These directories contain raw image files for the article: Gray, M.E., Johnson, Z.R., Modak, D., Tamilselvan, E., Tyska, M.J., Sotomayor, M. Heterophilic and homophilic cadherin interactions in intestinal intermicrovillar links are species dependent (awaiting acceptance in PLOS Biology). Comments and requests should be addressed to Dr. Marcos Sotomayor: sotomayor.8@osu.edu Directories below contain brightfield microscopy images (TIF files) for the homophilic bead aggregation assays corresponding to the supplementary data files in the supplementary information (S1 to S5 Data). Images can be opened in a variety of software. Below is a description of each compressed folder: Directory name Size Description S1_Data_Raw_Images_1.zip 6.59 GB Images for the homophilic hsPCDH24 bead aggregation assays for the constructs EC1, EC1-2, EC1-3, and EC1-4. S1_Data_Raw_Images_2.zip 5.39 GB Images for the homophilic hsPCDH24 bead aggregation assays for the constructs EC1-5, EC1-6, EC1-7, and EC1-MAD10. S2_Data_Raw_Images_1.zip 8.04 GB Images for the homophilic hsPCDH24 mutant bead aggregation assays for the constructs EC1-2 WT, Y67A, and Y71A. S2_Data_Raw_Images_2.zip 8.91 GB Images for the homophilic hsPCDH24 mutant bead aggregation assays for the constructs EC1-3 WT, Y67A, and Y71A. S3_Data_Raw_Images.zip 2.69 GB Images for the homophilic bead aggregation assays of hsPCDH24 EC1-MAD10 and mmPCDH24 EC1-MAD10 comparison. S4_Data_Raw_Images.zip 2.69 GB Images for the homophilic bead aggregation assays of hsCDHR5 EC1-4 and mmCDHR5 EC1-4 comparison. S5_Data_Raw_Images.zip 6.30 GB Images for the homophilic mmCDHR5 bead aggregation assays for the constructs EC1, EC1-2, EC1-3, EC1-4, EC1-4 R82G and EC1-4 E84G. The image files are organized into folders based on construct (EC1, EC1-2, EC1-3, etc), replicate (replicate 1, 2, etc), timepoint during the assay (T0, T60, R1, etc), and the presence of calcium (CA) or EDTA. Directories below contain fluorescent microscopy images (16-bit TIF files) for the heterophilic bead aggregation assays corresponding to the supplementary data files in the supplementary information (S6 and S7 Data). Images can be opened in ImageJ or Nikon Elements software. Below is a description of each compressed folder: Directory name Size Description S6_Data_Raw_Images_1.zip 9.08 GB Images for the heterophilic bead aggregation assays of truncations of hsCDHR5 (EC1, EC1-2 and EC1-3) mixed with hsPCDH24 EC1-4. S6_Data_Raw_Images_2.zip 6.45 GB Images for the heterophilic bead aggregation assays of truncations of hsPCDH24 (EC1, EC1-2 and EC1-3) mixed with hsCDHR5 EC1-4. S6_Data_Raw_Images_3.zip 5.74 GB Images for the heterophilic bead aggregation assays of hsPCDH24 (EC1-4 and EC1-MAD10) mixed with hsCDHR5 EC1-4. S7_Data_Raw_Images_1.zip 8.61 GB Images for the heterophilic bead aggregation assays of truncations of mmCDHR5 (EC1, EC1-2, EC1-3 and EC1-4) mixed with mmPCDH24 EC1-4. S7_Data_Raw_Images_2.zip 8.61 GB Images for the heterophilic bead aggregation assays of truncations of mmPCDH24 (EC1, EC1-2, EC1-3 and EC1-MAD10) mixed with mmCDHR5 EC1-4. S11_Data_Raw_Images.zip 4.75 GB Images for the heterophilic bead aggregation assays of minimum binding units of human and mouse PCDH24 and CDHR5 interaction. S12_Data_Raw_Images.zip 4.30 GB Images for the heterophilic bead aggregation assays of EDTA controls for human and mouse full-length cadherin ectodomains of PCDH24 (EC1-MAD10) and CDHR5 (EC1-4) The image files are organized into folders based on the interactions (EC1, EC1-2, EC1-3, etc), replicate (replicate 1, 2, etc), timepoint during the assay (T0, T60, R1, etc), and the presence of calcium (CA) or EDTA. Each folder has the same image captured with GFP and Texas red filter.