------------------------------------------------------------------------------------------------------------------------------------------------------------ Title of Dataset: Datasets for: Koussoroplis A, Sperfeld E, Pincebourde S, Bec A, Wacker A. Dietary sterol availability modulates heat tolerance of Daphnia. Freshwater biology ------------------------------------------------------------------------------------------------------------------------------------------------------------- Two datasets are included: Thermal_Tolerance_Data.txt: ----------------------------- This dataset contains heat tolerance (i.e. time to immobilisation) data of Daphnia magna individuals grown for 6 days at 27°C on non-toxic cyanobacterial diet (Synnechococcus elongatus) diets supplemented with different concentrations of Cholesterol (via liposomes). It is used in the supplied R scipts: Figure 1.R - Figure 2.R - Bootstrap.R NT_climatology.txt: -------------------- This dataset contains the average surface water climatology of a northen temperate lakes reported in Woolway et al.(2021). Lake heatwaves under climate change. Nature 589. https://doi.org/10.1038/s41586-020-03119-1. It is used in the supplied R script:Time_series_construction.R ------------------------------------------ Description of the Data and file structure ------------------------------------------ Thermal_Tolerance_Data.txt: ----------------------------- -Temp (explanatory variable): the temperature at which heat tolerance was assayed -P (information, not variable): C:P ratio of Synnechococcus elongatus -EPA (information, not variable): eicosapentaneoic acid concentration (µg EPA / mg C) of the diet (supplemented with liposomes) -Size_mm: (explanatory variable): Size (in mm, top of the head to the basis of the tail spine) of the assayed Daphnia individual -Time_min:(response variable): Time-to-immobilisation (in min, absence of movement and response to physical stimuly for 10 sec) -Time_log: (responsevariable): Time-to-immobilisation, log-transformed. NT_climatology.txt: -------------------- -day_of_yr: day of the year -fraction_of_yr: elapsed fraction of the year -temperature: average daily surface water temperature of an average northern temperate lake