This README file was generated on 2022-05-11 by Ana Angela Romero Haro GENERAL INFORMATION 1. Title of Dataset: Data from: Inter-generational costs of oxidative stress: reduced fitness in daughters of mothers that experienced high levels of oxidative damage during reproduction 2. Author Information A. Corresponding Investigator Name: Dr Ana Angela Romero Haro Institution: University of Exeter Address: Centre for Ecology and Conservation, University of Exeter, Penryn TR10 9FE, United Kingdom Email: a.romero-haro@exeter.ac.uk B. Co-investigator 1 Name: Dr Lorenzo Perez Rodriguez Institution: Instituto de Investigación en Recursos Cinegéticos (IREC) Address: Ronda de Toledo 12, 13005 Ciudad Real, Spain C. Co-investigator 2 Name: Barbara Tschirren Institution: University of Exeter Address: Centre for Ecology and Conservation, University of Exeter, Penryn TR10 9FE, United Kingdom 3. Date of data collection: 2017-2018 4. Geographic location of data collection: Zurich, Switzerland 5. Information about funding sources that supported the collection of the data: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung PP00P3_128386 and PP00P3_157455 6. Recommended citation for this dataset: Romero-Haro et al. 2022. Data from: Inter-generational costs of oxidative stress: reduced fitness in daughters of mothers that experienced high levels of oxidative damage during reproduction DATA & FILE OVERVIEW 1. File List: These data were generated to investigate the intergenerational fitness cost of the parental oxidative damage during reproduction in females Japanese quail (Coturnix japonica). There are two csv files: Romero-Haro_et_al_data. Data for the main text analyses and Romero-Haro_et_al_supplementary. Data for the supplementary analyses. METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: Breeding and husbandry protocol is explained in Pick et al 2016 (Heredity 116:542–549), laboratory protocol (plasma lipid oxidative damage, malondialdehyde acid, MDA) is explained in Romero-Haro and Alonso Alvarez 2014 (Physiol Biochem Zool 87:353–362). Both are also explained in the methods section of the manuscript. Breeding conditions: breeding cages (122 x 50 x 50 cm), 16:8 light : dark cycle at approximately 20 °C. Eggs were collected on the day they were laid, weighed (to the nearest 0.01g), and artificially incubated (Favorit, HEKA Brutgeräte). During the first 14 days, eggs were maintained at 37.8 °C and 55% humidity. They were then transferred to a hatcher (Favorit, HEKA Brutgeräte) and kept at 37.6 °C and 80% humidity until hatching. Chicks were kept in a heated cage (109 × 57 × 25 cm, Kükenaufzuchtbox Nr 4002/C, HEKA Brutgeräte) for two weeks after hatching. The first five days the temperature was kept at 35–38 °C, then slowly lowered to 25 °C over the next 9 days. After two weeks, chicks were transferred to rearing cages within the breeding facility. At the age of four weeks, the birds were released into the outdoor aviaries. Body mass was measured at hatching (to the nearest 0.01g) and at adulthood (i.e. when six months old; to the nearest 1g). Laboratory analyses: A standard curve for calibration was prepared using a 1,1,3,3-tetraethoxypropane stock solution (5 µM in 40% ethanol), serially diluted using 40% ethanol. 50 µL of a butylated hydroxytoluene (BHT) solution (0.05% w/v in 95% ethanol), 400 µL of a phosphoric acid solution (0.44 M), and 100 µL of a thiobarbituric acid (TBA) solution (42 mM) were added to 20 µL of plasma and 30µL of Milli-Q water or to 50 µL of standard, vortexed and heated at 100ºC for 1 h to allow for the formation of MDA-TBA adducts. The reaction was stopped by placing samples and standards on ice. 250 µL of n-butanol was then added to extract the MDA-TBA complex. Tubes were subsequently vortexed and centrifuged at 18 000 g for 3 min at 4ºC. 100 µL of the upper (n-butanol) phase were then moved to HPLC vials, which were immediately saturated with N2 to avoid oxidation (see also Romero-Haro and Alonso-Alvarez 2014). Samples were injected into an Agilent 1100 series HPLC system (Agilent, Waldbronn, Germany) fitted with a fluorescence detector set and a 5-µm ODS-2 C-18 4.0 x 250-mm column maintained at 37ºC. The mobile phase was MeOH : KH2PO4 (50 mM; 40 : 60 v/v), running isocratically for 10 min at a flow rate of 1 mL/min. Chromatograms were collected at 515 nm (excitation) and 553 nm (emission). DATA-SPECIFIC INFORMATION FOR: Romero-Haro_et_al_data 1. Number of variables: 20 2. Number of cases/rows: 22 3. Variable List: Focalfemale: ID of the focal female logMDA.focalfemale: Levels of lipid oxidative damage in plasma (malondialdehyde, MDA), log transformed (units: log (µM)) of the focal female eggs.laid: number of eggs laid by the focal female during 16 days of breeding conditions eggs.not.laid: number of eggs not laid by the focal female during 16 days of breeding conditions. Hatched: number of laid eggs that hatched. Unhatched: number of laid eggs that did not hatch. hatching.success: Hatching success offspringd1: Number of 1 day-old offspring lifespan: number of days of life of focal females cause.death: cause of death of the focal female egg.mass: mass of the egg where the focal female developed in. Grams hatching.mass: hatching body mass of the focal female. Grams adult.mass: adult body mass of the focal female. Grams mother: ID of the mother father: ID of the father logMDA.mother: Levels of lipid oxidative damage in plasma (malondialdehyde, MDA), log transformed (units: log (µM)) of the mother logMDA.father: Levels of lipid oxidative damage in plasma (malondialdehyde, MDA), log transformed (units: log (µM)) of the father stdmother: standardized levels of MDA of the mother stdfather: standardized levels of MDA of the father stdfocal: standardized levels of MDA of the focal female 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: MDA: plasma lipid oxidative damage (malondialdehyde acid) DATA-SPECIFIC INFORMATION FOR: Romero-Haro_et_al_supplementary 1. Number of variables: 24 2. Number of cases/rows: 58 3. Variable List: Group: analysis group (Father, Mother or Focal female). Logmda: Levels of lipid oxidative damage in plasma (malondialdehyde, MDA), log transformed (units: log (µM)) of the individual. Triglicerides: Plasma triglyceride levels of the individual, units: mg/dL mda.resid: MDA levels corrected by the plasma levels of triglycerides. Models explained in the supplementary material focalfemale_ID: ID of the focal female offspringd1: Number of 1 day-old offspring logMDA_focalfemale: Levels of lipid oxidative damage in plasma (malondialdehyde, MDA), log transformed (units: log (µM)) of the focal female eggs.laid: number of eggs laid by the focal female during 16 days of breeding conditions eggs.not.laid: number of eggs not laid by the focal female during 16 days of breeding conditions. duration.breeding.event: duration of the breeding event in days. eggs.hatched_focalfemale: number of laid eggs by the focal female that hatched eggs.unhatched_focalfemale: number of laid eggs by the focal female that did not hatch hatchingsucess: Hatching success lifespan_focalfemale: number of days of life of focal females cause.death: cause of death of the focal female egg.mass.origin_focalfemale: mass of the egg where the focal female developed in. Grams hatching.mass_focalfemale: hatching body mass of the focal female. Grams adult.mass_focalfemale: adult body mass of the focal female. Grams mother_ID: ID of the mother father_ID: ID of the father logMDA.mother: Levels of lipid oxidative damage in plasma (malondialdehyde, MDA), log transformed (units: log (µM)) of the mother logMDA.father: Levels of lipid oxidative damage in plasma (malondialdehyde, MDA), log transformed (units: log (µM)) of the father mother.triglicerides: Plasma triglyceride levels of the mother, units: mg/dL father.triglicerides: Plasma triglyceride levels of the father, units: mg/dL 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: MDA: plasma lipid oxidative damage (malondialdehyde acid)