This READ_ME_Behavioral_performance_requirements_for_division_of_labor_influence_adaptive_brain_mosaicism_in_a_socially_complex_ant.txt file was generated on 2021-07-18 by Isabella Muratore GENERAL INFORMATION Title of Dataset: Behavioral performance requirements for division of labor influence adaptive brain mosaicism in a socially complex ant Author Information A. Corresponding Author Contact Information Name: Isabella Muratore Institution: Boston University Email: isabella.b.muratore@gmail.com B. Associate Author Contact Information Name: Eva Fandozzi Institution: Boston University Email: edozzi95@gmail.com C. Associate Author Contact Information Name: James Traniello Institution: Boston University Email: jft@bu.edu Author contributions to data collection and analysis: IBM and JFAT designed the study. IM drafted the manuscript. IM, EMF, and JFAT developed and edited the manuscript. IBM and EMF prepared and imaged brains. EMF measured neuropils and IBM and EMF statistically analyzed volumetric data. JFAT secured funding. Funding sources that supported the collection of data: This study was funded by Mational Science Foundation grants IOS 1354291 and IOS 1953393 to JFAT, a Boston University Undergraduate Research Opportunities Program award to EMF, and the Department of Biology Brenton R. Lutz Award and Belamarich Dissertation Writing Award to IBM. SHARING/ACCESS INFORMATION Please do not use this data set for publication without consulting the corresponding author. DATA & FILE OVERVIEW File List: Table 1 Tasks performed by A. cephalotes workers.csv Tasks performed by A. cephalotes workers, their relative reliance on sensory modalities, sensory input processing, high-order integration, and motor coordination in relation to division of labor. Sensory and sensorimotor inputs of tasks and processing are rated on a 0-3 scale depending on the role an input or process is considered to serve in a given task. 0 = not involved, 1 = possible role, 2 = likely role, and 3 = significant role. The same scale was used to rate the performance frequency of a given task in the repertoire of different-size workers. The “proprioception, somatosensory processing and kinesthesis” category refers to requirements above common body and appendage motor control systems across polymorphic workers that reflect greater kinesthetic coordination than standing, walking without load carriage, feeding, mouthpart and appendage movement, self-grooming, and allogrooming. Value assignments are based on published results and personal observations. Supplementary Table 1 Absolute volumes.csv Absolute volumes (µm3) of brain compartments and sums. Bin indicates HW(mm) category, divided into three groups (bins) for media workers. HW indicates actual size measurements of sampled individuals according to bin grouping. Supplementary Table 2 Proportional volumes.csv Proportional volumes of brain compartments as percent of total brain tissue measured for each individual. Bin indicates HW (mm) category, divided into three groups (bins) for media workers. HW indicates actual size measurements of sampled individuals according to bin grouping. Supplementary Table 4 LDA training set.csv Normalized A. cephalotes brain volume measurements (annotated by a different observer from that used to annotate brain images in main data set) used for linear discriminant analysis training. Bin indicates HW (mm) category, divided into three groups (bins) for media workers. HW indicates actual size measurements of sampled individuals according to bin grouping. METHODOLOGICAL INFORMATION Methods used for collection of data: Mature fully sclerotized workers collected from colonies Ac09, Ac16, Ac20, and Ac21 were decapitated immediately prior to brain dissection and fixation. Workers were sampled from five worker size groups identified by head width (HW): minims (0.6mm±0.1mm), medias (1.2mm±0.1mm, 1.8±0.1mm, or 2.4mm±0.1mm), and majors (3.0mm or larger). Brains (n=30) from workers sampled from Ac09, Ac20, and Ac21 were dissected in ice-cold HEPES Buffered Saline (HBS), placed in 16% zinc-formaldehyde (Ott 2008) and fixed overnight at room temperature (RT) on a shaker. Whole brains were processed to visualize the presynaptic protein synapsin. Fixed brains were washed in HBS six times, 10 minutes per wash, and fixed in Dent’s Fixative (80% MeOH, 20% DMSO) for minimally 1 hour. Brains were then washed in 100% methanol and either stored at -17°C or immediately processed. Brains were washed in 0.1M Tris buffer (pH=7.4) and blocked in PBSTN (5% neutral goat serum, 0.005% sodium azide in 0.2% PBST) at RT for 1 hour before incubation for 3 days at RT in primary antibody (1:30 SYNORF 1 in PBSTN; monoclonal antibody anti-synorf 3C11 obtained from DSHB, University of Iowa, IA, USA; 62). They were washed 6x10 minutes in 0.2% PBST and incubated in the secondary antibody (1:100 AlexaFluor 488 goat anti-mouse in PBSTN) for 4 days at RT. Brains were then washed a final time (6x10 minutes in 0.2% PBST) and dehydrated in an ethanol and PBS series (10 minutes per concentration, 30/50/70/95/100/100% ethanol in 1x PBS), then cleared with and immersed in methyl salicylate, and mounted on stainless steel glass windowed slides for imaging. Methods for processing data: Brains were imaged with a Nikon C2 confocal microscope and images were manually annotated using Amira 6.0 software to quantify neuropil volumes (not including cell bodies). We recorded the volumes of OL, AL, MB, CX, SEZ, and ROCB. We also measured substructures of the MB: the medial calyxes (MB-MC), lateral calyxes (MB-LC), and peduncle and lobes (MB-P). These were combined to quantify total MB size (MB-S) across worker size groups. For bilateral structures, one hemisphere was measured, and for compartments located along the brain midline (SEZ and CX), the whole structure was measured. DATA-SPECIFIC INFORMATION FOR: Table 1 Tasks performed by A. cephalotes workers.csv Variable List: Behavioral performance/sensory/cognitive process: Vision, Olfaction, Gustation; Contact chemo-reception, Seismic signal perception, Anemotaxis; air current mechanoreception, Magneto-reception, Thermo-reception, Hygro-reception, Proprioception; Somatosensory processing; Kinesthesis, Sensory integration Size (HW, mm): 0.6, 1.2, 1.8, 2.4, >=3 Ref. (i.e. supporting references) DATA-SPECIFIC INFORMATION FOR: Supplementary Table 1 Absolute volumes.csv Variable List: Colony: Ac09, Ac16, Ac20, and Ac21 ID (i.e. individual brain sample ID) Bin: 0.6, 1.2, 1.8, 2.4, 3 SUM (i.e. sum of all measured neuropils) OL (i.e. optic lobe) AL (i.e. antennal lobe) MB-MC (i.e. mushroom body medial calyces) MB-LC (i.e. mushroom body lateral calyces) MB-P (i.e. mushroom body peduncle) CX (i.e. central complex) SEZ (i.e. subesophageal zone) ROCB (i.e. rest of central brain) DATA-SPECIFIC INFORMATION FOR: Supplementary Table 2 Proportional volumes.csv Variable List: Colony: Ac09, Ac16, Ac20, and Ac21 ID (i.e. individual brain sample ID) Bin: 0.6, 1.2, 1.8, 2.4, 3 OL (i.e. optic lobe) AL (i.e. antennal lobe) MB-MC (i.e. mushroom body medial calyces) MB-LC (i.e. mushroom body lateral calyces) MB-P (i.e. mushroom body peduncle) CX (i.e. central complex) SEZ (i.e. subesophageal zone) ROCB (i.e. rest of central brain) MB-S (i.e. mushroom body total sum) DATA-SPECIFIC INFORMATION FOR: Supplementary Table 4 LDA training set.csv Variable List: Colony: Ac09, Ac16, Ac20, and Ac21 ID (i.e. individual brain sample ID) Bin: 0.6, 1.2, 1.8, 2.4, 3 OL (i.e. optic lobe) AL (i.e. antennal lobe) MB-MC (i.e. mushroom body medial calyces) MB-LC (i.e. mushroom body lateral calyces) MB-P (i.e. mushroom body peduncle) CX (i.e. central complex) SEZ (i.e. subesophageal zone) ROCB (i.e. rest of central brain)