README 17-Aug-2022 Rachel Turba (email for contact: rturba@outlook.com) Details of dataset related to manuscript published in Molecular Ecology: "Genetic structure and historic demography of endangered unarmored threespine stickleback at southern latitudes signals a need for new management directives" 1. 12gen_fastq.zip: fastq files of 12 low coverage genomes in the paper. Samples were pooled in two separate lanes. Therefore, names follow the structure: ..1: Collection ID Population Name CCS-08-32.1 San Antonio Creek Lagoon CCS-08-32.2 San Antonio Creek Lagoon RNF8220 San Antonio Creek VAFB RNF8218 San Antonio Creek VAFB CCS-03-87.1 Santa Clara Lagoon CCS-03-87.2 Santa Clara Lagoon RNF8116 Santa Clara River, Soledad Canyon RNF8117 Santa Clara River, Soledad Canyon RNF2867.1 Santa Ana River, Sugarloaf RNF2867.2 Santa Ana River, Sugarloaf DKJ-06NOV29.2 Bocana El Rosario DKJ-06NOV29.3 Bocana El Rosario 2. msat_data.gen: genepop file of microsatellite data for the individuals and populations listed on Table 1 in the manuscript.