#Reference Information ##Provenance for this README File name: README.md Authors: YangPing, Other contributors: Qi Gao, Changxu Li, Yan Wang, Yuliang Wang, Shuo Li, Mingjing Qiu, Linqian Zhang, Ailing Tu, Yu Tian, Hong Zhao Date created: 2023-02-09 #Dataset Attribution Dataset Title: Data for the article“Construction of Microneedle of Atractylodes macrocephala Aqueous Extract and Effect on Mammary gland hyperplasia Based on Intestinal Flora” Dataset Contributors: Creators: Yang Ping, Qi Gao, Changxu Li, Yan Wang, Yuliang Wang, Shuo Li, Mingjing Qiu, Linqian Zhang, Ailing Tu, Yu Tian, Hong Zhao Publisher:Jiamusi University #Contact Information Name: YangPing Affiliations: College of Pharmacy, Jiamusi University, Jiamusi,Heilongjiang, China Email: 18245482328@163.com #Additional Dataset Metadata ##Acknowledgements This study was supported by National innovation and entrepreneurship training program (202210222135), Basic Scientific Research Funds for Provincial Colleges and Universities in Heilongjiang Province(Construction of percutaneous physical permeability promoting microneedle patch based on acupoint administration), Basic Scientific Research Funds for Provincial Colleges and Universities in Heilongjiang Province(Study on the mechanism of Atractylodes macrocephala grading polysaccharide against breast hyperplasia), Jiamusi University Ph.D. Special Foundation (JMSUBZ2020-14). #Methodological Information Methods of data collection/generation: see manuscript for details #Data and File Overview ##Summary Metrics File count: 6 Total file size: 263 KB Range of individual file sizes: 10KB - 250 KB File formats: .xlsx #Table of Contents Length of the sequence.xlsx alpha diversity.xlsx OTU.xlsx Phylum genus level.xlsx serum indicators.xlsx beta diversity.xlsx #File/Folder Details ##Details for: Length of the sequence.xlsx Description: After taking blood from the abdominal aorta, the rats were quickly executed by cervical dislocation. Under aseptic conditions, the caecal content was taken with sterilized forceps and placed in a 5 mL sterile EP tube, numbered, weighed, and quickly placed in liquid nitrogen. After the sample is collected, move it to - 80 ℃ for storage. Take the caecal content. The total DNA in content was extracted in strict accordance with the DNA extraction kit. Nanodrop spectrophotometer was utilized to quantify DNA (DNA quantitative analysis), and the purity and concentration of the extracted genomic DNA were identified by electrophoresis. Amplification was performed using the 16S rDNA V3-V4 variable region, and the Quant-it PicoGreen dsDNA Assay Kit was used for fluorescence quantification. For further fluorescence quantification, samples should be combined in the appropriate proportions. Afterward, Illumina's NovaSeq 6000 sequencer was utilized for double-end sequencing of rat caecal contents. Format(s): .xlsx Size(s):12KB Dimensions:90 rows x2 columns Variables: Length of the sequence:the coding length of DNA genes. The size of gene molecules in the range of 400-500bp is moderate and the mutation rate is small, which is the most commonly used and useful marker in the taxonomy of bacterial systems. Count:the intestinal contents microbiota ##Details for: alpha diversity.xlsx Description: Alpha diversity indices (Chao1, ACE, Simpson and Shannon indices) were calculated to obtain the species richness and diversity. Format(s): .xlsx Size(s):11KB Dimensions:10rows x 6columns Variables: SampleID: Con1, Con2, Con3, AM-DMN1, AM-DMN 2, AM-DMN 3, Mod1, Mod2, Mod3 Group: Con group, AM-DMN group, Mod group Chao1: Chao1 indice is often used to estimate the total number of specie in a community Pielou: Pielou indice is often used to estimate the total number of species in a community Simpson: Simpson indice can take into account both species richness and evenness, and they provide an objective picture of community species diversity. Shannon: Shannon indice can take into account both species richness and evenness, and they provide an objective picture of community species diversity. Con: blank control group AM-DMN: Atractylodes Macrocephala Rhizoma microneedle group Mod: model control group ##Details for: OTU.xlsx Description:OTU clustering of non-repetitive sequences was made with 97% similarity using QIIME2 DADA2 software. Species classification was annotated using the Greensenes database (release 13.8). Format(s): .xlsx Size(s):203KB Dimensions:1835rows x 3columns Variables: Group: Con group, AM-DMN group, Mod group Con: blank control group AM-DMN: Atractylodes Macrocephala Rhizoma microneedle group Mod: model control group ##Details for: Phylum genus level.xlsx Description: Indicates the change of flora abundance in each sample at the level of phylum and genus. Format(s): .xlsx Size(s):17KB Variables: The file contains three subdirectories. Directory A:(phylum level-1) Con: blank control Mod: model control AM-DMN: Atractylodes Macrocephala Rhizoma microneedle group Directory B:(phylum level-2) Con1: No. 1 blank control Con2: No. 2 blank control Con3: No. 3 blank control AM-DMN1:No.1 Atractylodes Macrocephala Rhizoma microneedle group1 AM-DMN2:No.2 Atractylodes Macrocephala Rhizoma microneedle group2 AM-DMN3:No.3 Atractylodes Macrocephala Rhizoma microneedle group3 Mod1: No.1model control Mod2: No.2model control Mod3: No.3model control Directory C:(genus level) Con1: No. 1 blank control Con2: No. 2 blank control Con3: No. 3 blank control Mod1: No.1model control Mod2: No.2model control Mod3: No.3model control AM-DMN1:No.1 Atractylodes Macrocephala Rhizoma microneedle group1 AM-DMN2:No.2 Atractylodes Macrocephala Rhizoma microneedle group2 AM-DMN3:No.3 Atractylodes Macrocephala Rhizoma microneedle group3 ##Details for: serum indicators.xlsx Description:After the last administration, rats in each group fasted for 12h. After weighing, rats were anesthetized by intraperitoneal injection of 2% pentobarbital sodium. The rats were done by cervical dislocation. Blood was drawn from the abdominal aorta and placed in the procoagulant tube for 20min to separate the serum. The serum levels of E2, P and PRL were identified by ELISA. Format(s): .xlsx Size(s):13KB Dimensions:19rows x 2columns Variables: Group: Con group, AM-DMN group, Mod group, there were 6 rats in each group. Content(pmol/L): The content of detection indexes (E2, P and PRL) in rat serum samples. ##Details for: beta diversity.xlsx Description: Meanwhile, the Beta diversity was analysed in the gut microbiota of different samples, the similarity and difference of community composition between samples (or subpopulations) were obtained by analyzing the Beta diversity of gut microbiota in different samples. Format(s): .xlsx Size(s):11KB Variables: The file contains two subdirectories. Directory A:(PCA) Sample ID: Con1, Con2, Con3, AM-DMN1, AM-DMN2, AM-DMN3, Mod1, Mod2, Mod3 Directory B:(NMDS) taxonomy: it represents the name of the genus analyzed.