This Gasmi_PKF_readme.txt file was generated on 2021-05-11 by Cathy Coutu. GENERAL INFORMATION 1. Title of Dataset: A horizontally transmitted Parasitoid Killing Factor protects insects from parasitoids 2. Author Information A. Principal Investigator Contact Information Name: Martin Erlandson Institution: Agriculture and Agri-Food Canada Address: 107 Science Place, Saskatoon, SK, S7N 0X2, Canada Email: martin.erlandson@canada.ca B. Associate or Co-investigator Contact Information Name: Salvador Herrero Institution: Department of Genetics and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI-BIOTECMED) Address: Universitat de València 46100 – Burjassot (Valencia), Spain Email: salvador.herrero@uv.es C. Alternate Contact Information Name: Madoka Nakai Institution: Tokyo University of Agriculture and Technology Address: Fuchu, Tokyo, 183-8509, Japan Email: madoka@cc.tuat.ac.jp 3. Date of data collection (single date, range, approximate date): 2018-2019 4. Information about funding sources that supported the collection of the data: Laila Gasmi was awarded a post-doctoral fellowship from Japanese Society for the Promotion of Science, JSPS (PE16037). This work was supported by JSPS Grant in Aid (14360200, 24380035 and 15K14894), partially supported by a grant (No. 2017R1A2133009815) of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea, in addition to Agriculture and Agri-Food Canada grants J-001033 and J-000778. Research at the University of Valencia is supported by project from the Spanish Ministry of Science, Innovation and Universities (No. AGL2014‐57752‐C2‐2R, RTI2018-094350-B-C32). SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: None 2. Links to publications that cite or use the data: Science, article number abb6396 3. Links to other publicly accessible locations of the data: Accession_numbers.txt contains the GenBank Accession numbers for all the sequences 4. Links/relationships to ancillary data sets: None 5. Was data derived from another source? No A. If yes, list source(s): 6. Recommended citation for this dataset: Gasmi et. al. (2021). A horizontally transmitted Parasitoid Killing Factor protects insects from parasitoids. Science, in press. DATA & FILE OVERVIEW 1. File List: Accession_numbers.txt : a Tab-delimited file containing the PKF name, organism, and GenBank Accession number. Figure2a_PKF_alignment.txt: ClustalW format alignment of the amino acid sequences of the Parasitoid Killing Factors in this study. Figure2a_PKF_tree.txt: NWK format tree based on PKR_alignment.txt PKF_Domain_Alignment_for_GLAM2.txt: ClustalW format alignment of the repeated N-terminal domain, used by GLAM2 to generate a PSSM. Supplemental_figure_S2_PKF_alignment.txt: ClustalW format alignment of the amino acid sequences of select Parasitoid Killing Factors, highlighting the repeated N-terminal domains. 2. Relationship between files, if important: PKF_alignment.txt was used to generate PKF_tree.txt. METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: PKF homologs from dsDNA viruses and noctuid insects were obtained by Blastp and PSI-Blast searches of the NCBI protein database or BLASTX searches of all noctuid genomes in NCBI. Genomic contigs containing the PKF homologs in the different host species were retrieved from NCBI or the specific genomic projects and manually annotated using Geneious software (35). The identity of annotated ORFs flanking the different pkf homologs was manually assessed by Blastp and Blastx searches against the NCBI nr protein database. 2. Methods for processing the data: The predicted amino acid sequences were aligned using the ClustalW2 or ClustalO algorithm in CLC Genomic Workbench v10 or in MEGA7 using the WAG δ distribution model. Evolutionary distance was calculated for aligned sequences by the Maximum-likelihood method and the phylogenetic trees were constructed with CLC Genomic Workbench v10 and MEGA7 programs. The reliability of inferred trees was determined using a bootstrap test (100 replicates). Phylogenetic tree images were created in iTOL v3: Interactive Tree of Life web server (www. itol.embl.de) GLAM2 (http://meme-suite.org/doc/glam2.html), was used to identify novel gapped motifs and generate position-specific scoring matrices (PSSMs) to represent specific motifs. GLAM2SCAN was used to search mulitfasta sequence files for those PSSMs. The PSSM logo platform was used to visualize the PSSMs for readability (37). DATA-SPECIFIC INFORMATION FOR: Accession_numbers.txt column 1: name of the PKF column 2: species PKF was found in column 3: Accession number of the sequence DATA-SPECIFIC INFORMATION FOR: Figure2a_PKF_alignment.txt Alignment of the amino acid sequences for 56 viral and insect Parasitoid Killing Factors. ClustalW format. DATA-SPECIFIC INFORMATION FOR: Figure2a_PKF_tree.txt Alignment tree for 56 viral and insect Parasitoid Killing Factors. Newick Format. DATA-SPECIFIC INFORMATION FOR: PKF_Domain_Alignment_for_GLAM2.txt Alignment of the amino acid sequences for the repeated N-terminal domain found in Parasitoid Killing Factors. ClustalW Format. DATA-SPECIFIC INFORMATION FOR: Supplemental_figure_S2_PKF_alignment.txt Alignment of the amino acid sequences for 47 viral and insect Parasitoid Killing Factors. ClustalW format.