Nutritional stress exacerbates impact of a novel insecticide on solitary bees’ behaviour, reproduction and survival Anina C. Knauer, Cedric Alaux, Matthew J. Allan, Robin R. Dean, Virginie Dievart, Gaétan Glauser, Tomasz Kiljanek, Denis Michez, Janine M. Schwarz, Giovanni Tamburini, Dimitry Wintermantel, Alexandra Maria Klein and Matthias Albrecht Data description File: Data_Knauer etal_PRSB_Osmia insecticide nutrition.xlsx Description: Data from the semi-field experiment used to analyse effects of Flupyradifurone exposure and nutritional stress on the following fitness proxies in Osmia bicornis: survival, offspring production, activity, flight duration, nest_recognition, foraging efficiency and offspring performance. Variables in data: cage: cage identity nutrition_treatment: plant identity in cage [BUC: Fagopyrum esculentum, PHA: Phacelia tanacetifolia, SEN: Sinapis arvensis] pesticide treatment: pesticide treatment applied to cage [P: application of Sivanto prime (active ingredient flupyradifurone), C: water control] ass_day: assessment day after application of Sivanto prime(active ingredient flupyradifurone) time: day time bee_id: identity of single bees, identified by tag on thorax nest_id: identity of single nest cavity survival: survival of females during assessment day [1: survived, 0: died] cells_built: number of cells built by single females within whole assessment day females_act: foraging activity of females during assessment day [1: female active, 0: female not active] duration: flight duration [hh:mm:ss] holes_probed: the number of nest cavities a female entered before finding its own observer: initials of the observer flowers_per_minute: the average number of flowers the observed bee visited per minute hatching: hatching of offspring bee in spring [1: hatched, 0: not hatched] sex: sex of offspring bee [1: female, 0: male] body_size: weight of offspring bee [mg] nutrition_quality: pca on foraging preference, flupyradifurone clearance and vitellogenin gene expression measured in bees foraging on the different food plants nutrition_stress: nutrition quality reversed