# Title of Dataset: Data: Innate immune function and antioxidant capacity of nestlings of an African raptor co-vary with the level of urbanisation around breeding territories This dataset contains habitat variables, individual bird information and physiological variables measured from Black Sparrowhawks Accipiter melanoleucus in South Africa. Data was used to test how levels of urbanisation influence innate immune function, immune response to a mimicked bacterial infection and antioxidant capacity of nestling Black Sparrowhawks. We also explored the effect of timing of breeding and rainfall on physiology since both can influence the environmental condition under which nestlings are raised. ## Description of the Data and file structure The dataset is divided into nine worksheets each containing headers with a description of the information contained in each analyzed column as a comment. The data from the different worksheets are linked by a unique ring number for each bird and a sampling date for the samples. Lund Samples Petra - oxidative - contains the information of each nestling including, the ID of samples collected, Date of collection, Month when the egg was laid, morph, morph of male and parents, sex, morphometric measurements, ring number and nest identity. sample ID Unique ID for each sample collected in the project No. Serial number of all birds trapped Date Sampled Sampling date lay.month estimated laying month from observation of breeding events h.day Sampling hour min.day Sampling minute week.nr Week number Month Month Year Year Season Season classified as early or late juv.morph Juvenile morph classified as pale or rufous ad.morph Adult morph classified as light or dark (verified subsequently) male.morph Morph of male parent classified as light or dark. female.morph Morph of female parents classified as light or dark pair.morph.1 Grouping of morph of male and female parents as like or mixed pair.morph.2 grouping of morph of male and female parents with the actual morph of each parent (e.g Dark male and light female) sib Code for sibling used for a different project. nb.sib Number of siblings Nest Unique identity of territory where nest is located thawed Whether sampled thawed during transport or not. urban gradient Urban cover within 1500m radius calculated from land cover maps forest Forest cover within 1500m calculated from land cover maps Safring Unique number on metal ring Age Age classified as chick or adult est.ring.age Estimated ringing age Sex Sex survival Whether nestling survived or not Haematocrit Packed cell volume measured from blood collected during sampling. weight Body mass in grams wing Wing length in mm primary.P4 Length of fourth primary feather in mm primary.P8 Length of eight primary feather in mm tail.feather.middle Length of middle tail feather in mm tarsus length of entire tarsus in mm tarsus.bone.length Length of tarsus bone in mm tarsus.long Diameter of tarsus measured with flattened surface up in mm tarsus.lat Diameter of tarsus measured with flattened surface sideways in mm hind.claw Length of hind claw in mm inner.claw Length of inner claw in mm hunger Presence or absence of hunger traces scored from checking for patches of poor feather growth hungertraces.p Number of hunger traces in primary feathers scored from checking for patches of poor feather growth hungertraces.s Number of hunger traces in secondary feathers scored from checking for patches of poor feather growth hungertraces.t Number of hunger traces in tertiary feathers scored from checking for patches of poor feather growth photo quality Feather photo quality scored on the scale of 0 to 3 trap out Time of setting trap bird on trap/chick down Time bird gets trapped or chick get down time bleeding Bleeding time stamp HLHA_20190801 - contains measure of two immune indices from plasma; haemagglutination and haemolysis score on the scale of 0 - 11 and inputted against the unique ring number of each nestling. Safring Unique metal ring number HLHA.agg Haemagglutination score on the scale 0 to 11 HLHA.hem Haemolysis score on the scale of 0 to 11 HP_20190801 - contains estimates of haptoglobin concentration from measures of plasma absorbance at 630, 450 and 405 wavelengths. Additional columns contain, assay plate ID, and immune challenge status against the unique ring number of each nestling. Safring Unique metal ring number challenge Whether lypopolysaccharide injection was adminstered or not plate.ID Assay plate ID Hp.630 Haptoglobin concentration estimated using absorbance measured at 630 wavelength in miligram per mil Hp.630.corr Corrected haptoglobin concentration estimated using absorbance measured at 630 wavelength miligram per mil Hp.405 Sample redness measured at 405 wavelength Hp.450 Sample wavelength measured at 450 wavelength FRAP_20190801 - contains estimates of ferric reducing antioxidant power (FRAP) which measures the overall ferric reducing capacity of plasma and indication of total non-enzymatic antioxidant capacity. Additional columns contain plate ID, absorbance from which FRAP was estimated, the coefficient of variation of FRAP inputted against the unique ring ID of each nestling. Safring Unique metal ring number Plate.ID.FRAP Assay plate ID absorbance.FRAP Absorbance measurement for FRAP assay FRAP Ferric reducing power measurement in micro gram per microliter CV.FRAP Coeficient of variation of FRAP measurement UA_20190801 - contains estimates of Uric Acid concentration in plasma. This measure was used to correct the FRAP measurement (see method section). Additional columns include, Plate ID, absorbance from which Uric Acid concentration was estimated, corrected Uric acid concentration and the coefficient of variation of the Uric Acid concentration. Safring Unique metal ring number Plate.ID.UA Assay plate ID UA.corr Corrected Uric acid measurement in microgram per mil UA Uric acid measurement in microgram per mil UA.absorbance Absorbance measurement for Uric acid assay UA.CV Coefficient of variation of Uric acid measurement BKA_20190801 - contains estimates of bacteria killing capacity of plasma measured as the percentage bacteria killed. Additional columns include corrected estimates and coefficient of variation inputted against the unique ring number of each nestling. Safring Unique metal ring number BKA Bactericidal capacity measure in percentage BKA.corr Corrected bactericidal capacity measure in percentage CV Coefficient of variation of bactericidal capacity measurement GSHGSSG_20190801 - contains estimates of total glutathione content of whole blood with additional columns including the plate ID inputted against the unique ring number of each nestling. Safring Unique metal ring number tGSH Total glutathione concentration in micro mol tGSH.plate Assay plate ID Urban_cover_territory - contains estimate of urban and forest cover calculated within a 2000 m radius surrounding each nest. Urban land cover was based on the South African National land-cover dataset for land cover classes for 2013 - 2014 produced by GEOTERRA (Department of Environmental Affairs, 2015). This comprises 72 land cover types within each 30 m2 land area. Nest Unique identity of territory where nest is located urban gradient1 Urban cover percentage within 2000m radius around nesting tree calculated from land cover maps forest1 Forest cover percentage within 2000m radius around nesting tree calculated from land cover maps Cape town daily data - contains weather data collected from the South African Weather Service weather station located at the Cape Town Airport. Data include, Date, sunshine, maximum humidity, minimum humidity, rainfall, maximum temperature, minimum temperature. Year Year Month Month Day Day Sunshine Sushine intensity kilowatts per square meters Hum Max maximum humidity in percentage Hum Min Minimum humidity in percentage Rainfall Rainfall in mm Tx Maximum temeperature in degree celsius Tn Minimum temperature in degree celcius DATE Date All the missing data are cells are marked 'NA'