# Data included here: the final transcriptome incl. functional annotation, GO annotation table, final gene counts matrix, and phenotypic data. ## Final transcriptome incl. functional annotation File: ```stringtie_merged_v2_sub_cor_annotated.gff``` Metadata: The transcriptome .gff file as produced by StringTie assembly (see scripts on GitHub for details), with functional annotation added in the last columns following convention. ## GO annotation table File: ```TransfExp_WM_gene2GO_v2.txt``` Metadata: Each row starts with the GeneID (which links back to the final transcriptome produced by StringTie, included in this deposition) followed by the GO terms annotated to this gene. ## Final gene counts matrix File: ```TransfExp2019_gene_count_matrix_v3cor.csv``` Metadata: This .csv file contains the gene counts for each sequenced RNA sample, determined by transcript quantification with StringTie at the gene level (see scripts on GitHub for details). See for experimental design details the Molecular Ecology publication. Columns - geneID # this geneID links back to the final transcriptome produced by StringTie, included in this deposition - next 84 columns = 1 column for each sample # these column names link back to the sample names in the raw RNAseq reads (see ENA deposition); column names contain Sampling information (e.g. C_3_102 = sample taken at 3h after transfer from the control constant 10oC to the cold constant 5oC, from clutch MotherID 102); Treatments: C=cold 5oC, W=warm 15oC, N=normal constant 10oC; Timepoints: 0h (i.e. BEF=before at constant 10oC), 3h, 24h ## Phenotypic data File: ```RNA_available-clutches2019.csv``` Metadata: This .csv file contains the observed median development stage for each clutch of winter moth eggs used in the experiment, determined with fluorescence microscopy (see Van Dis et al. 2021, J Exp Biol 224(17) for details). Medians and quantiles were taken from individual observations of embryos (data not included). Columns - Year #Year in which samples were taken - box + position #SampleID - Tube #MotherID - Treat_week #Development week in which sample was taken (in weeks after start of the experiment on Dec 14 2018) - NovemberDate #Catch date of mother in November days - Area #Area where mother was caught (all from forest in Doorwerth, the Netherlands) - Site + Tree #TreeID mother was caught on - dst50_before.0% + dst50_before.25% + dst50_before.50% + dst50_before.75% + dst50_before.100% #quantiles of development stages observed for clutch (stages 1-20), 50% quantile gives the median - Used? # Sample imaged with fluorescence microscopy Yes or No - Notes