Archived data for: Rapid evolution caused by pollinator loss in Mimulus guttatus by Sarah A. Bodbyl Roels and John K. Kelly Description of phenotypic data for Generations 6 and 7. Generation 6 was a "parent" generation grown without selection after five consecutive generations of evolution under our bumblebee pollinator and no pollinator treatments. Generation 7 was the progeny of fully random, within population crosses of the generation 6 plants. Populations: A1 and A2 are replicate populations that were maintained under no pollinator treatments. No Bee B1 and B2 are replicate populations that received pollination services by Bombus impatiens bumblebees. Bee Source: the original source population for the replicated, evolved populations. Individuals grown concurrent with Generation 6 evolved populations were also randomly crossed and grown again along with Generation 7 plants for comparisons. File: Generation 6 phenotypes.txt Explanation of variables: ID: Identifies individual plants within each population CW 1: Corolla width of the first flower (mm) TW 1: Throat width of first flower (mm) TL 1: Tube length of first flower (mm) A/S 1: Anther-stigma separation measured on first flower (mm) 2's: Same measures made on second flowers (all in mm) Leaf W: Maximum width of the largest leaf on the plant at the time of the second flower measurement (mm) Avg's: Average measures from the first two flowers. POLL: Coulter particle counter measure of total amount of the average floral pollen in a sample (from the third flower produced, two samples analyzed) fPoll: Adjusted amount of pollen for an entire flower (=POLL*20) ln fpoll: Natural log of fPoll PSI: Pollen size index (derived from Coulter counter measures of viable and inviable pollen grain sizes) PV: Pollen viability (derived from Coulter counter measures) File: Generation 7 phenotypes.txt Explanation of variables: COH: Cohort. This generation was grown in two consecutive cohorts. REP: Replicate. This generation had three replicates from each family produced by Generation 6 crosses. Dams: The Generation 6 ID of the pollen recipient that produced the plant. Sire: The Generation 6 ID of the pollen donor that produced the plant. dayf: Number of experiment days (planting = day 0) until the first flower CW, TW, TL, A/S: Same as for Generation 6 file Leaf: Leaf width (same as Generation 6 file) Nsseed: Raw count of the number of self-fertilized seeds produced by an unmanipulated fourth flower produced by the plant. POLL, fpoll, PSI, PV: Same as above in Generation 6 File: Generation 6 Gene-based markers.txt The first panel is a summary of observed heterozygosity (Het) and total number (n) for each population at four putatively neutral gene-based markers used in the study (MgSTS_461, MgSTS_755, MgSTS_523, and MgSTS_641). The second panel is a list of the calls (either allele size--e.g. 316, or presence/absence--e.g. 0 or 1) made for each individual plant genotyped. File: Generation 6 polymorphism counts.txt The first two panels are genotype counts for the two chromosomal polymorphisms genotyped in this study. The length polymorphic marker HB5 was used as an indicator of the genotype at the meiotic drive polymorphism (D/d). The length of the HB5 allele is 277 (276 with our program calls) base pairs (bp) at the derived locus classification, D, and all other allele lengths were scored as the ancestral allele, d. The derived inversion haplotype, C, was identified by a combination of alleles at two marker loci: allele length 240 at marker locus MgSTS_431 and allele length 201 at MgSTS_229. All other combinations were scored c (ancestral). q(derived) is the frequency of the derived polymorphism in the population. The following section is the full genotype scoring record for each individual. Dcount is 0 (no D), 1(heterozygote), 2 (homozygous D). C2count combines calls from e229 and e431 making 0 (no C), 1(heterozygote), 2 (homozygous C). Individuals with incomplete genotype information at either locus were not scored for C2count. File: Fitness and Inbreeding Estimates.txt The first section gives the seed mass (g) produces in bulk by all individuals of each population (A1, A2, B1, B2) for the five generations of treatment. The second section gives the number of individuals counted in each population for each of the five generations. The third panel gives estimated F (inbreeding coefficients) for each of the populations (A1, A2, B1, B2, Source). We used the moment estimator for F of Lynch and Ritland (1999) calculated by the program COANCESTRY (Wang 2010); http://www.zsl.org/science/research/software). F of each population was estimated from genotypic data (Generation 6 plants) at the length polymorphic markers MgSTS_461, MgSTS_755, MgSTS_523, and MgSTS_641. (See File: Generation 6 Gene-based markers.txt)