# README # Images and raw data for the manuscript: "Dissecting the genetic architecture of leaf morphology traits in Mungbean (Vigna radiata (L) Wizcek) using genome-wide association study" --- Leaves were collected from one replication per location for the two years giving us four data points. Leaf collections were done during the vegetative growth and took between three to five days at each location, weather permitting and the availability of labor. Leaves were collected on the following dates in 2020: Burkey 2-5 September, Bruner 7, 13-15 September, and 2021: AEA 27-28 July, 9-11 August, Bruner 11-13, 16-17 August. The third trifoliate leaf from the top (most recent bud) on the plant was plucked destructively as the leaf below was already senescing in some plants and already dropped in others. The third leaf represented the mature leaf on the plant. Three trifoliate were collected randomly per plot, put in a Ziploc, and temporarily stored in a cooler box. The cooler box was later transported to the imaging station. ## Description of the Data and file structure Data includes annotated images and raw trait data extraited in excel sheet. In the test_traits.xls the NA represents missing values from the extraction pipeline. This could be caused due to inability of extraction software to extract the traits. annotated_leaves.zip |- images/ | |-2020/ | |-2021/ |- raw_data/ | |2020/ | |2021/ | |...csv |- README.md ### Description of the leaf traits used in the study. | Trait | Description | Unit | | --------- | -------------------------------------------------------------------------------------- | ---- | | Length | The distance from proximal-most to distal-most point of the lamina | cm | | Width | The distance across the laminar that lies perpendicular to the axis of greatest length | cm | | Perimeter | The distance around the margin of the laminar | cm | | Area | Laminar size | cm2 | | Apex angle | The angle from the apical termination of the midvein to the pair of points where a line perpendicular to the midvein and 0.75 x length from the base intersects the margin | Degrees (o) | | Base angle | The angle from the leaflet base to the pair of points where aline perpendicular to the midvein and 0.25 x length from the base intersects the margin | Degrees (o) | | | *Traits are prefixed with a letter indicating the leaflet. i.e., l_(left), t_(terminal), r_(right) | | ### Extras names/acronymns and meaning | Name | Description | | -------- | ---------------------------------------------------- | | Centroid | Cordinates of the center of the leaflet | | fname | Name of the leaflet sample | | loc | Location | | bloc | Block in the Randomized Complete Block Design (RCBD) | | n | Sample (leaflet) number | ## Sharing/access Information ## Code/Software Any software that can view images You can extract the data using the open-source software at https://bitbucket.org/baskargroup/leaf_annotator/src/master/ The analysis code can be accessed at https://github.com/yalek/mungbean_leaf_gwas