There were seven analyses performed for this paper and the files in the depository are grouped by analysis as outlined below: >Survival and Development Atomentosus.survival burk=0 Burkholderia-negative; burk=1 Burkholderia-positive censor.status=1 death date is known, occurred before end of expt. censor.status=0 insect was still alive at end of expt. molt.times.csv time = # days until insect reached next instar (not cumulative) surv&dev.R R script >16S Phylogeny 16Sphylogeny.nex Greengenes-aligned sequences in nexus format >MLST Phylogeny mlst.phylogeny.nex genes concatentated and aligned in this order: atpD, gltB, lepA, recA atpD.fasta full atpD sequences for samples in this study gltB.fasta full gltB sequences for samples in this study lepA.fasta full lepA sequences for samples in this study recA.fasta full recA sequences for samples in this study >PERMANOVA This analysis was used to test for the significance of species and location in determining the presence and absence of OTUs in each sample using R. There is not a column of sample in the sample x OTU matrix, but they are found in the vars file. Files: GAbugs.clones.dichotomous.csv sample (rows) x OTU (columns) matrix vars.csv species and location for each row in the sample x OTU matrix adonis.R R script that uses the adonis function from vegan >AMOVA GAubgclones.fasta cloned sequences from GA insects only processed in the same way as in mothur.code.txt (heatmap section) GAbugclones.locations.groups.txt location identifier for each sample (design file) GAbugclones.species.groups.txt species identifier for each sample (design file) AMOVA.code.txt mothur script >Heatmap The abundance and distribution of the OTUs (defined at 0.03 dissimilarity) present in each insect species presented as a heatmap made using mothur. Files: bugclones.enviroisolates.fasta aligned sequences from this study & others bugclones.enviroisolates.species.groups.txt .groups file required to make heatmap mothur.code.txt mothur script >ZOI assays Three zone of inhibition experiments were performed: 1) resistance of Burkholderia sp. 11BH497 and E. coli to immune-challenged (E. coli LPS treatment) and no-stab control hemolymph from A. conspersus; 2) resistance of Burkholderia sp. 11BH497 and E. coli to hemolymph to two different immune elicitors (E. coli LPS and heat-killed Burkholderia) from Alydus spp.; and 3) resistance of five additional bacteria to immune-challenged (E. coli LPS) hemolymph from Alydus spp. Files: ZOI.Aconspersus.csv experiment 1 ZOI.2elicitors.csv experiment 2 ZOI.multiplebac.csv experiment 3