This DATASET_Mancuso_2021_readme.txt file was generated on 2022—01-28 by Francesca Mancuso GENERAL INFORMATION 1. Title of Dataset: EFFECTS OF TITANIUM DIOXIDE NANOPARTICLES ON PORCINE PRE-PUBERTAL SERTOLI CELLS 2. Author Information A. Principal and Corresponding Investigator Contact Information Name: Francesca Mancuso Institution: University of Perugia Address: Piazzale L. Severi Email: francesca.mancuso@unipg.it B. Principal investigator Contact Information Name: Iva Arato Institution: University of Perugia Address: Piazzale L. Severi Email: iva.arato@libero.it C. Principal investigator Contact Information Name: Alessandro Di Michele Institution: University of Perugia Address: Via del Liceo,1 Email: alessandro.dimichele@unipg.it. D, Co-investigator Contact Information Name: Cinzia Antognelli Institution: University of Perugia Address: Piazzale L. Severi Email: cinzia.antognelli@unipg.it E, Co-investigator Contact Information Name: Luca Angelini Institution: University of Perugia Address: Piazzale L. Severi Email: angeluca91@hotmail.it F, Co-investigator Contact Information Name: Catia Bellucci Institution: University of Perugia Address: Piazzale L. Severi Email: catia.bellucci@unipg.it G, Co-investigator Contact Information Name: Cinzia Lilli Institution: University of Perugia Address: Piazzale L. Severi Email: cinzia.lilli@unipg.it H. Co-investigator Contact Information Name: Simona Boncompagni Institution: University of Chieti-Pescara Address: Via dei Vestini, 31 Email: simona.boncompagni@unich.it. I. Co-investigator Contact Information Name: Aurora Fusella Institution: University of Chieti-Pescara Address: Via dei Vestini, 31 Email: aurora.fusella@unich.it. J, Co-investigator Contact Information Name: Desirèe Bartolini Institution: University of Perugia Address: Piazzale L. Severi Email: desirex85@hotmail.it, K, Co-investigator Contact Information Name: Carla Russo Institution: University of Perugia Address: Piazzale L. Severi Email: carla.russo179@gmail.com L, Co-investigator Contact Information Name: Massimo Moretti Institution: University of Perugia Address: Piazzale L. Severi Email: massimo.moretti@unipg.it M, Co-investigator Contact Information Name: Morena Nocchetti Institution: University of Perugia Address: Piazzale L. Severi Email: morena.nocchetti@unipg.it N. Co-investigator Contact Information Name: Angela Gambelunghe Institution: University of Perugia Address: Piazzale L. Severi Email: angela.gambelunghe@unipg.it O. Co-investigator Contact Information Name: Giacomo Muzi Institution: University of Perugia Address: Piazzale L. Severi Email: giacomo.muzi@unipg.it P. Co-investigator Contact Information Name: Tiziano Baroni Institution: University of Perugia Address: Piazzale L. Severi Email: tiziano.baroni@unipg.it Q. Senior and last-authorship Contact Information Name: Stefano Giovagnoli Institution: University of Perugia Address: Piazzale L. Severi Email: stefano.giovagnoli@unipg.it R. Senior and last-authorship Contact Information Name: Giovanni Luca Institution: University of Perugia Address: Piazzale L. Severi Email: giovanni.luca@unipg.it 3. Date of data collection 2019-2021: 4. Geographic location of data collection University of Perugia, Perugia, Italy and University of Chieti-Pescara, Chieti-Pescara, Italy. 5. Information about funding sources that supported the collection of the data: Fondazione Cassa di Risparmio di Perugia, code of Project: 2019.0382.029. SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: N/A; not applicable 2. Links to publications that cite or use the data: N/A; not applicable 3. Links to other publicly accessible locations of the data: N/A; not applicable 4. Links/relationships to ancillary data sets: N/A; not applicable 5. Was data derived from another source? No A. If yes, list source(s): 6. Recommended citation for this dataset: Mancuso, Arato et al. (2022), Data from: EFFECTS OF TITANIUM DIOXIDE NANOPARTICLES ON PORCINE PRE-PUBERTAL SERTOLI CELLS, Dryad, Dataset. DATA & FILE OVERVIEW 1. Description of dataset The data were generated to describe the X-ray diffraction (XRD), scanning electron microscopy (SEM) that, through a non-destructive technique, allowed a morphological evaluation and measurement investigation of titanium dioxide nanoparticles (TiO2 NPs) and the transmission electron microscopy (TEM) analysis to investigate the morphological/ultrastructural effects on porcine pre-pubertal Sertoli cells (SCs), after an acute (24 hours) and chronic (from 1 up to 3 weeks) exposure, at both sub-toxic (5 µg/ml) and toxic (100 µg/ml) doses of TiO2 NPs. The diffractogram confirmed the correct synthesis of TiO2 NPs in accordance with the International Centre for Diffraction Data Sample (JCPDS 00-001-0562) and the asterisk (*) represents the peaks of TiO2 NPs in anatase form; SEM analysis showed that the mean size distribution of TiO2 NPs in dry form was 20 ± 5 nm and confirmed that TiO2 NPs tend to form aggregates of submicrometric dimensions. Representative TEM images of unexposed SCs showed elongated oval or spindle-shaped SCs containing quite large nuclei (N) and abundant mitochondria. SCs did not show substantial and/or severe ultrastructural alterations in SCs treated with 5 µg/ml TiO2 NPs for 1 and 2 weeks, in all the other exposure conditions (5 µg/ml for 3 weeks and 100 µg/ml for 1, 2, and 3 weeks), SCs showed several ultrastructural changes with a high percentage of severely damaged SCs, presenting different features of cell stress such as deeply invaginated and shrunk nuclei, disorder/ marginalization of chromatin components, swollen very fragmented, or almost completely missing endoplasmic reticulum membranes. In addition, mitochondrial morphology was severely affected and mitochondria number dramatically decreased with the presence of several large vacuoles probably as a result of apoptotic mitochondria and/or enlarged endoplasmic reticulum and/or increased frequency of lipid droplets. Main results of the experiments are that long time constant exposure of SCs at both TiO2 NPs concentrations inevitably led to an altered ultrastructure. 2. File List: File 1 Name: Mancuso_2022_1_XRD_IDs.tif File 1 Description: X-ray diffraction of TiO2 NPs File 2 Name: Mancuso_2022_2a_SEM_IDs.tif File 2 Description: scanning electron microscopy of TiO2 NPS File 2 Name: Mancuso_2022_2b_SEM_IDs.tif File 2 Description: scanning electron microscopy of TiO2 NPS File 3 Name: Mancuso_2022_3a_TEM_IDs.tif File 3 Description: unexposed SCS 1 week File 4 Name: Mancuso_2022_3b_TEM_IDs.tif File 4 Description: SCs exposed to 5 µg/ml of TiO2 NPs 1 week File 5 Name: Mancuso_2022_3c_TEM_IDs.tif File 5 Description: SCs exposed to 100 µg/ml of TiO2 NPs 1 week File 6 Name: Mancuso_2022_3d_TEM_IDs.tif File 6 Description: unexposed SCS 2 week File 7 Name: Mancuso_2022_3e_TEM_IDs.tif File 7 Description: SCs exposed to 5 µg/ml of TiO2 NPs 2 week File 8 Name: Mancuso_2022_3f_TEM_IDs.tif File 8 Description: SCs exposed to 100 µg/ml of TiO2 NPs 2 week File 9 Name: Mancuso_2022_3g_TEM_IDs.tif File 9 Description: unexposed SCS 3 week File 10 Name: Mancuso_2022_3h_TEM_IDs.tif File 10 Description: SCs exposed to 5 µg/ml of TiO2 NPs 3 week File 11 Name: Mancuso_2022_3i_TEM_IDs.tif File 11 Description: SCs exposed to 100 µg/ml of TiO2 NPs 3 week METHODOLOGICAL INFORMATION The synthesis of the nanoparticles was performed by sonochemical method using acoustic cavitation  at the Laboratory of Acoustic Cavitation of the Department of Physics and Geology of the University of Perugia.  XRD was evaluated at room temperature with the diffractometer Philips X'Pert PRO MPD (Malvern Panalytical Ltd., Royston, UK) operating at 40kW/40 mA with step size of 0.017° and a step scan of 70s, using a Cu Kα radiation and X-Celerator detector. SEM analysis was performed using a LEO 1525 Field Emission Scanning Electron Microscope, ZEISS (Jena, Germany) at the University of Perugia. TEM analysis was performed using a Morgagni Series 268D electron microscope (FEI Company, Brno, Czech Republic) equipped with a Megaview III digital camera and Analy-SIS software (Olympus Soft Imaging Solutions) at the University of Chieti-Pescara. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_1_XRD_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: The asterisk (*) represents the peaks of TiO2 NPs in anatase form 4. Missing data codes: None 5. Specialized formats or other abbreviations used: International Centre for Diffraction Data Sample (JCPDS 00-001-0562) DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_2a_SEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: The green scale bar corresponds to 100 µm. Electron high tension (HET)=15.00 KW Working distance (WD)=8.2 mm Magnification (Mag)=250.00 K X DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_2b_SEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: The green scale bar corresponds to 2 µm. Electron high tension (HET)=15.00 KW Working distance (WD)=8.4 mm Magnification (Mag)=10.00 K X DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3a_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) The scale bar corresponds to 5 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3b_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) Red circles indicate TiO2 NPs deposition. The scale bar corresponds to 1 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3c_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) Asterisks indicate TiO2 NPs deposition. Small red arrows indicate nuclear membrane invaginations The scale bar corresponds to 2 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3d_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) The scale bar corresponds to 5 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3e_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) Red circles indicate TiO2 NPs deposition. The scale bar corresponds to 2 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3f_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) Asterisks indicate TiO2 NPs deposition. Small red arrows indicate nuclear membrane invaginations. Black arrows point to chromatin marginalization. The scale bar corresponds to 2 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3g_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) Asterisks indicate TiO2 NPs deposition. Small red arrows indicate nuclear membrane invaginations. Black arrows point to chromatin marginalization. The scale bar corresponds to 2 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3h_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Nucleus (N) Red circles indicate TiO2 NPs deposition. Large red arrows indicate enlarged endoplasmic reticulum. The scale bar corresponds to 2 µm. DATA-SPECIFIC INFORMATION FOR: Mancuso_2022_3i_TEM_IDs.tif 1. Number of variables: None 2. Number of cases/rows: 1 3. Variable List: 4. Missing data codes: None 5. Specialized formats or other abbreviations used: Asterisks indicate TiO2 NPs deposition. Small red arrows indicate nuclear membrane invaginations. Black arrows point to chromatin marginalization. The scale bar corresponds to 2 µm.