This readme file was generated on [2022-07-05] by [Mia Park] GENERAL INFORMATION Title of Datasets: Effects of Temperature and Wildflower Strips on Survival and Macronutrient Stores of the Alfalfa Leafcutting Bee (Hymenoptera: Megachilidae) Under Extended Cold Storage Journal: Environmental Entomology Manuscript ID: ENVENT-2022-0089 Author 1: Mia G. Park, North Dakota State University, Department of Biological Sciences, Fargo, ND, miagpark@gmail.com Author 2: Casey Delphia, Departments of Biological Sciences and Land Resources and Environmental Sciences, Montana State University, Bozeman, MT Author 3: Cassandra Prince, Edward T. Schafer Agricultural Research Center, Biosciences Research Laboratory,Fargo, ND Author 4: George Yocum, Edward T. Schafer Agricultural Research Center, Biosciences Research Laboratory,Fargo, ND Author 5: Joseph Rinehart, Edward T. Schafer Agricultural Research Center, Biosciences Research Laboratory,Fargo, ND Auhtor 6: Kevin M. O’Neill, Montana State University, Land Resources and Environmental Sciences, Bozeman, MT Author 7: Laura A. Burkle, Montana State University, Department of Ecology, Bozeman, MT Author 8: Julia Bowsher, North Dakota State University, Department of Biological Sciences, Fargo, ND Author 9: Kendra Greenlee, North Dakota State University, epartment of Biological Sciences, Fargo, ND Abstract: Megachile rotundata (F.) is an important pollinator of alfalfa in the United States. Enhancing landscapes with wildflowers is a primary strategy for conserving pollinators and may improve sustainability of M. rotundata. Changing cold storage temperatures from a traditionally static thermal regime (STR) to a fluctuating thermal regime (FTR) improves overwintering success and extends M. rotundata’s shelf life and pollination window. Whether floral resources enhance overwintering survival and/or interact with thermal regime are unknown. With these data sets we tested the combined effects of enhancing alfalfa fields with wildflowers and thermal regime on survival and macronutrient stores under extended cold storage (i.e., beyond one season). Megachile rotundata adults were released in alfalfa plots with and without wildflower strips summer 2017. Completed nests were harvested in September 2017 and stored in STR. After a year, cells were randomly assigned to remain in STR for 6 months or in FTR for a year of extended cold storage; emergence rates were observed monthly. Macronutrient -- total simple sugar, glycogen, trehalose and total lipid -- levels of emerged females were assessed. Research domain: 1.06 Biological sciences Dates of data collection: 2018-08-01 to 2020-07-15 Geographic location of data collection: Field: Bozeman, MT; Lab: Fargo, ND, USA DATA & FILE OVERVIEW File List: sugars.csv, trehalose.csv, lipids.csv, glycogen.csv, emergence.csv Relationship between files, if important: sugars.csv, trehalose.csv, lipids.csv and glycogen.csv are parallel data sets that follow macronutrients levels of newly emerged, female Megachile rotundata at 12, 14 and 16 months in cold storage. Twelve months is baseline before temperature treatments applied, 14 months marks time point before survival diverges between STR and FTR, and 16 months marks time point after which survival diverges. emergence.csv contains survival data from initiation of temperature treatments at 12 months to end of FTR treatment at 24 months in cold storage. METHODOLOGICAL INFORMATION Description of methods used for collection/generation of data: see manuscript for methodological details. DATA-SPECIFIC INFORMATION FOR: [sugars.csv] Number of variables: 12 Number of cases/rows: 221 Variable List: 1. new.id: unique id given to each bee specimen 2. month: month of emergence; sep = September 2018, nov = November 2018, jan = January 2019 3. temp: temperature regime treatment, either static (STR) or fluctuating (FTR) temperature regime 4. week: week of nest completion after initial release of bees in field; 2 is early and 6 is late in season 5. flr.trt: wildflower treatment; alf = alfalfa only or alf+ = alfalfa with wildflower strips 6. plot: id for field research plot (1-6) 7. DW.ug: freeze-dried weight (ug) 8. ug.total: total sugars (ug) 9. plate: unique id for each microplate used during assays 10. date: date of assay 11. prop.sug: proportion sugars (ug total sugars/ug freeze-dried weight) 12. mo: number of months in cold storage DATA-SPECIFIC INFORMATION FOR: [glycogen.csv] Number of variables: 12 Number of cases/rows: 210 Variable List: 1. new.id: unique id given to each bee specimen 2. month: month of emergence; sep = September 2018, nov = November 2018, jan = January 2019 3. temp: temperature regime treatment, either static (STR) or fluctuating (FTR) temperature regime 4. week: week of nest completion after initial release of bees in field; 2 is early and 6 is late in season 5. flr.trt: wildflower treatment; alf = alfalfa only or alf+ = alfalfa with wildflower strips 6. plot: id for field research plot (1-6) 7. DW.ug: freeze-dried weight (ug) 8. date: date of assay 9. plate: unique id for each microplate used during assays 10. ug.total: total glycogen (ug) 11. prop.glycogen: proportion glycogen (ug glycogen/ug freeze-dried weight) 12. mo: number of months in cold storage DATA-SPECIFIC INFORMATION FOR: [trehalose.csv] Number of variables: 12 Number of cases/rows: 220 Variable List: 1. new.id: unique id given to each bee specimen 2. month: month of emergence; sep = September 2018, nov = November 2018, jan = January 2019 3. temp: temperature regime treatment, either static (STR) or fluctuating (FTR) temperature regime 4. week: week of nest completion after initial release of bees in field; 2 is early and 6 is late in season 5. flr.trt: wildflower treatment; alf = alfalfa only or alf+ = alfalfa with wildflower strips 6. plot: id for field research plot (1-6) 7. DW.ug: freeze-dried weight (ug) 8. date: date of assay 9. plate: unique id for each microplate used during assays 10. ug.total: total trehalose (ug) 11. prop.trehalose: proportion glycogen (ug trehalose/ug freeze-dried weight) 12. mo: number of months in cold storage DATA-SPECIFIC INFORMATION FOR: [lipids.csv] Number of variables: 12 Number of cases/rows: 222 Variable List: 1. new.id: unique id given to each bee specimen 2. month: month of emergence; sep = September 2018, nov = November 2018, jan = January 2019 3. temp: temperature treatment, either static (STR) or fluctuating (FTR) thermal regime 4. week: week of nest completion after initial release of bees in field; 2 is early and 6 is late in season 5. flr.trt: wildflower treatment; alf = alfalfa only or alf+ = alfalfa with wildflower strips 6. plot: id for field research plot (1-6) 7. DW.ug: freeze-dried weight (ug) 8. date: date of assay 9. plate: unique id for each microplate used during assays 10. ug.total: total lipids (ug) 11. lip.prop: proportion total lipids (ug lipids/ug freeze-dried weight) 12. mo: number of months in cold storage DATA-SPECIFIC INFORMATION FOR: [emergence.csv] Number of variables: 11 Number of cases/rows: 9515 Variable List: 1. mo: number of months in cold storage n: unique id given to each bee specimen 2. month: month of emergence, sep 2018 - aug 2019 3. temp: temperature treatment, either static (STR) or fluctuating (FTR) thermal regime 4. plot: id for field research plot (1-6) 5. flora: wildflower treatment; 'alfalfa' = alfalfa only or 'alfalfa+' = alfalfa with wildflower strips 6. week: week of nest completion after initial release of bees in field 7. time: early v. late nesting within flight season; categorized early weeks=2-4, late weeks = 5-7 8. id: unique specimen id within each research plot for each month x temperature treatment combination 9. sex: M = male, F= female, NA = no data 10. em: emergence success, 1 = emerged, 0 = failed to emerge, PARA = parasitized, PB = pollen ball, NA = no data 11. unem: stage of development for those specimens that did not emerge; EM = emerged, LARV = unemerged larva, PRE = unemerged prepupa, PUPA: unemerged pupa, DAB : unemerged adult