GENERAL INFORMATION Title of Dataset: Dataset for ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription–free exponential amplification reaction, RTF-EXPAR Principal Investigator Information Name: Jim Tucker ORCID:https://orcid.org/0000-0001-7645-0815 Institution: University of Birmingham Address: University of Birmingham, Edgbaston, Birmingham, UK, B15 2TT Email: j.tucker@bham.ac.uk Principal Investigator Information Name: Tim Dafforn ORCID: https://orcid.org/0000-0003-2257-6679 Institution: University of Birmingham Address: University of Birmingham, Edgbaston, Birmingham, UK B15 2TT Email: t.r.dafforn@bham.ac.uk Date of data collection: August2021 to May2022 Geographic location of data collection: Birmingham, UK Information about funding sources that supported the collection of the data: BBSRC SHARING/ACCESS INFORMATION Licenses/restrictions placed on the data: none Links to publications that cite or use the data: https://www.pnas.org/doi/full/10.1073/pnas.2100347118 DATA & FILE OVERVIEW File List: There are four C# files and thirty-six txt files. More information on these files is given in the data-specific information section. Config.cs Cycle.cs Program.cs Well.cs Figure 2.txt Figure 3 EXPAR 10 Sigma Time.txt Figure 3 EXPAR Normalised Data.txt Figure 3 EXPAR Raw Data.txt Figure 3 LAMP 10 Sigma Time.txt Figure 3 LAMP Raw Data.txt Figure 3 PCR 10 Sigma Time.txt Figure 3 PCR Normalised Data.txt Figure 3 PCR Raw Data.txt Figure 4 EXPAR 10 Sigma Time.txt Figure 4 EXPAR Normalised Data.txt Figure 4 EXPAR Raw Data.txt Figure 4 LAMP 10 Sigma Time.txt Figure 4 LAMP Raw Data.txt Figure 4 PCR 10 Sigma Time.txt Figure 4 PCR Raw Data.txt Figure 5 10 Sigma Time.txt Figure 5 Normalised Data.txt Figure 5 Raw Data.txt Figure S1 10 Sigma Time.txt Figure S1 Normalised Data.txt Figure S1 Raw Data.txt Figure S2 10 Sigma Time.txt Figure S2 Normalised Data.txt Figure S2 Raw Data.txt Figure S3 10 Sigma Time.txt Figure S3 Normalised Data.txt Figure S3 Raw Data.txt Figure S4 10 Sigma Time.txt Figure S4 Normalised Data.txt Figure S4 Raw Data.txt Figure S5 10 Sigma Time.txt Figure S5 Normalised Data.txt Figure S5 Raw Data.txt Figure S6 10 Sigma Time.txt Figure S6 Normalised Data.txt METHODOLOGICAL INFORMATION Description of methods used for collection/generation of data: All methods are described in the following publication: https://www.pnas.org/doi/full/10.1073/pnas.2100347118 Methods for processing the data: All methods are described in the following publication: https://www.pnas.org/doi/full/10.1073/pnas.2100347118 DATA-SPECIFIC INFORMATION: 1) all txt files All files refer to fluorescence amplification curves and bar chart data presented in the relevant numbered figures in the publication: https://www.pnas.org/doi/full/10.1073/pnas.2100347118. Fluorescence data was collected and exported as a plain text file for data processing (primary data files). Normalised data was determined using the normalise function in GraphPad Prism 9 (normalised data files). The fluorescence data is given in arbitrary units and any multiple columns with the same heading (e.g. three "positive control" columns) are due to three repeats. Some column headers in txt files with + and - signs refer to positive and negative controls respectively, as presented in bar charts in the PNAS paper. 2) all .cs files To generate run times for the fluorescence amplification data, a program in C# was developed. The program analyses the first 10 data points and calculates the mean value and SD as a base line. Following generation of these two values, each subsequent data point was analyzed to determine if its value minus the average value was greater than 10 SDs away from the mean. The cycle which met this criterion was converted into a time and used as the minimum amplification time, as presented in the publication https://www.pnas.org/doi/full/10.1073/pnas.2100347118