# ReadMe # Age-specific effects of deletions: implications for ageing theories # Brengdahl et al. Data was analysed using the R-code in "Brengdahl_et_al_code". Data consists of measurements of effects from deletions on life history traits in Drosophila melanogaster (Dahomey population), female fecundity at three ages (day 5, 19 and 33 of adult age), egg-to-adult survival and development time, and survival to adult day 33. In this project the genomic deletions that where used had project names, here is a key between what is in the data files and the real deletion names: Project name: Real name (info also present in the R-code) Alfa: Df(2L)Exel6004 Bravo: Df(2L)Exel6009 Foxtrot: Df(2L)Exel6043 Hotel: Df(2L)Exel6049 Juliette: Df(2R)Exel6057 Kilo: Df(2R)Exel6059 Mike: Df(2R)Exel6063 Oscar: Df(2R)Exel6077 Romeo: Df(3L)Exel6103 Victor: Df(3L)Exel6131 Whiskey: Df(3L)Exel6134 Yankee: Df(3R)Exel6146 Zulu: Df(3R)Exel6180 Epsilon: Df(3R)Exel6191 Theta: Df(3R)Exel6210 Kappa: Df(3R)Exel6218 Lambda: Df(3R)Exel6269 Omega: Df(3R)Exel6288 The file "Fecundity_Main" contains data on female fecundity from the main assay (kept in darkness except when being handled), for 18 deletions and their matched wildtype. The file "Fecundity_Light" contains data on female fecundity from an extra assay (flies were kept on a 12h:12h light/dark schedule), for 2 deletions and their matched wildtype. The file "Development_time" contains data on egg-to-adult development time (and egg-to-adult survival),for 18 deletions and their matched wildtype. This assay was run in constant light. The file "Deletions_surv" contains data on female survival at the times fecundity was scored (day 5, 19 and 33 of adult age), for 18 deletions and their matched wildtype. # Data structure in "Fecundity_Main": "Deletion": Represents the introgressed deletion (using project names). "Vial_ID": Replicate identity within deletion, mutant and wildtype are paired within a vial over time, but separated for measurement of fecundity. Each row in the dataset thus corresponds to one experimental vial. "FX_Mu_X": Represents fecundity (number of eggs laid) for mutant females at age X (1 = day 5, 2 = day 19, 3 = day 33), for cookie X (A or B). Fecundity was measured over a 24 hour period split on two cookies (A and B) for each time point, with ~18h spent on the A cookie and ~6h on the B cookie. "FX_Wt_X":Represents fecundity (number of eggs laid) for wildtype females at age X (1 = day 5, 2 = day 19, 3 = day 33), for cookie X (A or B). Fecundity was measured over a 24 hour period split on two cookies (A and B) for each time point, with ~18h spent on the A cookie and ~6h on the B cookie. # Data structure in "Fecundity_Light": "Deletion": Represents the introgressed deletion (using project names). "Vial_ID": Replicate identity within deletion, mutant and wildtype are paired within a vial over time, but separated for measurement of fecundity. Each row in the dataset thus corresponds to one experimental vial. "FX_Mu_X": Represents fecundity (number of eggs laid) for mutant females at age X (1 = day 5, 2 = day 19, 3 = day 33), for cookie X (A or B). Fecundity was measured over a 24 hour period split on two cookies (A and B) for each time point, with ~18h spent on the A cookie and ~6h on the B cookie. "FX_Wt_X":Represents fecundity (number of eggs laid) for wildtype females at age X (1 = day 5, 2 = day 19, 3 = day 33), for cookie X (A or B). Fecundity was measured over a 24 hour period split on two cookies (A and B) for each time point, with ~18h spent on the A cookie and ~6h on the B cookie. # Data structure in "Development_time" (also used for egg-to-adult survival): "Block": Represents experimental block (5 in total). "Mutation": Represents the introgressed deletion (using project names). "ID": Replicate identity within "Block" and "Mutation", representing the experimental vial a fly developed in. Note that the different genotypes (and sexes) are on separate rows within a vial in this dataset. "Sex": F = Female, M = Male "Genotype": Del = Deletion (mutant), Wt = Wildtype "DayX_XX": The number of emerged flies (since the previous timepoint, checked every 4th hour), where "DayX_" corresponds to day (where "Day1" is the first day of scoring, but corresponds to Day 8 since eggs where laid, Day2 = Day 9, Day3 = Day 10...) and "_XX" corresponds to which hour on a day (24h notation). # Data structure in "Deletions_surv": "Deletion": Represents the introgressed deletion (using project names). "Vial": Replicate identity within deletion, mutant and wildtype are paired within a vial over time, but separated for measurement of fecundity. Each row in the dataset thus corresponds to one experimental vial. "mut_X": Number of mutant (deletion) females present in a vial at timepoint X (1 = day 5, 2 = day 19, 3 = day 33). "wt_X": Number of wildtype females present in a vial at timepoint X (1 = day 5, 2 = day 19, 3 = day 33).