. austro-oreganus). Hybrid is for phenotypically intermediate individuals (based on ventral petal color) Name: Ranunc_enm_bioclime_data.csv Methods: PRISM 30-year normal data (https://prism.oregonstate.edu/) used for environmental niche modeling. Variables(description): ID(location ID), Species(Ranocc= Ranunculus occidentalis, Ranaus= R. austro-oreganus), Long(longitude), Lat(latitude), AET(elevation), Annual(precipitation), Aspect (for location), Prism1-21 (Prism biolimatic variables) Name: Ranunc_Flowering.csv Methods: Data are based on personal observations of flowering, or were downloaded from herbarium databases. Downloaded data were filtered for accurate location information. Records lacking accurate location descriptions were eliminated. Variables(description): UniqueNum(id number), KatieG id(alt id), Observation(N=specimen collected), Accession(herbarium id number), Species(Ranocc= Ranunculus occidentalis, Ranaus= R. austro-oreganus), Source(Cruzan pers observation, Consortium of Pacific Northwest Herbaria, Oregon Flora Project), Day, Month, Year, Date, Julian Day, Long, Lat, DEM(elevation in meters) Name: Petal Data.csv Methods: Image analysis using ImageJ. Color images were converted to monochrome. Variables(description): Population(name), Species(as above), Label(sample id), Mean_Gray(pixels), StdDev(mean_Gray), Min, Max, PercentLight(proportion non-white) Name: Leaf trichome data.csv Methods: Image analysis using ImageJ. Color images were converted to monochrome. Variables(description): label(sample label), Species(as above), Population(name), Lat, Long, Envelope(alt sample id), Basal vs Cauline(leaf position), Ave. Gray.Scale, Hair Coverage (segmentation)(proportion not white) Name: HZAR Output Summary.csv Methods: Output from HZAR analysis in R using for the 50 SNP loci fitting cline models Variables: ID(number), Locus(SNP location in pseudo reference), center(relative to contact based on the average centers of neutral clines), width(cline), cent/wid(ratio), C=occidentalis, A=aurtro-oreganus Name: Field seed set data.csv Methods: Clusters of achenes were collected from plants in the field. Individual achenes characterized as filled, unfertilized, or aborted. Variables: species(as above), pop(name), individual(witihin population), ID(sample name), fert_seeds(filled achenes), unfert_ovules(unfertilized), abort_ovules(aborted), total_ovules(sum of filled, abort, and unfert), premature(achenes not fully mature) Name: SNP 50 loci fitting cline models.csv Methods: Genotyping by Sequencing SNP data for individual plants. 50 SNP loci fitting cline models from HZAR. Variables: ID(label), Pop(number), Loc1-Loc50 Name: Greenhouse flowering data.csv Methods: Flowering of offspring from self, outcross, and interspecific crosses recorded. Variables: ID(label), MotherSite(origin of seed for maternal plant), Flower(yes or no), spTreatment(cross type: Species_Cross. Species as above. inter= between species, self=within plant geitonogamy, out=between populations within species) Name: Ranunc_Greenhouse_SeedCounts.csv Methods: Greenhouse crosses conducted with plants grown from field-collected seeds. Variables: ID(envelope label), spTreatment_Mom(out, self, interspecific), Species_Mom(seed parent species), MotherSite_Indvl-Pot-Plant(seed parent plant label), MotherSite(seed souce seed parent), DateEmasculated(date anthers removed), Treatment(outcross, self, interspecific), DateOfTreatment(cross date), spTreatment_Dad(pollen donor species), FatherSite_Indvl-Pot-Plant(plant label), FatherSite(seed source pollen donor), FatherSite_New(seed source pollen donor), Fert(filled+aborted), Filled(achene), Unfilled(aborted), SmallGreen(aborted), Aborted(unfilledandsmallgreen), SmallYellow(unfertilized), Total(total achenes), perc(% fillled seeds) Name: Germination data.csv Methods: Seeds from each achene cluster from greenhouse crosses were planted in soil in the greenhouse. Number of seedlings counted. Variables: ID(envelope label), MotherSite_Indvl-Pot-Plant(seed parent plant label), MotherSite(seed parent source), seedsPlanted(number of achenes planted), totGerminated(number of seedlings), germPerc(% germination), spTreatment(Species as above, treatment as above), spMother(seed parent species), cross(self, outcross, interpecific)