GENERAL INFORMATION 1. Title of Dataset: Data from: Insecticidal activity of Beauveria bassiana against Monochamus alternatus adults 2. Author Information Corresponding Investigator Name: Prof. Jae Su Kim Institution: Jeonbuk national university, Jeonju, Korea Email: jskim10@jbnu.ac.kr Co-investigator 1 Name: Dr Jong-Cheol Kim Institution: Jeonbuk national university, Jeonju, Korea Email: jc.kim124@gmail.com Co-investigator 2 Name: Dr Mi Rong Lee Institution: Jeonbuk national university, Jeonju, Korea Co-investigator 3 Name: Jeong Seon Yu Institution: Jeonbuk national university, Jeonju, Korea Co-investigator 4 Name: So Fun Park Institution: Jeonbuk national university, Jeonju, Korea Co-investigator 5 Name: Panjung Ha Institution: Crop Protection R&D Center, Farm Hannong (LG Affiliated Co.), Nonsan, Korea 3. Date of data collection: 2017-2020 4. Geographic location of data collection: Jeonju, Nonsan, and Ulsan in Korea 5. Funding sources that supported the collection of the data: National Research Foundation of Korea DATA & FILE OVERVIEW 1. File List: File 1 Name: 1.Mortality of M. alternatus adullts in small pine trees pre-treated with B. bassiana ERL836 in pot conditions.xlsx File 2 Name: 2.Insecticidal activity of B. bassiana ERL836 against M. alternatus adults using fungal pre-treatment of pine logs artificially infested with larvae.xlsx File 3 Name: 3.Insecticidal activity of B. bassiana ERL836 against M. alternatus adults using formulated fungal powder pre-treatment of pine logs artificially infested with larvae in greenhouse conditions.xlsx File 4 Name: 5.Control efficacy of B. bassiana ERL836 against M. alternatus by fungal powder formulation pre-treatment of pine logs artificially infested with larvae in semi-field conditions.xlsx File 5 Name: 6.Control efficacy of B. bassiana ERL836 against M. alternatus by fungal powder formulation pre-treatment of naturally larvae-infested pine logs in forest field conditions.xlsx File 6 Name: 7ace.Transmission of B. bassiana ERL836 to non-target forest insects.xlsx File 7 Name: 7bdf.Transmission of B. bassiana ERL836 to non-target forest insects.xlsx DATA-SPECIFIC INFORMATION FOR: 1.Mortality of M. alternatus adullts in small pine trees pre-treated with B. bassiana ERL836 in pot conditions.xlsx 1. Number of variables: 4 2. Number of cases/rows: 234 3. Variable List: Treatment: NC, Bb-6, Bb-7 Day: Day after treatment; 0-25 Rep: Replicate; 3 Mortality: Mortality rate of Monochamus alternatus adults 4. Missing data codes: None 5. Abbreviations used: NC; Non-treated control, Bb-6; Beauveria bassiana (1.0*10^6 conidia/ml) treatment, Bb-7; Beauveria bassiana (1.0*10^7 conidia/ml) treatment 6. Other relevant information: The insecticidal activity of B. bassiana ERL836 against M. alternatus adults was evaluated by pre-treating pine trees (Pinus densiflora) with a fungal conidia suspension (1.0 ×106 or 1.0 ×107 conidia/ml) in pot conditions and then adding M. alternatus adults to the trees. DATA-SPECIFIC INFORMATION FOR: 2.Insecticidal activity of B. bassiana ERL836 against M. alternatus adults using fungal pre-treatment of pine logs artificially infested with larvae.xlsx 1. Number of variables: 5 2. Number of cases/rows: 186 3. Variable List: Treatment: NC, Bb Day: Day after treatment; 0-30 Rep: Replicate; 3 Emergence rate: emergence rate of Monochamus alternatus adults after treatment Mortality rate: mortality rate of Monochamus alternatus adults after escape from pine log 4. Missing data codes: None 5. Abbreviations used: NC; Non-treated control, Bb; Beauveria bassiana ERL836 treatment 6. Other relevant information: The conidia suspension of B. bassiana ERL836 was sprayed to pine logs with M. alternatus. Emerging adults and dead and alive adults were counted daily for 30 days after the emergence of the first adults. DATA-SPECIFIC INFORMATION FOR: 3.Insecticidal activity of B. bassiana ERL836 against M. alternatus adults using formulated fungal powder pre-treatment of pine logs artificially infested with larvae in greenhouse conditions.xlsx 1. Number of variables: 5 2. Number of cases/rows: 465 3. Variable List: Treatment: NC, Bb Day: Day after treatment; 0-30 Rep: Replicate; 3 Emergence rate: emergence rate of Monochamus alternatus adults after treatment Mortality rate: mortality rate of Monochamus alternatus adults after escape from pine log 4. Missing data codes: None 5. Abbreviations used: NC; Non-treated control, BbP1, Beauveria bassiana ERL836 (1 g/box) treatment; BbP10; Beauveria bassiana ERL836 (10 g/box) treatment, BbS100; Beauveria bassiana ERL836 (100× spray) treatment, BbS10; Beauveria bassiana ERL836 (10× spray) treatment 6. Other relevant information: The B. bassiana ERL836 powder formulation was diluted in water for spray or directly powered to pine logs. For the powder-based suspension spray, 1 and 10 g of fungal powder formulation was diluted with 100 ml water to prepare 1% and 10% suspensions, respectively. Emerging adults and dead and alive adults were counted daily for 30 days after the emergence of the first adults. DATA-SPECIFIC INFORMATION FOR: 5.Control efficacy of B. bassiana ERL836 against M. alternatus by fungal powder formulation pre-treatment of pine logs artificially infested with larvae in semi-field conditions 1. Number of variables: 7 2. Number of cases/rows: 36 3. Variable List: Treatment: NC, Bb Day: investigation day; 5 Jun, 12 Jun, 19 Jun, 26 Jun, 03 Jul, 10 Jul Rep: Replicate; 3 Emerged adults: number of emerged Monochamus alternatus adults after treatment Mortality rate: number of dead Monochamus alternatus adults on investigation day MaM: mortality after moisturizing for 14 days Total mortality: Mortality rate + MaM 4. Missing data codes: None 5. Abbreviations used: NC; Non-treated control, Bb; Beauveria bassiana ERL836 powder 100 g/box treatment, MaM; mortality after moisturizing for 14 days 6. Other relevant information: The semi-field test was performed from spring to fall (April to July 2020) at FarmHannong’s test field (36°07'58.1"N 127°07'45.4"E, Nosan, Korea). Twelve pine logs (ca. 1 m) with five final-stage larva (about 60 M. Alternatus larvae) were piled, and 100 g of 2.5% formulated B. Bassiana ERL836 fungal powder formulation were treated on the piled pine logs. The fungal-treated pine logs were covered with a covering film and sealed. Each treatment was tested in triplicate. The number of emerging adults and mortality of M. alternatus adults were monitored weekly. DATA-SPECIFIC INFORMATION FOR: 6.Control efficacy of B. bassiana ERL836 against M. alternatus by fungal powder formulation pre-treatment of naturally larvae-infested pine logs in forest field conditions.xlsx 1. Number of variables: 7 2. Number of cases/rows: 72 3. Variable List: Treatment: NC, Bb100, Bb200 Day: investigation day; 17 Jun, 24 Jun, 01 Jul, 08 Jul, 15 Jul, 22 Jul, 29 Jul, 05 Aug Rep: Replicate; 3 Emerged adults: number of emerged Monochamus alternatus adults after treatment Mortality rate: number of dead Monochamus alternatus adults on investigation day MaM: mortality after moisturizing for 14 days Total mortality: Mortality rate + MaM 4. Missing data codes: None 5. Abbreviations used: NC; Non-treated control, Bb100; Beauveria bassiana ERL836 powder 100 g treatment, Bb200; Beauveria bassiana ERL836 powder 200 g treatment, MaM; mortality after moisturizing for 14 days 6. Other relevant information: The field test was performed from fall to summer (November 2019 to July 2020) in a forest area where pine wilt nematode occurred (35°29'52.0"N 129°17'35.8"E, Ulsan, Korea). Near that forest area, pine logs (ca. 1 m) naturally infested with M. alternatus larvae were collected with the assistance of a local forest manager during the winter (November to December 2019). The 2.5% formulation of B. bassiana ERL836 fungal powder was treated to about 50 pine logs presumed to contain about 200 M. alternatus larvae, piled to about 1 m3, covered with a covering film (tarpaulin), and sealed. To evaluate the insecticidal activity according to the amount of fungal powder formulation, 100 g and 200 g of the B. bassiana ERL836 fungal powders were treated, and the non-treated control was not treated with any fungal powder. The number of emerging adults and the mortality of M. alternatus adults were monitored weekly. DATA-SPECIFIC INFORMATION FOR: 7ace.Transmission of B. bassiana ERL836 to non-target forest insects.xlsx 1. Number of variables: 5 2. Number of cases/rows: 180 3. Variable List: Treatment: NC, Bb Insect: Ad, Bm, Lm Day: Day after treatment; 0-14 Rep: Replicate; non-treated control 1, Beauveria bassiana ERL836 treatment 3 Mortality: Mortality of the three insects after treatment 4. Missing data codes: None 5. Abbreviations used: NC; Non-treated control, Bb; Beauveria bassiana ERL836 treatment, Ad; Allomyrina dichotoma adult, Bm; Bombyx mori larva, Lm; Lucanus maculifemoratus adult 6. Other relevant information: The direct insecticidal activity of B. bassiana ERL836 against the three non-target forest insects was investigated in lab conditions. The three non-target insects were A. dichotoma adult, L. maculifemoratus adult, and B. mori larvae. DATA-SPECIFIC INFORMATION FOR: 7bdf.Transmission of B. bassiana ERL836 to non-target forest insects.xlsx 1. Number of variables: 6 6 2. Number of cases/rows: 360 3. Variable List: Treatment: NC, Bb NTI: non-target insects; Ad, Bm, Lm Day: Day after treatment; 0-14 Rep: Replicate; non-treated control 2, Beauveria bassiana ERL836 treatment 6 MoTMI: Mortality of Transmitter insect MoNTI: Mortality of Non target insect 4. Missing data codes: None 5. Abbreviations used: NC; Non-treated control, Bb; Beauveria bassiana ERL836 treatment, NTI; non-target insect, Ad; Allomyrina dichotoma adult, Bm; Bombyx mori larva, Lm; Lucanus maculifemoratus adult, MoTMI; Mortality of Transmitter insect (Monochamus alternatus adult), MoNTI; Mortality of Non target insect 6. Other relevant information: The transmission potential of B. bassiana ERL836 to the non-target forest insects was confirmed using fungus-infected M. alternatus adults.