GENERAL INFORMATION 1. Title of Dataset: Data from: Insecticidal activity of Beauveria bassiana against red flour beetle, Tribolium castaneum 2. Author Information Corresponding Investigator 1 Name: Prof. Jae Su Kim Institution: Jeonbuk national university, Jeonju, Korea Email: jskim10@jbnu.ac.kr Corresponding Investigator 2 Name: Prof. Yasuyuki Arakane Institution: Chonnam national university, Gwangju, Korea Email: arakane@chonnam.ac.kr Co-investigator 1 Name: Sehyeon Beak Institution: Jeonbuk national university, Jeonju, Korea Co-investigator 2 Name: Prof. Mi Young Noh Institution: Chonnam national university, Gwangju, Korea Co-investigator 3 Name: Seulgi Mun Institution: Chonnam national university, Gwangju, Korea Co-investigator 4 Name: Prof. Se Jin Lee Institution: Sunchon national university, Suncheon, Korea 3. Date of data collection: 2019-2022 4. Geographic location of data collection: Jeonju and Gwangju in Korea 5. Funding sources that supported the collection of the data: Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ015927) and the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (NRF-2021R1A2C1006645) DATA & FILE OVERVIEW 1. File List: File 1 Name: Bioassay raw data for figure 1.xlsx DATA-SPECIFIC INFORMATION FOR: Bioassay raw data for figure 1.xlsx 1. Number of variables: 4 2. Number of cases/rows: 73 3. Variable List: Treatment: Larva Control, Larva Bb, Adult Control, Adult Bb Time (h): time after treatment; 0, 24, 36, 48, 60, 72 Rep: Replicate; 3 live: Number of alive Tribolium castaneum 4. Missing data codes: None 5. Abbreviations used: Larva Control; Non-treated Tribolium castaneum larva, Larva Bb; Beauveria bassiana ERL1170-egfp-treated Tribolium castaneum larva, Adult Control; Non-treated Tribolium castaneum adult, Adult Bb; Beauveria bassiana ERL1170-egfp-treated Tribolium castaneum adult 6. Other relevant information: Larvae or mature adults (n = 20) were placed on a cultured plate for 10 min to expose them to the fungal conidia. The treated insects were transferred to a fresh plate with a layer of wet filter paper (200 μL of distilled water) and approximately 0.3 g of wheat flour was added to the plate as feed. Three replicates were applied as a treatment. Non-treated beetles served as a control, with the beetles being placed on the non-cultured agar plates. All the plates were kept in a plastic cage (30 × 20 × 15 cm3), which was layered with wet paper towels receiving approximately 10 mL of distilled water to achieve high humidity conditions. The plastic cage was covered with a lid and kept at a room temperature of 24 ± 2 °C for 5 days. Living and dead beetles were counted daily.