. |-- Cleandata | |-- Day2 | | |-- Day2_1.fq.gz | | |-- Day2_2.fq.gz | | |-- Day2.base.pdf | | |-- Day2.base.png | | |-- Day2.quality.pdf | | `-- Day2.quality.png | |-- Day3 | | |-- Day3_1.fq.gz | | |-- Day3_2.fq.gz | | |-- Day3.base.pdf | | |-- Day3.base.png | | |-- Day3.quality.pdf | | `-- Day3.quality.png | `-- Day5 | |-- Day5_1.fq.gz | |-- Day5_2.fq.gz | |-- Day5.base.pdf | |-- Day5.base.png | |-- Day5.quality.pdf | `-- Day5.quality.png |-- Rawdata | |-- Day2 | | |-- Day2_1.fq.gz | | |-- Day2_2.fq.gz | | |-- Day2.base.pdf | | |-- Day2.base.png | | |-- Day2.quality.pdf | | `-- Day2.quality.png | |-- Day3 | | |-- Day3_1.fq.gz | | |-- Day3_2.fq.gz | | |-- Day3.base.pdf | | |-- Day3.base.png | | |-- Day3.quality.pdf | | `-- Day3.quality.png | `-- Day5 | |-- Day5_1.fq.gz | |-- Day5_2.fq.gz | |-- Day5.base.pdf | |-- Day5.base.png | |-- Day5.quality.pdf | `-- Day5.quality.png *fq.gz: @A201GMABXX:5:1:14057:2058#GATCAG/1 GCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAG + Feffffafffecffffffffeffffceefffcddffeecfcadddddd Format description: Each reads message consists of four lines, the first beginning with '@', followed by a sequence of flags; The third line is the base sequence; The third line begins with a '+', followed optionally by the same sequence of flag information as the first line; The fourth line is the corresponding mass values of the bases in the sequence of the second line, represented by ASCII codes. *base.png: The base positions of the filtered sequence as the abscissa, and the ratio of ATCG bases in each position as the ordinate, the distribution diagram is obtained as follows. In general, the ratio of A base to T base and G base to C base is equal at each sequencing site. *quality.png£º The base position of the filtered sequence is used as the abscissa, and the average mass value of each position is used as the ordinate. Obtaining the sequencing quality distribution map as below. In general, a higher proportion of Q30 sequences accounted, indicating good sequencing quality.