Cold Tolerance: Inital Study Population: WMA Time point: 1 week post laying No Pretreatment Weigh eggs prior to treatment; if necessary weigh as a group Temperature profile: 6C for 10min; ramps down 0.5C/min until three individuals hit SCP then remove all individuals Post Treatment Fitness Return all eggs to hatching chamber Monitor for hatching; 1 = larvae hatches; 0 = no hatch Units Masses are in grams Temp is in degC Experimental Design: Measure Super cooling point for groups of 6 individuals. Monitor until all individuals freeze. Remove all individuals after this point. Use 3 - 4 sets of 6 eggs for experiment; meaning 18 - 24 eggs total. Check eggs daily for hatching. Record date of hatch if available.