******************************************************* INTRODUCTION ******************************************************* "First field-based evidence that the seagrass-lucinid mutualism can mitigate stress in seagrasses" Matthijs van der Geest, Tjisse van der Heide, Marianne Holmer, Rutger de Wit VERSION 31-01-2020 This ReadMe file describes the data files accompanying the above publication. For any further queries please contact: matt.vandergeest@gmail.com ******************************************************* CONTENTS ******************************************************* This data package includes the following files: DATA FILES BENTHIC SURVEY: * "Table_1_Benthic_survey_coordinates_sampling_stations.txt" GPS-coordinates of the stations where benthic cores and pore water samples were taken for our benthic survey in Thau lagoon (France) during April-May 2016. * "Table_2_Benthic_survey_seagrass_lucinid_data_per_sampling_station.txt" Raw data on seagrass above-ground (g DW per benthic core), seagrass below-ground (g DW per core), and Loripes orbiculatus numerical density (ind per core) As our benthic core had a diameter of 100 mm, these raw data need to be divided by 0.00785 to get values per m2 as used in the paper. * "Table_3_Benthic_survey_Loripes_orbiculatus_clam_length_in_mm_per_sampling_station.txt" Raw data on shell length (mm) of Loripes orbiculatus clams sampled per benthic core (100 mm diameter) for each sampling station in Thau lagoon * "Table_4_Benthic_survey_porewater_sulfide_per_sampling_station.txt" Raw data on shell length (mm) of Loripes orbiculatus clams sampled per benthic core (100 mm diameter) for each sampling station in Thau lagoon DATA FILES FIELD EXPERIMENT: * "Table_5_Field_experiment_coordinates_blocks.txt" GPS-coordinates of the blocks used in our field experiment in Thau lagoon (France) during June-August 2016. * "Table_6_Field_experiment_initial_sediment_conditions_per_block.txt" Raw data on sediment mean grains-size (micrometer), sediment organic content (%), sediment carbonate content (%), and sediment iron content (micromol per g) for each block at the start of the field experiment. * "Table_7_Field_experiment_porewater_nutrient_and_sulfide_concentrations.txt" Raw data on pore water sulfide (micromol per L), NO3 (micromol per L), NH4 (micromol_per_L), and PO4 (micromol per L) concentration for each experimental plot at the end of the field experiment. Data for NO3 and NH4 were summed up per experimental plot, to obtain pore water "Total N" concentration per experimental plot, which was used for further analysis in the paper. * "Table_8_Field_experiment_seawater_d34S_values.txt" Raw data on d34S (pro mille) signatures of seawater sampled the end of the field experiment * "Table_9_Field_experiment_Z_noltei_AND_L_orbiculatus_data_per_experimental_plot.txt" Raw data on Zostera noltei shoot density (nr. per core), Z. noltei leaf biomass (g DW per core), Z. noltei rhizome biomass (g DW per core), Z. noltei root biomass (g DW per core), Loripes orbiculatus numerical density (ind. per core), L. orbiculatus flesh total biomass (DW g per core), d34S (pro mille) signature for the sediment CRS-pool, d34S (pro mille) signature for Z. noltei apical leaf tissues, total sulfur content (%) of Z. noltei apical leaves tissues, Sedimentary sulfide intrusion (%) into Z. noltei apical leaves tissues, and the amount of sulfur incorporation (% of dry weight) into Z. noltei leaves from sedimentary sulfides for each experimental plot at the end of the field experiment. As our benthic core had a diameter of 100 mm, raw data expressed per core need to be divided by 0.00785 to get values per m2 as used in the paper. * "Table_10_Field_experiment_L_orbiculatus_data_per_individual.txt" Raw data on shell length (mm), flesh dry weight (g DW), shell dry weight (g DW), and condition (expressed as flesh dry weight/shell dry weight) for each individual Loripes orbiculatus clam sampled per core (100 mm diameter) at each experimental plot at the end of the field experiment. Note that there are 42 missing values (indicated as "NA") for "Shell_DW_g" and as a consequence also for "Loripes_condition". This is because in some occasions the shell of L. orbiculatus was broken due to the removal of flesh tissues from the shell tissues, so that we could not accurately determine shell dry weight for these individuals. * "Table_11_Field_experiment_L_orbiculatus_isotope_data_per_individual.txt" Raw data on shell length (mm), d34S (pro mille) signature of flesh tissues, and total sulfur content (%) of flesh tissues per individual Loripes orbiculatus clam sampled per experimental plot at the end of the field experiment. ******************************************************* DATA DESCRIPTION ******************************************************* Eleven seperate data files are available of which the first 4 date files are concerning the benthic survey and the remaining 7 data files are concerning the field experiment both in Thau lagoon, France. DATA DESCRIPTION BENTHIC SURVEY: Named variables in the data file "Table_1_Benthic_survey_coordinates_sampling_stations.txt" are defined as follows: "Site" (TEXT) - ID of Site "Station" (TEXT) - ID of sampling station within Site "Latitude" (NUMERIC) - Latitude in decimal degrees (DD) of the location of the sampling station "Longitude" (NUMERIC) - Longitude in decimal degrees (DD) of the location of the sampling station Named variables in the data file "Table_2_Benthic_survey_seagrass_lucinid_data_per_sampling_station.txt" are defined as follows: "Site" (TEXT) - ID of Site "Station" (TEXT) - ID of sampling station within Site "Date_sampled" (TEXT) - Date when the benthic core (100 mm diameter) was taken "Seagrass_aboveground_g_DW_per_core" (NUMERIC) - Dry weight (gram) of seagrass above-ground (leaf) tissues per benthic core "Seagrass_belowground_g_DW_per_core" (NUMERIC) - Dry weight (gram) of seagrass below-ground (roots+rhizomes) tissues per benthic core "Loripes_orbiculatus_density_per_core" (NUMERIC) - Numerical density of Loripes orbiculatus clams per benthic core Named variables in the data file "Table_3_Benthic_survey_Loripes_orbiculatus_clam_length_in_mm_per_sampling_station.txt" are defined as follows: "Site" (TEXT) - ID of Site "Station" (TEXT) - ID of sampling station within Site "Date_sampled" (TEXT) - Date when the benthic core (100 mm diameter) was taken "Species" (TEXT) - Full name of the species "Shell_length_mm" (NUMERIC) - Length (mm) of Loripes orbiculatus clams Named variables in the data file "Table_4_Benthic_survey_porewater_sulfide_per_sampling_station.txt" are defined as follows: "Site" (TEXT) - ID of Site "Station" (TEXT) - ID of sampling station within Site "Date_sampled" (TEXT) - Date when the pore water sample was taken "Sulfide_micromol" (NUMERIC) - Pore water sulfide concentration (micromol per L) DATA DESCRIPTION FIELD EXPERIMENT: Named variables in the data file "Table_5_Field_experiment_coordinates_blocks.txt" are defined as follows: "Site" (TEXT) - ID of Site "Block" (TEXT) - ID of experimental block "Latitude" (NUMERIC) - Latitude in decimal degrees (DD) determined for the middle of the experimental block "Longitude" (NUMERIC) - Longitude in decimal degrees (DD) determined for the middle of the experimental block Named variables in the data file "Table_6_Field_experiment_initial_sediment_conditions_per_block.txt" defined as follows: "Site" (TEXT) - ID of Site "Block" (TEXT) - ID of experimental block "Date_sampled" (TEXT) - Date when the benthic core (100 mm diameter) was taken in the middle of each experimental block "Mean_grain_size" (NUMERIC) - Sediment mean grain size (in micrometers) "Organic_content_perc" (NUMERIC) - Sediment organic content (%) "Carbonate_content_perc" (NUMERIC) - Sediment carbonate content (%), "Iron_content_micromol_per_g" (NUMERIC) - sediment iron content (micromol per g) Named variables in the data file "Table_7_Field_experiment_porewater_nutrient_and_sulfide_concentrations.txt" defined as follows: "Site" (TEXT) - ID of Site "Block" (TEXT) - ID of experimental block "Experimental_group" (TEXT) - Label for each experimental group per block (C=Control, L=addition of Loripes orbiculatus, OM=addition of organic matter, OM_L=addition of both L. orbiculatus and organic matter "Plot_ID" (TEXT) - ID of experimental plot per block "OM_addition" (TEXT) - In this column "C" refers to plots to which no organic matter is added (C and L-plots), while "OM" refers to plots to which we did add organic matter (OM and OM_L-plots) "Loripes_addition" (TEXT) - In this column "C" refers to plots to which no Loripes orbiculatus clams are added (C and OM-plots), while "L" refers to plots to which we did add Loripes orbiculatus clams (L and OM_L-plots) "Date_sampled" (TEXT) - Date when the benthic core (100 mm diameter) was taken in the middle of each experimental block "Sulfide_micromol_per_L" (NUMERIC) - Pore water sulfide concentration (micromol per L) "NO3_micromol_per_L" (NUMERIC) - Pore water nitrate concentration (micromol per L) "NH4_micromol_per_L" (NUMERIC) - Pore water ammonium concentration (micromol per L) "PO4_micromol_per_L" (NUMERIC) - Pore water phosphate concentration (micromol per L) Named variables in the data file "Table_8_Field_experiment_seawater_d34S_values.txt"defined as follows: "Site" (TEXT) - ID of Site "Block" (TEXT) - ID of experimental block "Date_sampled" (TEXT) - Date when the seawater sample was taken "d34S_seawater" (NUMERIC) - d34S signature (per mille) for sampled seawater Named variables in the data file "Table_9_Field_experiment_Z_noltei_AND_L_orbiculatus_data_per_experimental_plot.txt" defined as follows: "Site" (TEXT) - ID of Site "Block" (TEXT) - ID of experimental block "Experimental_group" (TEXT) - Label for each experimental group per block (C=Control, L=addition of Loripes orbiculatus, OM=addition of organic matter, OM_L=addition of both L. orbiculatus and organic matter "Plot_ID" (TEXT) - ID of experimental plot per block "OM_addition" (TEXT) - In this column "C" refers to plots to which no organic matter is added (C and L-plots), while "OM" refers to plots to which we did add organic matter (OM and OM_L-plots) "Loripes_addition" (TEXT) - In this column "C" refers to plots to which no Loripes orbiculatus clams are added (C and OM-plots), while "L" refers to plots to which we did add Loripes orbiculatus clams (L and OM_L-plots) "Date_sampled" (TEXT) - Date when the benthic core (100 mm diameter) was taken in the middle of each experimental plot "Zn_shoot_density_per_core" (NUMERIC) - Zostera noltei shoot density (nr. per benthic core) "Zn_leaf_DW_g_per_core" (NUMERIC) - Zostera noltei leaf biomass (gram dry weight per benthic core) "Zn_rhizome_DW_g_per_core" (NUMERIC) - Zostera noltei rhizome biomass (gram dry weight per benthic core) "Zn_root_DW_g_per_core" (NUMERIC) - Zostera noltei root biomass (gram dry weight per benthic core) "Loripes_density_per_core" (NUMERIC) - Loripes orbiculatus numerical density (ind. per benthic core) "Loripes_total_DW_g_per_core" (NUMERIC) - L. orbiculatus flesh total biomass (gram dry weight per benthic core) "d34S_CRS_sediment" (NUMERIC) - d34S (pro mille) signature for the sediment CRS-pool "d34S_leaves" (NUMERIC) - d34S (pro mille) signature for Zostera noltei apical leaf tissues "TS_leaves" (NUMERIC) - Total sulfur content (%) of Zostera noltei apical leaves tissues "Fsulfide" (NUMERIC) - Sedimentary sulfide intrusion (%) into Zostera noltei apical leaves tissues "SS_leaves" (NUMERIC) - The amount of sulfur incorporation (% of dry weight) into Zostera noltei leaves from sedimentary sulfides Named variables in the data file "Table_10_Field_experiment_L_orbiculatus_data_per_individual.txt" defined as follows: "Site" (TEXT) - ID of Site "Block" (TEXT) - ID of experimental block "Experimental_group" (TEXT) - Label for each experimental group per block (C=Control, L=addition of Loripes orbiculatus, OM=addition of organic matter, OM_L=addition of both L. orbiculatus and organic matter "Plot_ID" (TEXT) - ID of experimental plot per block "OM_addition" (TEXT) - In this column "C" refers to plots to which no organic matter is added (C and L-plots), while "OM" refers to plots to which we did add organic matter (OM and OM_L-plots) "Loripes_addition" (TEXT) - In this column "C" refers to plots to which no Loripes orbiculatus clams are added (C and OM-plots), while "L" refers to plots to which we did add Loripes orbiculatus clams (L and OM_L-plots) "Date_sampled" (TEXT) - Date when the benthic core (100 mm diameter) was taken in the middle of each experimental plot "Species" (TEXT) - Full name of the species "Clam_ID" (NUMERIC) - ID for each individual Loripes orbiculatus clam "Shell_length_mm" (NUMERIC) - Length (mm) of Loripes orbiculatus clam "Flesh_DW_g" (NUMERIC) - Loripes orbiculatus flesh biomass (gram dry weight). "Shell_DW_g" (NUMERIC) - Loripes orbiculatus shell biomass (gram dry weight). Note that there are 42 missing values (indicated as "NA"). "Loripes_condition" (NUMERIC) - Loripes orbiculatus condition expressed as Flesh_DW_g/Shell_DW_g ratio. Note that there are 42 missing values (indicated as "NA"). Named variables in the data file "Table_11_Field_experiment_L_orbiculatus_isotope_data_per_individual.txt" defined as follows: "Site" (TEXT) - ID of Site "Block" (TEXT) - ID of experimental block "Experimental_group" (TEXT) - Label for each experimental group per block (C=Control, L=addition of Loripes orbiculatus, OM=addition of organic matter, OM_L=addition of both L. orbiculatus and organic matter "Plot_ID" (TEXT) - ID of experimental plot per block "OM_addition" (TEXT) - In this column "C" refers to plots to which no organic matter is added (C and L-plots), while "OM" refers to plots to which we did add organic matter (OM and OM_L-plots) "Loripes_addition" (TEXT) - In this column "C" refers to plots to which no Loripes orbiculatus clams are added (C and OM-plots), while "L" refers to plots to which we did add Loripes orbiculatus clams (L and OM_L-plots) "Date_sampled" (TEXT) - Date when the benthic core (100 mm diameter) was taken in the middle of each experimental plot "Species" (TEXT) - Full name of the species "Clam_ID" (NUMERIC) - ID for each individual Loripes orbiculatus clam "Shell_length_mm" (NUMERIC) - Length (mm) of Loripes orbiculatus clam "d34S_Loripes_flesh" (NUMERIC) - d34S (pro mille) signature for Loripes orbiculatus flesh tissues "TS_Loripes_flesh" (NUMERIC) - Total sulfur content (%) of Loripes orbiculatus flesh tissue