GBS sequence data from eight mouse lemurs (Microcebus tanosi) We analyzed 12 DNA samples from eight individuals of Microcebus tanosi from southern Madagascar. The samples included four pairs of samples each from one individual and four single samples. (Table 1). For details on sample collection, preparation and analysis, see Hapke and Thiele (2015) GIbPSs: a toolkit for fast and accurate analyses of genotyping-by-sequencing data without a reference genome. Submitted to Molecular Ecology Resources. Table 1: Individuals of Microcebus tanosi included in this study Individual Sampling_locality Latitude Longitude Barcode M248 Sainte Luce -24.770804 47.173638 ACTA,GCGT M250 Sainte Luce -24.770804 47.173638 AGGC M316 Manantantely -24.9825 46.9274 TGCGA M319 Manantantely -24.9825 46.9274 ATTGA,TGCAAGGA M341 Petit Lavasoa -25.080894 46.762151 GAGATA,CATAAGT M343 Petit Lavasoa -25.080894 46.762151 TTCAGA M395 Farafara -24.8481 47.0109 GCTCTA M396 Farafara -24.8481 47.0109 GAATTCA,ACGACTAC We constructed a genotyping-by-sequencing library according to the protocol of Elshire et al. (2011) with several modifications. For details see the manuscript. We used BamHI as restriction enzyme. The barcode adapters consisted of the following oligonucleotides: 5’-ACACTCTTTCCCTACACGACGCTCTTCCGATCTxxxx 5’-GATCyyyyAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT with xxxx and yyyy symbolizing one of 12 barcodes (Table S1) and its reverse complement. Sequences of the common adapter were as follows: 5’-GATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 5’-TGACTGGAGTTCAGACGTGTGCTCTTCCGATCT We used the following primers for PCR amplification: 5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT 5’-CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC We used a Pippin Prep (Sage Science) with a 2% Ethidium-free Agarose Gel Cassette to extract molecules with a size range of 150-500 bp from the library. The IMSB NGS unit at Mainz University then performed paired-end sequencing with 101 bp reads of the size-selected fragments on an Illumina HiSeq 2000/2500 system in high output run mode. We obtained 48,236,757 read pairs with a single read length of 101 nucleotides. We then used the programs phredi and phred_pos_filter, which are part of GIbPSs, for an initial quality check and prefiltering of the sequence data. We removed read pairs that had any Phred score below 10 at sequence positions 71-81 in the forward read. After filtering, we retained 32,848,849 Mio read pairs. Files The filtered sequence data are contained in 13 pairs of gzip-compressed fastq files. Paired files have corresponding names. Files with "_R1_" in the filename contain forward reads, which begin with a barcode. Files with "_R2_" in the filename contain reverse reads. Forward and reverse reads appear in corresponding order in the files. The 26 files are contained in six tar archives: M_tanosi_1.tar, M_tanosi_2.tar, M_tanosi_3.tar, M_tanosi_4.tar, M_tanosi_5.tar, M_tanosi_6.tar. Reference Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES, Mitchell SE (2011) A Robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE, 6(5): e19379.