2020.06.12 Replicate 1 <> 1 2 3 4 5 6 7 8 A 0.3558 0.3097 0.2363 0.185 0.1383 0.0958 B 1.2241 0.1902 0.109 0.1035 0.1026 C 0.4035 0.3411 0.377 0.3172 0.4671 0.2135 0.2854 D E F G H "*spun down 1mL of cells, froze ON, added 200uL buffer, sonicated, proteinaseK, carbohydrate assay" CFU/mL ug ug/uL mg/CFU ug/CFU M90T 9000000000 0.4035 0.3077 23.75984991 2.375984991 2.63998E-10 2.63998E-07 ompA 8000000000 0.3411 0.2453 19.07692308 1.907692308 2.38462E-10 2.38462E-07 R1 5200000000 0.377 0.2812 21.77110694 2.177110694 4.18675E-10 4.18675E-07 R2 5400000000 0.3172 0.2214 17.28330206 1.728330206 3.20061E-10 3.20061E-07 R3 1600000000 0.4671 0.3713 28.53283302 2.853283302 1.7833E-09 1.7833E-06 R4 900000000 0.2135 0.1177 9.500938086 0.950093809 1.05566E-09 1.05566E-06 R5 540000000 0.2854 0.1896 14.89681051 1.489681051 2.75867E-09 2.75867E-06 ug/well glucose Measured' Blanked Glucose Standard Curve 20 0.3558 0.26 0.26 Glucose Standard Curve 16 0.3097 0.2139 Glucose Standard Curve 12 0.2363 0.1405 Glucose Standard Curve 8 0.185 0.0892 Glucose Standard Curve 4 0.1383 0.0425 Glucose Standard Curve 0 0.0958 0 2020.06.15 Replicate 2 <> 1 2 3 4 5 6 7 8 A 0.0908 0.1491 0.1842 0.2743 0.4263 0.5697 B 0.1327 0.1899 0.1739 0.1809 0.1695 0.1761 0.1813 C D E F G H "* spun down 1mL, extracted LPS in 200uL of buffer, measured 10 uL of final solution" CFU/mL LPS Measurement Blanked ug ug/uL mg/CFU ug/CFU M90T 2.00E+09 0.1327 0.0419 3.680115795 0.36801158 1.84E-10 1.84E-07 ompA 3.50E+09 0.1899 0.0991 6.09492793 0.609492793 1.74E-10 1.74E-07 R1 2.30E+09 0.1739 0.0831 5.419456004 0.5419456 2.36E-10 2.36E-07 R2 3.50E+09 0.1809 0.0901 5.714974971 0.571497497 1.63E-10 1.63E-07 R3 6.40E+08 0.1695 0.0787 5.233701224 0.523370122 8.18E-10 8.18E-07 R4 3.30E+08 0.1761 0.0853 5.512333394 0.551233339 1.67E-09 1.67E-06 R5 9.00E+07 0.1813 0.0905 5.731861769 0.573186177 6.37E-09 6.37E-06 ug/well glucose Measured' Blanked Glucose Standard Curve 20 0.5697 0.4789 Glucose Standard Curve 16 0.4263 0.3355 Glucose Standard Curve 12 0.2743 0.1835 Glucose Standard Curve 8 0.1842 0.0934 Glucose Standard Curve 4 0.1491 0.0583 Glucose Standard Curve 0 0.0908 0 2020.06.26 Replicate 3 <> 1 2 3 4 5 6 7 8 A 0.0925 0.1984 0.2872 0.3807 0.4294 0.5483 B C 0.3842 0.3562 0.3973 0.3159 0.2085 0.3141 0.2099 D E F G H "v sput down 1 mL of cells, froze ON, added 250 uL buffer, sonicated, proteinase K, carbohydrate assay" CFU/mL Measured Blanked ug mg/pellet mg/CFU ug/CFU M90T 3200000000 0.3842 0.2917 12.80639374 0.3202 1.00063E-10 1.00063E-07 ompA 3800000000 0.3562 0.2637 11.52764639 0.2882 7.58421E-11 7.58421E-08 R1 2400000000 0.3973 0.3048 13.40466482 0.3351 1.39625E-10 1.39625E-07 R2 14000000000 0.3159 0.2234 9.687163595 0.2422 1.73E-11 1.73E-08 R3 480000000 0.2085 0.116 4.782254118 0.1196 2.49167E-10 2.49167E-07 R4 340000000 0.3141 0.2216 9.604958408 0.2401 7.06176E-10 7.06176E-07 R5 470000000 0.2099 0.1174 4.846191486 0.1212 2.57872E-10 2.57872E-07 ug/well glucose Measured' Blanked Glucose Standard Curve 20 0.5483 0.4558 Glucose Standard Curve 16 0.4294 0.3369 Glucose Standard Curve 12 0.3807 0.2882 Glucose Standard Curve 8 0.2872 0.1947 Glucose Standard Curve 4 0.1984 0.1059 Glucose Standard Curve 0 0.0925 0