GENERAL INFORMATION 1. Title of Dataset: Genomic editing in a CRISPR/Cas9-based mouse model of PRPF31 retinitis pigmentosa 2. Author Information A. Principal Investigator Contact Information Name: Leah Byrne Institution: University of Pittsburgh Address: 3501 5th Ave. Pittsburgh, PA 15213 Email: lbyrne@pitt.edu 3. Date of data collection: 2022-03-01 4. Geographic location of data collection: Pittsburgh, PA 5. Information about funding sources that supported the collection of the data: Funding was provided by the NEI/NIH (UG3MH120094) to LB, and R01EY030991 to YC, Research to Prevent Blindness (LB; Career Development Award), Foundation Fighting Blindness, and the UPMC Immune Transplant and Therapy Center (LB), scholarship from China Scholarship Council, research fund from The Third Xiangya Hospital and Xiangya School of Medicine to ZX. RNA sequencing was performed at the University of Pittsburgh Genomics Research Core. We acknowledge support from NIH CORE Grant P30 EY08098 to the Department of Ophthalmology, from the Eye and Ear Foundation of Pittsburgh, and from an unrestricted grant from Research to Prevent Blindness, New York, NY. SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: None 2. Links to publications that cite or use the data: None 3. Links to other publicly accessible locations of the data: None 4. Links/relationships to ancillary data sets: None 5. Was data derived from another source? No 6. Recommended citation for this dataset: Please cite Dryad generated DOI DATA & FILE OVERVIEW 1. File List: Dataset 1 Subretinal injection-double vectors-WT mouse: 5wgh-5OS_S8_L001_R1_001.fastq.gz 5wgh-5OS_S8_L001_R2_001.fastq.gz 5wgh-6OS_S4_L001_R1_001.fastq.gz 5wgh-6OS_S4_L001_R2_001.fastq.gz 5wgh-7OS_S1_L001_R1_001.fastq.gz 5wgh-7OS_S1_L001_R2_001.fastq.gz Dataset 2 Intravitreal injection-single vector-Cas9 mouse: 5wCas9IVT-1_S7_L001_R1_001.fastq.gz 5wCas9IVT-1_S7_L001_R2_001.fastq.gz 5wCas9IVT-2_S1_L001_R1_001.fastq.gz 5wCas9IVT-2_S1_L001_R2_001.fastq.gz 5wCas9IVT-3_S2_L001_R1_001.fastq.gz 5wCas9IVT-3_S2_L001_R2_001.fastq.gz Dataset 3 Subretinal injection-gene augmentation-WT mouse: KO-PBS: 1OS_S5_L001_R1_001.fastq.gz 1OS_S5_L001_R2_001.fastq.gz 2OS_S3_L001_R1_001.fastq.gz 2OS_S3_L001_R2_001.fastq.gz 3OS_S6_L001_R1_001.fastq.gz 3OS_S6_L001_R2_001.fastq.gz KO-Rescue: 1OD_S2_L001_R1_001.fastq.gz 1OD_S2_L001_R2_001.fastq.gz 2OD_S1_L001_R1_001.fastq.gz 2OD_S1_L001_R2_001.fastq.gz 3OD_S4_L001_R1_001.fastq.gz 3OD_S4_L001_R2_001.fastq.gz Dataset 4 Human retinal explant-7m8gRNAa&b-filtered: 7ab-1_S3_R1.cutadapt.fastq.gz 7ab-1_S3_R2.cutadapt.fastq.gz 7ab-2_S4_R1.cutadapt.fastq.gz 7ab-2_S4_R2.cutadapt.fastq.gz 7ab-3_S2_R1.cutadapt.fastq.gz 7ab-3_S2_R2.cutadapt.fastq.gz 7ab-4_S1_R1.cutadapt.fastq.gz 7ab-4_S1_R2.cutadapt.fastq.gz Dataset 5 Subretinal injection-single vector-Cas9 mouse: 5wCas9SRi-1_S5_L001_R1_001.fastq.gz 5wCas9SRi-1_S5_L001_R2_001.fastq.gz 5wCas9SRi-2_S6_L001_R1_001.fastq.gz 5wCas9SRi-2_S6_L001_R2_001.fastq.gz 5wCas9SRi-3_S3_L001_R1_001.fastq.gz 5wCas9SRi-3_S3_L001_R2_001.fastq.gz METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: PCR fragments containing the targeted site of gRNA were amplified from gDNA using primers with partial Illumina adapter sequences. PCR products were then purified and sequenced with a 300-cycle paired-end run on a MiSeq Illumina sequencer. 2. Methods for processing the data: Mouse gh ref: GTATTAGGGAACCCCATTCTCCCTTTCCCATAGGCCTTGGGATGTCTCTGGCTGATGAGCTCTTGGCTGACCTCGAGGAGGCAGCAGAAGAAGAGGAAGGAGGAAGCTATGGAGAAGAGGAGGAGGAGCCAGCAATTGAGGATGTACAGGAGGAGACCCAGCTGGATCTTTCTGGAGATTCAGTCAAGAGCATTGCCAAGCTATGGGACAGCAAGATGGTGAGGTGGCACCCTTTAAGTCAGCGTTCCTAGGGAGGAGTGGCCGGAGAGCAGTTCTTTCTGAGAGGCCTAGTTTCTGACTGTGCTCTCCTTCCTCATCAGTTTGCTGAGATCATGATGAAGATTGAGGAGTATATCAGCAAGCAAGCCAACGTGTCGGAAGGTGCCTCCCACCCTCACTTTCCTCTGCCCATTCTGACTCCCCGACTCTCTTGCTCCTTATGGTTTTATCACCAAGTGTC gRNAg, gRNAh GGATGTACAGGAGGAGACCCAG,GCTGAGATCATGATGAAGATTG Mouse gRNAb ref: TTAGGCAGGGAGGTTGTAAAAGTGTAAAAGGAAAGAGAGGCCTCCAGTGTATGGACCTTAGCTTTCCCAGCTTCCTCAAGGGGAGACCTCTGTTGGTGTTGTAGCAAAAGAGGGTCTTTTGGGCTTACTACAAATTCTCCCTCCCCTTCCTACCCCAGGAGCTGGGCAACAGCCTGGATAAGTGCAAGAACAATGAGAACCTCCAGCAGATCCTGACCAATGCTACTATCATGGTTGTTAGTGTCACTGCCTCTACTACCCAGGGGTATGTATGCCAAACGGTAATGTGCCGCAAGCCTACCATAGGACAATTAACAGGAGAGACAGAATGGAGGGCA gRNAb: CCAGGAGCTGGGCAACAGCC Human ab ref: GCATGCTAGTGGGGTTGATAAAGAAGGACCAGGGTCTTCTGGGGGAGAATCATCGCTCAGTAATAAGGAGGGACTTTGTCGGGGCAAGTTTTTAGGGAACGCTGCTGTCCCTCCCCAGGCCTCGGGATGTCTCTGGCAGATGAGCTCTTAGCTGATCTCGAAGAGGCAGCAGAAGAGGAGGAAGGAGGAAGCTATGGGGAGGAAGAAGAGGAGCCAGCGATCGAGGATGTGCAGGAGGAGACACAGCTGGATCTTTCCGGGGATTCAGTCAAGACCATCGCCAAGCTATGGGATAGTAAGATGGTAAGAGGACAAGAGGTGTTCCTAGCAGGGGGCTCTAGACAGAATCTCCCAGAAGGGGGTGATACAGGCTTCTTTTTGAAGAGTGCTGGATTCTGACTGTCT SaCas9 ga, gb: GGATGTGCAGGAGGAGACACAG,TCAAGACCATCGCCAAGCTAT