This Readme.txt file was generated on 2022-10-31 by Caixia Wei #Title of Dataset: Is Poaceae pollen size a useful proxy in palaeoecological studies? New insights from a Poaceae pollen morphological study in the Amazon ## Author information: 1. Caixia Wei, University of Amsterdam, caixiawei1994@gmail.com, https://orcid.org/0000-0001-5369-944X 2. Jardine, Phillip E., University of Münster, jardine@uni-muenster.de, https://orcid.org/0000-0002-8268-2353 ## Description of the Data and file structure We generated 2540 pollen size data from 127 plant specimens from 58 species across the Poaceae phylogeny from the Amazon drainage basin (ADB). We tested the relationship between pollen size and a range of abiotic and biotic variables (vegetation type, soil composition, climate conditions, photosynthetic pathway and genome size), and fitted models using phylogenetic generalized least squares (P-GLS) and linear mixed models (LMM). ## Data collection of a series abiotic and biotic variables 1. Vegetation type We assigned each sampling locality to one of the following five vegetation types: desert, montane grassland shrubland, savanna, tropical dry forest, and tropical moist forest. 2. Soil type We used the Soil and Terrain Database (SOTER) for Latin America and the Caribbean (SOTERLAC), version 2.0, at a scale of 1:5000000 to define soil categories for each site, using the WRB (World Reference Base) Soil Groups to assign each sampling locality to one of 19 soil types. DOMSOIL_UN represents the soil type corresponding to each sampled plant specimen. 3. Modern climate data We used the WorldClim 2.1 database and Climate Research Unit (CRU) TS4.04 database (Harris et al., 2020) to extract the mean annual temperature. cru.MAT and cru.TAP represents the climate data corresponding to each sampled plant specimen. 4. C3/C4 photosynthetic pathway Photosynthetic pathways assigned to each species came from Klink and Joly (1989), Giraldo-Canas (2010), Bremond et al. (2012), and references therein. 5. Genome size We took genome size information from Kew Garden's C-values Database. #DATA & FILE LIST 1. Pollen_measurements.txt, data file 2. Pollen_metadata.txt, data file 3. Sample_metadata.txt, data file 4. Spriggs_Poaceae_hyp1.tre, phylogenetic tree file 5. Spriggs_Poaceae_hyp2.tre, phylogenetic tree file 6. Poaceae_pollen_size.R, R file 7. README_file.txt, readme file ## Sharing/access Information Links to the publication: https://doi.org/10.1016/j.revpalbo.2022.104790