The sequencing data obtained from Novogene for all strains was analyzed using breseq. The reference sequences were GENOME_SIJ488.fasta and pZ-ASS-alaA.fasta . The sequencing results for the strains are as follows: Glutaux +alaA mutant 1 files: D14_FDSW202507757-1r_HCK2GDSXY_L4_2.fasta D14_FDSW202507757-1r_HHGV7DSXY_L2_2.fasta Glutaux +alaA mutant 2 files: D15_FDSW202507757-1r_HCK2GDSXY_L4_2.fasta D15_FDSW202507757-1r_HHGV7DSXY_L2_2.fasta Glutaux +alaA parent files: D19_FDSW202507762-1r_HCHWNDSXY_L3_2.fasta D19_FDSW202507762-1r_HHGV7DSXY_L1_2.fasta Differences between parent Glutaux +alaA parent, and Glutaux +alaA mutant 1 or Glutaux +alaA mutant 2 , respectively, were reported as mutations in the manuscript.