This README_DuellEtAl_EABVColdPlasticity.txt file was generated on 2022-01-18 by CJK MacQuarrie GENERAL INFORMATION 1. Title of Dataset: Data from: Plasticity drives extreme cold tolerance of emerald ash borer (Agrilus planipennis) during a polar vortex 2. Author Information A. Principal Investigator Contact Information Name: Meghan Duell Institution: Department of Biology, Western University Address: Department of Biology, University of Western Ontario, London, Ontario, Canada, N6A 5B7 Email: mduell@uwo.ca B. Associate or Co-investigator Contact Information Name: Brent Sinclair Institution: Department of Biology, Western University Address: Department of Biology, University of Western Ontario, London, Ontario, Canada, N6A 5B7 Email: bsincla7@uwo.ca C. Alternate Contact Information Name: Chris MacQuarrie Institution: Natural Resources Canada Canadian Forest Service Address: 1219 Queen St E., Sault Ste. Marie, Ontario, Canada, P6A 2E5 Email: christian.macquarrie@nrcan-rncan.gc.ca 3. Date of data collection 2018-11-21 to 2021-03-31 (approx) 4. Geographic location of data collection: Winnipeg, Manitoba, Canada, Barrie, Ontario, Canada and London, Ontario, Canada 5. Information about funding sources that supported the collection of the data: This work was supported by Natural Resources Canada Great Lakes Forestry Centre/Western Science joint funding grant and a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant and Discovery Accelerator Supplement. SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: none 2. Links to publications that cite or use the data: Duell et al. 2022. Plasticity drives extreme cold tolerance of emerald ash borer (Agrilus planipennis) during a polar vortex, Current Research in Insect Science. 3. Links to other publicly accessible locations of the data: none 4. Links/relationships to ancillary data sets: none 5. Was data derived from another source? no 6. Recommended citation for this dataset: Meghan E. Duell, Meghan T. Gray, Amanda D. Roe, Chris J. K. MacQuarrie, Brent J. Sinclair. 2022. Data from: Plasticity drives extreme cold tolerance of emerald ash borer (Agrilus planipennis) during a polar vortex. DATA & FILE OVERVIEW 1. File List: Winnipeg EAB data.xlsx (contains all data in 9 associated sheets) 2. Relationship between files, if important: N/A 3. Additional related data collected that was not included in the current data package: N/A 4. Are there multiple versions of the dataset? no METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: See methods description in associated manuscript. 2. Methods for processing the data: See methods description in associated manuscript. 3. Instrument- or software-specific information needed to interpret the data: See methods description in associated manuscript. 4. Standards and calibration information, if appropriate: N/A 5. Environmental/experimental conditions: N/A 6. Describe any quality-assurance procedures performed on the data: N/A 7. People involved with sample collection, processing, analysis and/or submission: Corresponds to author list in associated manuscript. //Following is data-specific information is for each sheet in the .xlsx file// DATA-SPECIFIC INFORMATION FOR: metadata 1. Number of variables: N/A (gives metadata for all other sheets) 2. Number of cases/rows: N/A 3. Variable List: N/A 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: 2020-21 London outdoor temps 1. Number of variables: 3 2. Number of cases/rows: 769 3. Variable List: Date: collection date in m/dd/yyyy format Time: collection time in hh:mm format, Eastern Standard Time (UTC-5) Air temp: observed air temperature from an Onset Hobo UI2 ldatalogger with a shielded thermistor at 1.2 m near the bolts stored outside in London, ON, in Celsius 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used:N/A DATA-SPECIFIC INFORMATION FOR: Winnipeg biome temps 1. Number of variables: 3 2. Number of cases/rows: 820 3. Variable List: Date: collection date in m/dd/yyyy format Time: collection time in hh:mm format, Eastern Standard Time (UTC-5) Temperature: Temperature program for Winnipeg simulated winter conditions, in Celsius 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: SCPs 2018-2019 (Supercooling points of larvae and prepupae collected from and overwintered in Ontario and Winnipeg, MB.) 1. Number of variables: 3 2. Number of cases/rows: 84 3. Variable List: Source: Geographic location where insects were obtained (see methods in associated manuscript) Life stage stage of emerald ash borer that was measured SCP: supercooling point of insect, in Celsius 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: SCPs 2020-2021 (Supercooling points of larvae and prepupae collected in Winnipeg, MB and overwintered either outdoors in London, ON, or in a simulated Winnipeg winter in the laboratory.) 1. Number of variables: 3 2. Number of cases/rows: 46 3. Variable List: Source: Geographic location where insects were obtained (see methods in associated manuscript) Life stage stage of emerald ash borer that was measured SCP: supercooling point of insect, in Celsius 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: low temp survival 2019 (Survival of larvae and prepupae collected from and overwintered in Ontario and Winnipeg, MB after a 1 h exposure to different temperatures.) 1. Number of variables: 6 2. Number of cases/rows: 241 3. Variable List: temp: treatment temperature, in Celsius Source: Geographic location where insects were obtained (see methods in associated manuscript) Life Stage: Life stage stage of emerald ash borer that was measured individual: number of individual within replicate (Individuals were not exposed more than once) alive: 1= alive 24 h after rewarming; 0=dead 24 h after rewarming. Froze?: y indicates that a supercooling point was recorded from an individual. 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: survival counts (A summary of mortality in all animals extracted from bolts in this study.) 1. Number of variables: 9 2. Number of cases/rows: 37 3. Variable List: Year: Year of study Bolt: Individually numbered within year and source Source: Geographic location where insects were obtained (see methods in associated manuscript) EAB Prepupae living: total number of live animals in a bolt. Includes those accidentally killed or damaged during extraction and therefore not used in experiments. EAB Prepupae dead: total number of dead prepupae in a bolt. EAB larvae living: total number of live larvae in a bolt, including those killed or damaged during extraction and not used in experiments. EAB larvae dead: total number of dead larvae in a bolt. Parasitoids present?: Presence or absence of parasitoids associated with dead larvae or prepupae in a bolt. No. parasitoids: total number of partasitoids in a bolt. 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: Osmolality & Glycerol 2018-19 (Hemolymph composition measurements for larvae and prepupae either collected from Barrie, ON and overwintered in London or collected from and overwintered in Winnipeg, MB. Collections were in 2018, measurements were made in February 2019.) 1. Number of variables: 5 2. Number of cases/rows: 41 3. Variable List: Life Stage: Life stage stage of emerald ash borer that was measured Source: Geographic location where insects were obtained (see methods in associated manuscript) Individual Number: each individual is numbered within a source/life stage. Glycerol and osmolality measurements were made on hemolymph from the same individual. Glycerol: Hemolymph glycerol concentration in M/l. Osmolality: Hemolymph osmolality in Osm/kg. 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: Osmolality & Glycerol 2020-21 (Hemolymph composition measurements for larvae and prepupae collected from Winnipeg, MB in autumn 2020 and either overwintered outdoors in London, ON or overwintered in the laboratory under simulated Winnipeg winter conditions. Measurements were made in February 2021.) 1. Number of variables: 5 2. Number of cases/rows: 15 3. Variable List: Life Stage: Life stage stage of emerald ash borer that was measured Source: Geographic location where insects were obtained (see methods in associated manuscript) Individual Number: each individual is numbered within a source/life stage. Glycerol and osmolality measurements were made on hemolymph from the same individual. Glycerol: Hemolymph glycerol concentration in M/l. Osmolality: Hemolymph osmolality in Osm/kg. 4. Missing data codes: N/A 5. Specialized formats or other abbreviations used: N/A DATA-SPECIFIC INFORMATION FOR: Mass and water content 2020-21 (Hemolymph composition measurements for larvae and prepupae collected from Winnipeg, MB in autumn 2020 and either overwintered outdoors in London, ON or overwintered in the laboratory under simulated Winnipeg winter conditions. Measurements were made in February 2021.) 1. Number of variables: 7 2. Number of cases/rows: 3. Variable List: 63 date extracted Life Stage: Life stage stage of emerald ash borer that was measured Source: Geographic location where insects were obtained (see methods in associated manuscript) fresh mass: mass of insect, in milligrams dry mass: mass of insect, in milligrams, after drying at 60 °C for 7 d water mass: water mass in milligrams notes: notes on sample. 4. Missing data codes: blank cell indicates no data for that variable 5. Specialized formats or other abbreviations used: N/A