This readme.txt file was generated on 2021-10-14 by Matthias Pilecky, PhD GENERAL INFORMATION 1. Title of Dataset: Hydrogen isotopes (d2H) of polyunsaturated fatty acids track bioconversion by zooplankton 2. Author Information A. Principal Investigator Contact Information Name: Martin Kainz, PhD Institution: WasserCluster Lunz - GmbH Address: Dr. Carl-Kupelwieser-Promenade 5 Email: martin.kainz@donau-uni.ac.at B. Associate or Co-investigator Contact Information Name: Matthias Pilecky, PhD Institution: WasserCluster Lunz - GmbH Address: Dr. Carl-Kupelwieser-Promenade 5 Email: matthias.pilecky@donau-uni.ac.at 3. Date of data collection (single date, range, approximate date): 2020-06-01 - 2020-09-30 4. Geographic location of data collection: Water, dietary plankton (seston) and zooplankton were collected monthly from nine shallow (1-2 m depth) fishponds located in northern Austria around Waidhofen/Thaya (48° 49′ N, 15° 17′ E, 510 m) 5. Information about funding sources that supported the collection of the data: This work has been supported by the Austrian Science Fund (FWF; I-3855) and the German Research Foundation (DFG; MA 5005/8-1) within the framework of the DACH collaboration (project ‘AquaTerr’). DATA & FILE OVERVIEW 1. File List: d2H_FieldStudy.csv (Isotopic Data from the FieldStudy) d2H_LabStudy.csv (Isotopic Data from the LabStudy) 2. Relationship between files, if important: Field and Lab Study Data from: Pilecky M., Kämmer, S., Mathieu-Resuge, M., Taipale, S., Wassenaar, L.I., Martin-Creuzburg, D., Kainz, M. (2021): H-CSIA reveals LC-PUFA buffering capacity of zooplankton in eutrophic freshwater ecosystems. Functional Ecology. 3. Additional related data collected that was not included in the current data package: Fatty acid quantification data can be found as supplementary information in the publication above or upon request to the corresponding author METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: See: Pilecky M., Kämmer, S., Mathieu-Resuge, M., Taipale, S., Wassenaar, L.I., Martin-Creuzburg, D., Kainz, M. (2021): H-CSIA reveals LC-PUFA buffering capacity of zooplankton in eutrophic freshwater ecosystems. Functional Ecology. 2. Methods for processing the data: See: Pilecky M., Kämmer, S., Mathieu-Resuge, M., Taipale, S., Wassenaar, L.I., Martin-Creuzburg, D., Kainz, M. (2021): H-CSIA reveals LC-PUFA buffering capacity of zooplankton in eutrophic freshwater ecosystems. Functional Ecology. 3. Instrument- or software-specific information needed to interpret the data: Standard .csv reader 4. Standards and calibration information, if appropriate: USGS70, USGS71 and USGS72 (C20-Me with certified d2H and d13C values) 5. Environmental/experimental conditions: See: Pilecky M., Kämmer, S., Mathieu-Resuge, M., Taipale, S., Wassenaar, L.I., Martin-Creuzburg, D., Kainz, M. (2021): H-CSIA reveals LC-PUFA buffering capacity of zooplankton in eutrophic freshwater ecosystems. Functional Ecology. 6. Describe any quality-assurance procedures performed on the data: See: Pilecky M., Winter K., Wassenaar L.I., and Kainz M.J. (2020). Compound-specific deuterium analysis of lipids: applications for trophic ecology. Rapid Com Mass Spectro 35(16): e9135. 7. People involved with sample collection, processing, analysis and/or submission: Matthias Pilecky, Martin Kainz, Katharina Winter, Lena Fehlinger, Eric Wassenaar, Margaux Mathieu-Resuge, Samuel Karl-Kämmer DATA-SPECIFIC INFORMATION FOR: d2H_LabStudy.csv 1. Number of variables: 1 2. Number of cases/rows: 7 3. Variable List: ID... Sample ID Species... Fatty acid compound d2H... d2H value of Fatty acid compound Type... Diet (Algae) or Daphnia Fed... Treatment: Cryptomonas (high LC-PUFA) or Scenedesmus/Acutodesmus (no LC-PUFA) Deut... Labeling: 0 µg/mL (Diet) = Control, 25/50 µg/mL (Diet) = labelled Diet, 50 µg/mL (Daphnia) = enriched ambient water of Daphnids Generation... either Diet, or Daphnia Generation 1 or Daphnia Generation 2 DATA-SPECIFIC INFORMATION FOR: d2H_FieldStudy.csv 1. Number of variables: 1 2. Number of cases/rows: 7 3. Variable List: ID... Sample ID Species... Fatty acid compound d2H... d2H value of Fatty acid compound Pond... Pondname (Abbreviation: Jag... Jägerteich, NL... Furtteich, Durn... Dürnhofteich, Kieb... Kiebitzteich, Stadt... Stadtteich, Eng... Engelbrechtsteich, Ger... Gerhardtsteich, Dach... Dachetteich, Herr... Herrenteich) Season... Month of sample collection Type... Seston/Zooplankton ZooType... Specific typ of sample (e.g. bulk zooplankton, Daphnia, etc...)