This dataset describes the diet of a tropical anuran assemblage. We report the diet composition of 35 species collected at Yasuní National Park, based on the examination of the gastrointestinal contents of frog specimens available in the Museo de Zoología (QCAZ) of Pontificia Universidad Católica del Ecuador. Table 1. Anuran-prey interaction matrix. Number of each prey category found in 35 anuran species. Table 2. Prey volume matrix. Volume in mm3 of each prey category found in 35 anuran species, estimated from intact prey items. Table 3. Intact prey items. Numbers of intact prey items found in each anuran species that were used to estimate prey volume. Table 4. Anuran specimens. Available information of 396 specimens examined: museum number, family and species name, date and place of collection of anuran specimens examined. Anuran species names are abbreviated as follow (Ama = Amazophrynella, All = Allobates, Ame = Ameerega, Hyl = Hyloxalus, Aga = Agalychnis, Boa = Boana, Den = Dendropsophus, Nyc = Nyctimantis, Ost = Osteocephalus, Phy = Phyllomedusa, Sci = Scinax, Tra = Trachycephalus, Eda = Edalorhina, Eng = Engystomops, Pri = Pristimantis).