Well Well Type Replicate Threshold Ct Quantity R Sq Slope A1 NTC 1 Reference Reference Reference Reference Reference A1 NTC 1 0.5099 No Ct No Ct 0.996 -3.755 B1 NTC 1 Reference Reference Reference Reference Reference B1 NTC 1 0.5099 No Ct No Ct 0.996 -3.755 C1 Standard 2 Reference Reference Reference Reference Reference C1 Standard 2 0.5099 18.49 3.33e+005 0.996 -3.755 D1 Standard 2 Reference Reference Reference Reference Reference D1 Standard 2 0.5099 18.43 3.33e+005 0.996 -3.755 E1 Standard 3 Reference Reference Reference Reference Reference E1 Standard 3 0.5099 19.53 1.67e+005 0.996 -3.755 F1 Standard 3 Reference Reference Reference Reference Reference F1 Standard 3 0.5099 19.63 1.67e+005 0.996 -3.755 G1 Standard 4 Reference Reference Reference Reference Reference G1 Standard 4 0.5099 20.86 8.35e+004 0.996 -3.755 H1 Standard 4 Reference Reference Reference Reference Reference H1 Standard 4 0.5099 20.97 8.35e+004 0.996 -3.755 A2 Standard 5 Reference Reference Reference Reference Reference A2 Standard 5 0.5099 22.26 4.18e+004 0.996 -3.755 B2 Standard 5 Reference Reference Reference Reference Reference B2 Standard 5 0.5099 22.10 4.18e+004 0.996 -3.755 D2 Standard 6 Reference Reference Reference Reference Reference D2 Standard 6 0.5099 23.11 2.09e+004 0.996 -3.755 E2 Standard 7 Reference Reference Reference Reference Reference E2 Standard 7 0.5099 23.96 1.05e+004 0.996 -3.755 F2 Standard 7 Reference Reference Reference Reference Reference F2 Standard 7 0.5099 24.01 1.05e+004 0.996 -3.755 G2 Standard 8 Reference Reference Reference Reference Reference G2 Standard 8 0.5099 25.32 5.25e+003 0.996 -3.755 H2 Standard 8 Reference Reference Reference Reference Reference H2 Standard 8 0.5099 25.33 5.25e+003 0.996 -3.755 A3 Unknown 9 Reference Reference Reference Reference Reference A3 Unknown 9 0.5099 21.98 4.050e+004 0.996 -3.755 B3 Unknown 10 Reference Reference Reference Reference Reference B3 Unknown 10 0.5099 22.31 3.324e+004 0.996 -3.755 C3 Unknown 11 Reference Reference Reference Reference Reference C3 Unknown 11 0.5099 22.86 2.363e+004 0.996 -3.755 D3 Unknown 12 Reference Reference Reference Reference Reference D3 Unknown 12 0.5099 22.92 2.278e+004 0.996 -3.755 E3 Unknown 13 Reference Reference Reference Reference Reference E3 Unknown 13 0.5099 22.11 3.761e+004 0.996 -3.755 F3 Unknown 14 Reference Reference Reference Reference Reference F3 Unknown 14 0.5099 21.80 4.531e+004 0.996 -3.755 G3 Unknown 15 Reference Reference Reference Reference Reference G3 Unknown 15 0.5099 23.87 1.273e+004 0.996 -3.755 H3 Unknown 16 Reference Reference Reference Reference Reference H3 Unknown 16 0.5099 23.76 1.361e+004 0.996 -3.755 A4 Unknown 17 Reference Reference Reference Reference Reference A4 Unknown 17 0.5099 24.04 1.148e+004 0.996 -3.755 B4 Unknown 18 Reference Reference Reference Reference Reference B4 Unknown 18 0.5099 24.72 7.571e+003 0.996 -3.755 C4 Unknown 19 Reference Reference Reference Reference Reference C4 Unknown 19 0.5099 23.77 1.354e+004 0.996 -3.755 D4 Unknown 20 Reference Reference Reference Reference Reference D4 Unknown 20 0.5099 23.98 1.188e+004 0.996 -3.755 E4 Unknown 21 Reference Reference Reference Reference Reference E4 Unknown 21 0.5099 22.95 2.245e+004 0.996 -3.755 F4 Unknown 22 Reference Reference Reference Reference Reference F4 Unknown 22 0.5099 23.17 1.956e+004 0.996 -3.755 G4 Unknown 23 Reference Reference Reference Reference Reference G4 Unknown 23 0.5099 23.14 1.990e+004 0.996 -3.755 H4 Unknown 24 Reference Reference Reference Reference Reference H4 Unknown 24 0.5099 23.36 1.742e+004 0.996 -3.755 A5 Unknown 25 Reference Reference Reference Reference Reference A5 Unknown 25 0.5099 22.68 2.644e+004 0.996 -3.755 B5 Unknown 26 Reference Reference Reference Reference Reference B5 Unknown 26 0.5099 22.96 2.222e+004 0.996 -3.755 C5 Unknown 27 Reference Reference Reference Reference Reference C5 Unknown 27 0.5099 22.87 2.347e+004 0.996 -3.755 D5 Unknown 28 Reference Reference Reference Reference Reference D5 Unknown 28 0.5099 22.54 2.880e+004 0.996 -3.755 E5 Unknown 29 Reference Reference Reference Reference Reference E5 Unknown 29 0.5099 23.70 1.413e+004 0.996 -3.755 F5 Unknown 30 Reference Reference Reference Reference Reference F5 Unknown 30 0.5099 22.57 2.822e+004 0.996 -3.755 G5 Unknown 31 Reference Reference Reference Reference Reference G5 Unknown 31 0.5099 22.37 3.203e+004 0.996 -3.755 H5 Unknown 32 Reference Reference Reference Reference Reference H5 Unknown 32 0.5099 22.75 2.532e+004 0.996 -3.755 A6 Unknown 33 Reference Reference Reference Reference Reference A6 Unknown 33 0.5099 21.98 4.048e+004 0.996 -3.755 B6 Unknown 34 Reference Reference Reference Reference Reference B6 Unknown 34 0.5099 22.98 2.199e+004 0.996 -3.755 C6 Unknown 35 Reference Reference Reference Reference Reference C6 Unknown 35 0.5099 22.69 2.632e+004 0.996 -3.755 D6 Unknown 36 Reference Reference Reference Reference Reference D6 Unknown 36 0.5099 22.82 2.421e+004 0.996 -3.755 E6 Unknown 37 Reference Reference Reference Reference Reference E6 Unknown 37 0.5099 22.37 3.197e+004 0.996 -3.755 F6 Unknown 38 Reference Reference Reference Reference Reference F6 Unknown 38 0.5099 21.97 4.089e+004 0.996 -3.755 G6 Unknown 39 Reference Reference Reference Reference Reference G6 Unknown 39 0.5099 24.09 1.116e+004 0.996 -3.755 H6 Unknown 40 Reference Reference Reference Reference Reference H6 Unknown 40 0.5099 24.43 9.038e+003 0.996 -3.755 A7 Unknown 41 Reference Reference Reference Reference Reference A7 Unknown 41 0.5099 25.03 6.261e+003 0.996 -3.755 B7 Unknown 42 Reference Reference Reference Reference Reference B7 Unknown 42 0.5099 24.60 8.161e+003 0.996 -3.755 C7 Unknown 43 Reference Reference Reference Reference Reference C7 Unknown 43 0.5099 23.91 1.243e+004 0.996 -3.755 D7 Unknown 44 Reference Reference Reference Reference Reference D7 Unknown 44 0.5099 24.54 8.443e+003 0.996 -3.755 E7 Unknown 45 Reference Reference Reference Reference Reference E7 Unknown 45 0.5099 23.94 1.219e+004 0.996 -3.755 F7 Unknown 46 Reference Reference Reference Reference Reference F7 Unknown 46 0.5099 23.59 1.516e+004 0.996 -3.755 G7 Unknown 47 Reference Reference Reference Reference Reference G7 Unknown 47 0.5099 23.70 1.415e+004 0.996 -3.755 H7 Unknown 48 Reference Reference Reference Reference Reference H7 Unknown 48 0.5099 24.34 9.532e+003 0.996 -3.755 A8 Unknown 49 Reference Reference Reference Reference Reference A8 Unknown 49 0.5099 24.57 8.277e+003 0.996 -3.755 B8 Unknown 50 Reference Reference Reference Reference Reference B8 Unknown 50 0.5099 24.04 1.151e+004 0.996 -3.755 C8 Unknown 51 Reference Reference Reference Reference Reference C8 Unknown 51 0.5099 22.63 2.732e+004 0.996 -3.755 D8 Unknown 52 Reference Reference Reference Reference Reference D8 Unknown 52 0.5099 23.40 1.701e+004 0.996 -3.755 E8 Unknown 53 Reference Reference Reference Reference Reference E8 Unknown 53 0.5099 22.58 2.816e+004 0.996 -3.755 F8 Unknown 54 Reference Reference Reference Reference Reference F8 Unknown 54 0.5099 23.10 2.039e+004 0.996 -3.755 G8 Unknown 55 Reference Reference Reference Reference Reference G8 Unknown 55 0.5099 23.10 2.040e+004 0.996 -3.755 H8 Unknown 56 Reference Reference Reference Reference Reference H8 Unknown 56 0.5099 23.34 1.763e+004 0.996 -3.755 A9 Unknown 57 Reference Reference Reference Reference Reference A9 Unknown 57 0.5099 24.41 9.123e+003 0.996 -3.755 B9 Unknown 58 Reference Reference Reference Reference Reference B9 Unknown 58 0.5099 24.71 7.608e+003 0.996 -3.755 C9 Unknown 59 Reference Reference Reference Reference Reference C9 Unknown 59 0.5099 23.98 1.193e+004 0.996 -3.755 D9 Unknown 60 Reference Reference Reference Reference Reference D9 Unknown 60 0.5099 24.60 8.156e+003 0.996 -3.755 E9 Unknown 61 Reference Reference Reference Reference Reference E9 Unknown 61 0.5099 23.68 1.431e+004 0.996 -3.755 F9 Unknown 62 Reference Reference Reference Reference Reference F9 Unknown 62 0.5099 24.39 9.249e+003 0.996 -3.755 G9 Unknown 63 Reference Reference Reference Reference Reference G9 Unknown 63 0.5099 22.28 3.386e+004 0.996 -3.755 H9 Unknown 64 Reference Reference Reference Reference Reference H9 Unknown 64 0.5099 22.43 3.080e+004 0.996 -3.755 A10 Unknown 65 Reference Reference Reference Reference Reference A10 Unknown 65 0.5099 22.27 3.389e+004 0.996 -3.755 B10 Unknown 66 Reference Reference Reference Reference Reference B10 Unknown 66 0.5099 23.93 1.227e+004 0.996 -3.755 C10 Unknown 67 Reference Reference Reference Reference Reference C10 Unknown 67 0.5099 22.81 2.436e+004 0.996 -3.755 D10 Unknown 68 Reference Reference Reference Reference Reference D10 Unknown 68 0.5099 22.70 2.618e+004 0.996 -3.755 E10 Unknown 69 Reference Reference Reference Reference Reference E10 Unknown 69 0.5099 23.10 2.042e+004 0.996 -3.755 F10 Unknown 70 Reference Reference Reference Reference Reference F10 Unknown 70 0.5099 24.19 1.049e+004 0.996 -3.755 G10 Unknown 71 Reference Reference Reference Reference Reference G10 Unknown 71 0.5099 23.54 1.557e+004 0.996 -3.755 H10 Unknown 72 Reference Reference Reference Reference Reference H10 Unknown 72 0.5099 22.42 3.093e+004 0.996 -3.755 A11 Unknown 73 Reference Reference Reference Reference Reference A11 Unknown 73 0.5099 23.52 1.583e+004 0.996 -3.755 B11 Unknown 74 Reference Reference Reference Reference Reference B11 Unknown 74 0.5099 22.98 2.197e+004 0.996 -3.755 C11 Unknown 75 Reference Reference Reference Reference Reference C11 Unknown 75 0.5099 23.82 1.318e+004 0.996 -3.755 D11 Unknown 76 Reference Reference Reference Reference Reference D11 Unknown 76 0.5099 23.66 1.448e+004 0.996 -3.755 E11 Unknown 77 Reference Reference Reference Reference Reference E11 Unknown 77 0.5099 23.64 1.469e+004 0.996 -3.755 F11 Unknown 78 Reference Reference Reference Reference Reference F11 Unknown 78 0.5099 23.96 1.206e+004 0.996 -3.755 G11 Unknown 79 Reference Reference Reference Reference Reference G11 Unknown 79 0.5099 23.98 1.194e+004 0.996 -3.755 H11 Unknown 80 Reference Reference Reference Reference Reference H11 Unknown 80 0.5099 24.36 9.451e+003 0.996 -3.755