This package contains source data and real-time deformability cytometry (RT-DC) raw data as used for publication in Toepfner, N. et al. ################################ CONTENT ########################################## Data.xlsx contains all analyzed data sorted in sheets according to the figures and figure supplements. Data.zip contains the respective RT-DC datasets in hierachical folder structures sorted according to the figures of the publication. The zip archive is split in 31 parts of 990 MB, all necessary in the extraction process. Copressed and split using 7-zip. ################################ RT-DC datasets ################################### RT-DC datasets can be opened and analyzed using ShapeOut - an open source tool. ShapeOut is available at https://github.com/ZELLMECHANIK-DRESDEN/ShapeOut/releases One RT-DC dataset consists of a set of files all starting with an identifier Mx_ , where x is a number. The individual files are: *.tdms contains columns of data (see below) listing each cell/event in row *para.ini contains parameters of the measurement, e.g. flow rate and channel size, pixel resolution ... *camera.ini contains settings to the camera, e.g. ROI and frame rate ... *.avi if available, collection of ROI images for every recorded event *contours.txt if available, collection of contour data (x and y position) for every recorded cell/object The following columns are found in *.tdms files: time frame number of a detected event (divide by frame rate to obtain time in s) x x position of the center of a detected object (in flow direction) in µm y y position of the center of a detected object (perpendicular to flow direction) in µm ax1 width of the detected object (in flow direction) in µm ax2 height of the detected object (perpendicular to flow direction)in µm circularity calculated from the convex hull of the object's contour by relating area within the convex hull A and perimeter p as circularity = 2*sqrt(pi*A)/p; deformation = 1 - circularity area area within the convex hull of the object's contour in px² (multiply by squared pixel resolution to obtain µm²) raw area area within the object's contour in px² (multiply by squared pixel resolution to obtain µm²)