Uncovering a miltiradiene biosynthetic gene cluster in the Lamiaceae reveals a dynamic evolutionary trajectory. Abigail E. Bryson & Emily R. Lanier, Kin H. Lau, Davis Mathieu, Alan E. Yocca, Garret P. Miller, Patrick P. Edger, C. Robin Buell, Björn Hamberger. --- Genome Files: Assembly and annotation files for the genomes of Leonotis leonurus, Plectranthus barbatus, and Prunella vulgaris. Zipped files include: predicted cds, cdna, and amino acid sequences in fasta format, genome annotation file, genome assembly and fasta index for each species. Leonotis-leonurus.tar.gz Plectranthus-barbatus.tar.gz Prunella-vulgaris.tar.gz --- Collinearity Files: Generated from MCScanX analysis. Callicarpa americana as compared to Tectona grandis, Pogostemon cablin, Rosmarinus officinalis, Scutellaria baicalensis, Hyssopus officinalis, Salvia miltiorrhiza, Plectranthus barbatus, Leonotis leonurus, Prunella vulgaris,and Erythranthe lutea, respectively. Cam_Tgr.collinearity Cam_Pca.collinearity Cam_Rof.collinearity Cam_Sba.collinearity Cam_Hof.collinearity Cam_Smi.collinearity Cam_Pba.collinearity Cam_Lle.collinearity Cam_Pvu.collinearity Cam_Elu.collinearity --- Excel/CSV File: All amino acid sequences and genomic location of the genes and pseudogenes in all species examined. Also includes original naming conventions from respective assemblies. Color coded if opened with excel. All_BGC_Sequences.xlsx --- Newick Files: Alignment generated from ClustalOmega and 1000 bootstrap support from RAxML. RAxML_bipartitionsBranchLabels.TPS-c_Class2_NoTP_Tree RAxML_bipartitionsBranchLabels.TPS-e_Class1_NoTP_Tree RAxML_bipartitionsBranchLabels.CYP71Ds_Tree RAxML_bipartitionsBranchLabels.CYP76AHs_Tree --- Fasta Files: Sequence data used to make above phylogenies. Note: Transit Peptides have been removed. TPS-e_Class1_NoTP.txt TPS-c_Class2_NoTP.txt CYP76AHs.txt CYP71Ds.txt --- GCMS Files: Date of file name indicates date run on instrument. Retention times may vary between different dates. All include DXS + GGPPS even if not explicitly stated. Used in Fig. 7 and Supplementary figs. 3 & 5 (hexane extracts) A_20220321_hex_EL01.CDF | DXS + GGPPS control A_20220321_hex_EL03.CDF | CamTPS1 A_20220321_hex_EL04.CDF | CamTPS1 + NmTPS2 A_20220321_hex_EL05.CDF | CamTPS1 + CamTPS12 A_20220321_hex_EL06.CDF | CamTPS1 + CamTPS10 A_20220321_hex_EL07.CDF | CamTPS6 A_20220321_hex_EL08.CDF | CamTPS6 + PbTPS3 A_20220321_hex_EL09.CDF | CamTPS6 + CamTPS9 A_20220321_hex_EL10.CDF | CamTPS6 + CamTPS10 A_20220321_hex_EL11.CDF | CamTPS6 + CamTPS12 A_20220321_hex_EL13.CDF | CamTPS7 A_20220321_hex_EL14.CDF | CamTPS7 + CamTPS10 A_20220321_hex_EL15.CDF | CamTPS7 + CamTPS9 Used in Fig. 8a (ethyl acetate extracts) A_211015_004.CDF | DXS + GGPPS control A_211015_008.CDF | CamTPS6 + CamTPS9 A_211015_017.CDF | CamTPS6 + CamTPS9 + CamCYP76AH67 A_211015_020.CDF | CamTPS6 + CamTPS9 + CamCYP76AH67 + CamCYP76AH68 A_211015_024.CDF | CamTPS6 + CamTPS9 + CamCYP76AH68 Used in Fig. 8b and Supplementary fig. 7 A_20220128_002.CDF | DXS + GGPPS control A_20220128_005.CDF | CamTPS6 A_20220128_006.CDF | CamTPS6 + SsSS A_20220128_010.CDF | A_20220128_015.CDF | CamTPS6 + CamCYP71D716 A_20220128_016.CDF | CamTPS6 + CamCYP71D717 A_20220128_017.CDF | CamTPS6 + CamCYP71D716 + CamCYP71D717 A_20220128_018.CDF | CamTPS6 + SsSS + CamCYP71D716 LCMS file: Used in Supplementary fig. 9. Methanolic root extract of Callicarpa americana was analyzed using a Waters XEVO-G2XS QTOF with column Acquity BEH C18, 100x2.1mm, 1.7uM, s/n 03183814315196. LC method: Solvent A: 10 mm Ammonium formate (pH 2.8) in water, Solvent B: acetonitrile; 0 min: 99% solvent A, 1% solvent B; 16 min isocratic gradient to 2% solvent A, 98% solvent B; 16-18 min: flush with 99% solvent A. Column temperature of 40 °C. MS/MS was ESI in (+) mode, scan range 50-1500 Da. XS2_20211201_003.raw.tar.gz