The data file provided is associated with the article published by Burton et al in Proceedings of the Royal Society Series B: "Acclimation capacity and rate change through life in the zooplankton Daphnia". The file contains 2 data sheets: "Burton2020_ProcB_HeatTolerance.csv","Burton2020_ProcB_TrackingData.csv", that can be used to peform the analyses contained in the paper. The variable names in each of these data sheets are explained below. The data file also contains an R script: "Burton2020_ProcB_HeatToleranceCalculation&Analysis.R" The script describes (i) how to calculate heat tolerance (i.e. time until immobilization, Timm) from the tracking data contained in "Burton2020_ProcB_TrackingData.csv" and (ii) how to perform the analyses of acclimation capacity and rate contained in the paper. 1. Burton2020_ProcB_HeatTolerance.csv: heat tolerance data (i.e. time until immobilization, Timm) as analysed in the paper # ID : integer, numeric value identifying each individual measured for Timm # Timm : numeric, time to immobility (in seconds) at 37 deg C for each Daphnid # Arena : integer, 1-30, arena in which a given individual was located during measurement of Timm # Run : integer, 1 - 23, identfier for each measuring run # Treatment : Factor, w/ 4 levels "17","17to28","28","28to17", describing each acclimation treatment # Jar : integer, 16 values, one for each jar used to rear animals # TreatmentDuration: numeric, duration of hardening treatment (in hours) experienced by individuals in each run # Length : numeric, body size of each individual measured for Timm (in mm) 2. Burton2020_ProcB_TrackingData.csv: tracking data used to calculate heat tolerance (i.e. time until immobilization, Timm) of Daphnia from the respective acclimation treatments, as presented in (1) above. To be used in conjunction with the R script (3) below. # ID : integer, numeric value identifying each individual measured for Timm # Arena : integer, 1-30, arena in which a given individual was located during measurement of Timm # Run : integer, 1 - 23, identfier for each measuring run # Treatment : Factor, w/ 4 levels "17","17to28","28","28to17", describing each acclimation treatment # Jar : integer, 16 values, one for each jar used to rear animals # TimeinArena : integer, time (in seconds)) elapsed after placement of 1st individual in a given measuring run into the well-plate. Thus, 1st individual placed in well plate always has 'TimeinArena' = 0 sec # CameraStart : integer, describing the time (in seconds) when video recording was initiated in each measuring run, is expressed relative to 'TimeinArena'. Thus 'CameraStart' will always > the largest value for 'TimeinArena' in each measuring run # TreatmentDuration: numeric, duration of hardening treatment (in hours) experienced by individuals in each run # Length : numeric, body size of each individual measured for Timm (in mm) # Time : numeric, time series value (in seconds) corresponding to each measurment of 'Velocity' # Velocity : numeric, swimming speed (mm per second) of each individual for at each value of 'Time' # Date : integer, date on which individual was measured 3. Burton2020_ProcB_HeatToleranceCalculation&Analysis.R