This file contains all the raw data used in Mridha S and KŸmmerli R (2022) Journal of Evolutionary Biology Paper title: Enforced specialization fosters mutual cheating and not division of labour in the bacterium Pseudomonas aeruginosa Sheet : Fig1a variables Time Interval of measurements (every 15 minutes) Mix Percentage of PVD (pyoverdine), PCH (pyochelin) producers or wildtype mean.OD600 Average growth (OD600) across replicates for a particular treatment (unique Time, Mix and Media combination) SD.OD600 Standard deviation of growth (OD600) across replicates for a particular treatment mean.Normalised_mCherry Average mCherry fluorescence after substracting the mean untagged wildtype mCherry fluorescence for a particular Time and Media SD.Normalised_mCherry Standard deviation of mCherry fluorescence after substracting the mean untagged wildtype mCherry fluorescence for a particular Time and Media mean.Normalised_GFP Average GFP fluorescence after substracting the mean untagged wildtype GFP fluorescence for a particular Time and Media SD.Normalised_GFP Standard deviation of GFP fluorescence after substracting the mean untagged wildtype GFP fluorescence for a particular Time and Media Media Media conditions (iron limited or iron rich) Sheet : Fig1b variables Mix Percentage of PVD (pyoverdine), PCH (pyochelin) producers or wildtype Replicate Replicates Experiment Biological repeats Integral.OD600 Productivity or growth measured as area under the OD600 curve over 24 hours MaxSlope_Growthrate Growthrate (OD600/time) OD600_endpoint OD600 measured after 24 hours Lamba_Lagphase Duration between experiment start and beginning of exponential phase Peak.OD600 Highest OD600 measurement Media Media conditions (iron limited or iron rich) Sheet : Fig2 variables Mix Theoretical percentage of PVD (pyoverdine) & PCH (pyochelin) producers in a mix Replicate Replicates Experiment Biological repeats Integral.OD600 Productivity or growth measured as area under the OD600 curve over 24 hours Media Media conditions (iron limited or iron rich) rel_Integral Growth integral of different mixes divided by wildtype growth integral for a particular media Pyochelin_prod Theoretical percentage of pyochelin producers in a mix Sheet : Fig3 variables Media Media conditions (iron limited or iron rich) avg_log_Rel_GFP log transformed average relative fitness of GFP over mCherry tagged strain producing the same siderophore avg_log_Rel_mch log transformed average relative fitness of mCherry over GFP tagged strain producing the same siderophore Mix Theoretical percentage of PVD (pyoverdine) & PCH (pyochelin) producers in a mix GFP_percent_t0 Actual percentage of GFP (pyochelin) producing strain quantified with flowcytometer at start (time = 0 hours) mch_percent_t0 Actual percentage of mCherry (pyoverdine) producing strain quantified with flowcytometer at start (time = 0 hours) GFP_percent_t24 Actual percentage of GFP (pyochelin) producing strain quantified with flowcytometer in the end (time = 24 hours) mch_percent_t24 Actual percentage of mCherry (pyoverdine) producing strain quantified with flowcytometer in the end (time = 24 hours) Experiment Experiment number Replicate Replicates log_Rel_PCH_GFP log transformed relative fitness of pyochelin (GFP) producing strain log_Rel_PVD_mch log transformed relative fitness of pyoverdine (mCherry) producing strain net_log_Rel_PCH_GFP log transformed relative fitness of pyochelin (GFP) producing strain after substracting "avg_log_Rel_GFP" net_log_Rel_PVD_mch log transformed relative fitness of pyoverdine (mCherry) producing strain after substracting "avg_log_Rel_mch" Pyochelin_prod Theoretical percentage of pyochelin producers in a mix Sheet : FigS2 variables Mix Theoretical percentage of strains producing identical siderophore, tagged either with GFP and mCherry Date Date of the experiment Media Media conditions (iron limited or iron rich) Siderophore Siderophore produced (pyochelin or pyoverdine) log_Rel_GFP log transformed relative fitness of the GFP producing strain Sheet : FigS3a variables Mix Theoretical percentage of PVD (pyoverdine) & PCH (pyochelin) producers in a mix Replicate Replicates Experiment Experiment number Media Media conditions (iron limited or iron rich) Pyochelin_prod Theoretical percentage of pyochelin producers in a mix GFP_percent_t0 Actual percentage of GFP (pyochelin) producing strain quantified with flowcytometer at start (time = 0 hours) GFP_percent_t24 Percentage of GFP (pyochelin) producing strain in the end (time = 24 hours) compare GFP (pyochelin) producing strain in the end (time = 24 hours) either observed (quantified with flow cytometer) or expected (based on monoculture growth) Sheet : FigS3b variables Mix Theoretical percentage of PVD (pyoverdine) & PCH (pyochelin) producers in a mix Replicate Replicates Experiment Experiment number Media Media conditions (iron limited or iron rich) Pyochelin_prod Theoretical percentage of pyochelin producers in a mix mch_percent_t0 Actual percentage of mCherry (pyoverdine) producing strain quantified with flowcytometer at start (time = 0 hours) mch_percent_t24 Percentage of mCherry (pyoverdine) producing strain in the end (time = 24 hours) compare mCherry (pyoverdine) producing strain in the end (time = 24 hours) either observed (quantified with flow cytometer) or expected (based on monoculture growth)